Cited by 293Microplastics and Plastic PollutionEnvironmental Toxicology and EcotoxicologyPharmaceutical and Antibiotic Environmental Impacts

Detection of Anthropogenic Particles in Fish Stomachs: An Isolation Method Adapted to Identification by Raman Spectroscopy

Collard, France, Gilbert, Bernard, Eppe, Gauthier, Parmentier, Eric, Das, Krishna·2015·Archives of Environmental Contamination and Toxicology

Abstract

Microplastic particles (MP) contaminate oceans and affect marine organisms in several ways. Ingestion combined with food intake is generally reported. However, data interpretation often is circumvented by the difficulty to separate MP from bulk samples. Visual examination often is used as one or the only step to sort these particles. However, color, size, and shape are insufficient and often unreliable criteria. We present an extraction method based on hypochlorite digestion and isolation of MP from the membrane by sonication. The protocol is especially well adapted to a subsequent analysis by Raman spectroscopy. The method avoids fluorescence problems, allowing better identification of anthropogenic particles (AP) from stomach contents of fish by Raman spectroscopy. It was developed with commercial samples of microplastics and cotton along with stomach contents from three different Clupeiformes fishes: Clupea harengus, Sardina pilchardus, and Engraulis encrasicolus. The optimized digestion and isolation protocol showed no visible impact on microplastics and cotton particles while the Raman spectroscopic spectrum allowed the precise identification of microplastics and textile fibers. Thirty-five particles were isolated from nine fish stomach contents. Raman analysis has confirmed 11 microplastics and 13 fibers mainly made of cellulose or lignin. Some particles were not completely identified but contained artificial dyes. The novel approach developed in this manuscript should help to assess the presence, quantity, and composition of AP in planktivorous fish stomachs.

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Crossref · 10.1007/s00244-015-0221-0

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47 of 47 references verified0 citation errors · 0 not checkable

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  • Natural and semisynthetic microfibers have been found in wild marine organisms.

    “Recently, some studies underlined the presence of natural and semisynthetic microfibers in wild marine organisms [86,87,88,89].”

    From Is Wild Marine Biota Affected by Microplastics? (2022) · cites this paper as [88]

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Authors
Collard, F., Gilbert, B., Eppe, G., Parmentier, E., & Das, K.
Affiliations
University of Liège (BE)
Corresponding author
  • Collard, France
  • Das, Krishna
Venue
Archives of Environmental Contamination and Toxicology
Published
2015-08-20
Volume
69
Issue
3
Pages
331-339
ISSN
  • 0090-4341 (ISSN-L)
  • 1432-0703
OpenAlex
W2151512058
Cited by
293
References
47
Citations by year
2026: 10 · 2025: 17 · 2024: 30 · 2023: 22 · 2022: 42 · 2021: 33 · 2020: 37 · 2019: 28 · 2018: 28 · 2017: 28 · 2016: 15 · 2015: 3
FWCI
15.36
Type
journal article
Language
en
Open access
closed
Locations
  • Archives of Environmental Contamination and Toxicology (publishedVersion)
  • PubMed (publishedVersion)
  • Flanders Marine Institute (Flanders Marine Institute) (submittedVersion)
  • ORBi (University of Liège) (submittedVersion)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
microplastics, anthropogenic particles, fish stomach contents, particle isolation, Raman spectroscopy, textile fibers, herring, Sardina pilchardus, Engraulis encrasicolus, planktivorous fish, marine plastic pollution, synthetic dyes
MeSH terms
Animals, Environmental Monitoring, Fishes, Gastrointestinal Contents, Plastics, Spectrum Analysis, Raman, Water Pollutants, Chemical

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