Microarray Profiling of Human Skeletal Muscle Reveals That Insulin Regulates ∼800 Genes during a Hyperinsulinemic Clamp
Open accessAbstract
Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. 478 were up-regulated and 284 down-regulated. Most of the genes with known function are novel targets of insulin. They are involved in the transcriptional and translational regulation (29%), intermediary and energy metabolisms (14%), intracellular signaling (12%), and cytoskeleton and vesicle traffic (9%). Other categories consisted of genes coding for receptors, carriers, and transporters (8%), components of the ubiquitin/proteasome pathways (7%) and elements of the immune response (5.5%). These results thus define a transcriptional signature of insulin action in human skeletal muscle. They will help to better define the mechanisms involved in the reduction of insulin effectiveness in pathologies such as type 2 diabetes mellitus, a disease characterized by defective regulation of gene expression in response to insulin.
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Recent microarray studies demonstrate an upregulation of OXPHOS genes after a short-term insulin infusion.
“Recent microarray studies demonstrate an upregulation of OXPHOS genes after a short-term insulin infusion (36).”
From A High-Fat Diet Coordinately Downregulates Genes Required for Mitochondrial Oxidative Phosphorylation in Skeletal Muscle (2005) · cites this paper as [36]
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- Authors
- Rome, S., Clément, K., Rabasa-Lhoret, R., Loizon, E., Poitou, C., Barsh, G. S., Riou, J.-P., Laville, M., & Vidal, H.
- Affiliations
- Inserm (FR)
- Corresponding author
- Rome, Sophie
- ORCID
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-05
- Volume
- 278
- Issue
- 20
- Pages
- 18063-18068
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2131470746
- Cited by
- 187
- References
- 48
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 8 · 2020: 2 · 2019: 8 · 2018: 4 · 2017: 6 · 2016: 5 · 2015: 6 · 2014: 7 · 2013: 7 · 2012: 5
- FWCI
- 7.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Keywords
- insulin action, skeletal muscle, hyperinsulinemic-euglycemic clamp, gene expression profiling, vastus lateralis, mRNA expression, type 2 diabetes, insulin resistance, transcriptional regulation, energy metabolism, ubiquitin-proteasome system, immune response
- MeSH terms
- Adult, Biological Transport, Cysteine Endopeptidases, Female, Gene Expression Regulation, Humans, Hyperinsulinism, Insulin, Male, Models, Biological, Multienzyme Complexes, Nucleic Acid Hybridization, RNA, RNA, Messenger, Transcription, Genetic, Protein Biosynthesis, Signal Transduction, Down-Regulation, Up-Regulation, DNA, Complementary, Muscle, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Ubiquitin, Proteasome Endopeptidase Complex
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