Examining the link between chromosomal instability and aneuploidy in human cells
Open accessAbstract
Solid tumors can be highly aneuploid and many display high rates of chromosome missegregation in a phenomenon called chromosomal instability (CIN). In principle, aneuploidy is the consequence of CIN, but the relationship between CIN and aneuploidy has not been clearly defined. In this study, we use live cell imaging and clonal cell analyses to evaluate the fidelity of chromosome segregation in chromosomally stable and unstable human cells. We show that improper microtubule–chromosome attachment (merotely) is a cause of chromosome missegregation in unstable cells and that increasing chromosome missegregation rates by elevating merotely during consecutive mitoses generates CIN in otherwise stable, near-diploid cells. However, chromosome missegregation compromises the proliferation of diploid cells, indicating that phenotypic changes that permit the propagation of nondiploid cells must combine with elevated chromosome missegregation rates to generate aneuploid cells with CIN.
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Chromosomally unstable cells exhibit evidence of chromosome missegregation during anaphase.
“Chromosomally unstable cells exhibit evidence of chromosome missegregation during anaphase3,4”
From Chromosomal instability drives metastasis through a cytosolic DNA response (2018) · cites this paper as [3]
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Self-citation
- Authors
- Thompson, S. L., & Compton, D. A.
- Affiliations
- Dartmouth College (US)
- Corresponding author
- Compton, Duane A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2008-02-18
- Volume
- 180
- Issue
- 4
- Pages
- 665-672
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2265570
- OpenAlex
- W2153304690
- Cited by
- 521
- References
- 1
- Citations by year
- 2026: 17 · 2025: 25 · 2024: 23 · 2023: 27 · 2022: 18 · 2021: 23 · 2020: 18 · 2019: 25 · 2018: 29 · 2017: 24 · 2016: 32 · 2015: 34 · 2014: 32 · 2013: 36 · 2012: 46
- FWCI
- 16.35
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM51542, R01GM051542, T32GM008704, R37GM051542)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomic variations and chromosomal abnormalities
- Keywords
- chromosomal instability, aneuploidy, chromosome missegregation, merotelic attachment, mitotic spindle, live-cell imaging, solid tumors, cell proliferation
- MeSH terms
- Aneuploidy, Cell Transformation, Neoplastic, Chromosomes, Clone Cells, Diploidy, Humans, Microtubules, Mitosis, Spindle Apparatus, Neoplasms, Gene Expression Regulation, Neoplastic, Microscopy, Video, Caco-2 Cells, Cell Lineage, Chromosome Segregation, Chromosomal Instability, Cell Line, Tumor
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