The Specific Force of Single Intact Extensor Digitorum Longus and Soleus Mouse Muscle Fibers Declines with Aging
Abstract
In the present study we measured, for the first time, the isometric specific force (SF, force normalized to cross sectional area) generated by single intact fibers from fast- (extensor digitorum longus, EDL) and slow-twitch (soleus) muscles from young adult (2-6), middle-aged (12-14) and old (20-24 month-old) mice. SF has also been measured in single intact flexor digitorum brevis fibers from young mice. Muscle fibers have been classified into fast- or slow-twitch based on the contraction kinetics. Maximum SF recorded in EDL and soleus fibers from young and middle-aged mice did not differ significantly. A significant age-dependent decline in maximum SF was recorded in EDL and soleus fibers from young or middle-aged to old mice. The SF was 377 +/- 18, 417 +/- 20 and 279 +/- 18 kPa for EDL fibers from young, middle-aged and old mice, respectively and 397 +/- 17, 405 +/- 24 and 320 +/- 33 kPa for soleus fibers from age-matched mice, respectively. The frequency needed to elicit maximum force in EDL and soleus fibers from middle-aged to old mice did not differ significantly. In conclusion, the specific force developed by both fast and slow-twitch single intact muscle fibers declines with aging and more significantly in the former.
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Loss of muscle performance has a greater negative impact on older adults than loss of muscle mass.
“the preponderance of evidence from years indicates that loss of muscle performance has a greater negative impact on older adults than loss of mass [18, 20–25, 37–39].”
From Targeting Intramyocellular Lipids to Improve Aging Muscle Function (2025) · cites this paper as [21]
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Age-dependent IGF-1 regulation of gene transcription of Ca2+ channels in skeletal muscle
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Self-citationIncreased CaVβ1a expression with aging contributes to skeletal muscle weakness
Taylor, J. R., Zheng, Z., Wang, Z., Payne, A. M., Messi, M. L., & Delbono, O. (2009) · Aging Cell · doi:10.1111/j.1474-9726.2009.00507.x · Cited by 25 · see citing works
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Self-citationAge-related changes in muscles and joints
Ahmed, M. S., Matsumura, B. A., & Cristian, A. (2005) · Physical Medicine and Rehabilitation Clinics of North America · doi:10.1016/j.pmr.2004.06.017 · Cited by 25 · see citing works
Increased desmin expression in hindlimb muscles of aging rats
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Measurement of Skeletal Muscle Fiber Contractility with High-Speed Traction Microscopy
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Effects of alcohol on skeletal muscle contractile performance in male and female mice
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Soft Biological Actuators for Meter-Scale Homeostatic Biohybrid Robots
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Recovery from fatigue in fast and slow single intact skeletal muscle fibers from aging mouse
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Targeting Intramyocellular Lipids to Improve Aging Muscle Function
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Troponin T3 regulates nuclear localization of the calcium channel Cavβ1a subunit in skeletal muscle
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- Authors
- González, E., Messi, M. L., & Delbono, O.
- Affiliations
- Wake Forest University Health Sciences (US)
- ORCID
- Delbono, O.
- Venue
- Journal of Membrane Biology
- Published
- 2000-12-01
- Volume
- 178
- Issue
- 3
- Pages
- 175-183
- ISSN
- 0022-2631 (ISSN-L)
- 1432-1424
- OpenAlex
- W1965850822
- Cited by
- 115
- References
- 40
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 7 · 2013: 4 · 2012: 9
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- The Journal of Membrane Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG 15820, AG-10484, P60AG010484, AG00692, AG-13934, R01AG015820)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- muscle aging, extensor digitorum longus, soleus muscle, flexor digitorum brevis, fast-twitch muscle, slow-twitch muscle, isometric force, contraction kinetics
- MeSH terms
- Aging, Animals, Female, Isometric Contraction, Kinetics, Male, Mice, Inbred DBA, Muscle Contraction, Specific Pathogen-Free Organisms, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Muscle Fibers, Slow-Twitch, Myosin Heavy Chains, Protein Isoforms, Muscle Development, Mice
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