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Achilles’ heel of pluripotent stem cells: genetic, genomic and epigenetic variations during prolonged culture
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“Genetic and epigenetic variants are known to arise in the derivation of a cell line and during cell culture expansion, notably in cancer cell lines and PSCs (Rebuzzini et al., 2016).”
From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Rebuzzini et al., 2016
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Microsatellite Instability in Colorectal Cancer
Boland, C. R., & Goel, A. (2010) · Gastroenterology · doi:10.1053/j.gastro.2009.12.064 · Cited by 2,456
The spindle-assembly checkpoint in space and time
Musacchio, A., & Salmon, E. D. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2163 · Cited by 2,227
Mechanisms of change in gene copy number
Hastings, P. J., Lupski, J. R., Rosenberg, S. M., & Ira, G. (2009) · Nature Reviews Genetics · doi:10.1038/nrg2593 · Cited by 1,316
Somatic coding mutations in human induced pluripotent stem cells
Gore, A., Li, Z., Fung, H.-L., Young, J. E., Agarwal, S., Antosiewicz-Bourget, J., Canto, I., Giorgetti, A., Israel, M. A., Kiskinis, E., et al. (2011) · Nature · doi:10.1038/nature09805 · Cited by 1,273
Laurent, L. C., Ulitsky, I., Slavin, I., Tran, H., Schork, A., Morey, R., Lynch, C., Harness, J. V., Lee, S., Barrero, M. J., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2010.12.003 · Cited by 890
The tumorigenicity of human embryonic and induced pluripotent stem cells
Ben-David, U., & Benvenisty, N. (2011) · Nature Reviews Cancer · doi:10.1038/nrc3034 · Cited by 889
Replication slippage involves DNA polymerase pausing and dissociation
Viguera, E., Canceill, D., & Ehrlich, S. D. (2001) · The EMBO Journal · doi:10.1093/emboj/20.10.2587 · Cited by 355
Karyotypic abnormalities in human induced pluripotent stem cells and embryonic stem cells
Taapken, S. M., Nisler, B. S., Newton, M. A., Sampsell-Barron, T. L., Leonhard, K. A., McIntire, E. M., & Montgomery, K. D. (2011) · Nature Biotechnology · doi:10.1038/nbt.1835 · Cited by 327
Garitaonandia, I., Amir, H., Boscolo, F. S., Wambua, G. K., Schultheisz, H. L., Sabatini, K., Morey, R., Waltz, S., Wang, Y.-C., Tran, H., et al. (2015) · PLOS ONE · doi:10.1371/journal.pone.0118307 · Cited by 150 · cited 1×
“Gain of entire chromosomes 12, 17, 20 or, to a lesser extent, 16 and X (particularly in female cell lines) frequently occurs in human ESCs (hESCs) [16–22], representing about 50 % of all aberrations observed [18].”
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Metadata
- Authors
- Rebuzzini, P., Zuccotti, M., Redi, C. A., & Garagna, S.
- Venue
- Cellular and Molecular Life Sciences
- Published
- 2016-03-09
- Volume
- 73
- Issue
- 13
- Pages
- 2453-2466
- ISSN
- 1420-682X
- 1420-9071
- Cited by
- 35
- References
- 9
- Language
- en
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- www.springer.com/tdm (tdm)
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