Cited by 57Adipose Tissue and MetabolismMetabolism, Diabetes, and CancerPeroxisome Proliferator-Activated Receptors

Amelioration of lipid-induced insulin resistance in rat skeletal muscle by overexpression of Pgc-1β involves reductions in long-chain acyl-CoA levels and oxidative stress

Open access
Wright, L. E., Brandon, A. E., Hoy, A. J., Forsberg, G.-B., Lelliott, C. J., Reznick, J., Löfgren, L., Oscarsson, J. +3 more·2011·Diabetologia

Abstract

Aims/hypothesis To determine if acute overexpression of peroxisome proliferator-activated receptor, gamma, coactivator 1 beta (Pgc-1β [also known as Ppargc1b]) in skeletal muscle improves insulin action in a rodent model of diet-induced insulin resistance.Methods Rats were fed either a low-fat or high-fat diet (HFD) for 4 weeks. In vivo electroporation was used to overexpress Pgc-1β in the tibialis cranialis (TC) and extensor digitorum longus (EDL) muscles. Downstream effects of Pgc-1β on markers of mitochondrial oxidative capacity, oxidative stress and muscle lipid levels were characterised. Insulin action was examined ex vivo using intact muscle strips and in vivo via a hyperinsulinaemic-euglycaemic clamp.Results Pgc-1β gene expression was increased >100% over basal levels. The levels of proteins involved in mitochondrial function, lipid metabolism and antioxidant defences, the activity of oxidative enzymes, and substrate oxidative capacity were all increased in muscles overexpressing Pgc-1β. In rats fed a HFD, increasing the levels of Pgc-1β partially ameliorated muscle insulin resistance, in association with decreased levels of long-chain acyl-CoAs (LCACoAs) and increased antioxidant defences.Conclusions Our data show that an increase in Pgc-1β expression in vivo activates a coordinated subset of genes that increase mitochondrial substrate oxidation, defend against oxidative stress and improve lipid-induced insulin resistance in skeletal muscle.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s00125-011-2068-x

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Upregulating PGC1β or activating heat shock protein 72 to increase mitochondrial function above the physiological adaptive response triggered by lipid oversupply mitigates lipid-induced insulin resistance.

    “In agreement with this, upregulating PGC 1β or activating heat shock protein 72 to increase mitochondrial function above the physiological adaptive response triggered by lipid oversupply mitigate lipid-induced insulin resistance (Wright et al., 2011; Henstridge et al., 2014).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

1 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wright, L. E., Brandon, A. E., Hoy, A. J., Forsberg, G.-B., Lelliott, C. J., Reznick, J., Löfgren, L., Oscarsson, J., Strömstedt, M., Cooney, G. J., & Turner, N.
Affiliations
  • Garvan Institute of Medical Research (AU)
  • AstraZeneca (Sweden) (SE)
Corresponding author
Turner, N.
Venue
Diabetologia
Published
2011-02-18
Volume
54
Issue
6
Pages
1417-1426
ISSN
  • 0012-186X (ISSN-L)
  • 1432-0428
OpenAlex
W2151974497
Cited by
57
References
50
Citations by year
2026: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 5 · 2016: 7 · 2015: 5 · 2014: 5 · 2013: 8 · 2012: 6
FWCI
4.80
Type
journal article
Language
en
Open access
Locations
  • Diabetologia (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of New South Wales
  • Medical Research Council
  • National Health and Medical Research Council
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
PGC-1β, PPARGC1B, insulin resistance, skeletal muscle, high-fat diet, long-chain acyl-CoA, oxidative stress, mitochondrial function, antioxidant defense, in vivo electroporation, hyperinsulinemic-euglycemic clamp, lipid metabolism
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Acyl Coenzyme A, Animals, Dietary Fats, Insulin Resistance, Male, Mitochondria, Muscle, Time Factors, Transcription Factors, RNA-Binding Proteins, Rats, Wistar, Reactive Oxygen Species, Oxidative Stress, Muscle, Skeletal, Models, Animal, Lipid Metabolism, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review