Cited by 56Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyAnaerobic Digestion and Biogas Production

Moringa oleifera oil modulates rumen microflora to mediate in vitro fermentation kinetics and methanogenesis in total mix rations

Ebeid, Hossam M., Mengwei, Li, Kholif, Ahmed E., Hassan, Faiz-ul, Lijuan, Peng, Xin, Liang, Chengjian, Yang·2020·Current Microbiology

Abstract

This study was conducted to evaluate potential of Moringa oleifera seed oil (MOSO) to modulate rumen microflora to mitigate methane (CH 4) production in different total mixed rations (TMRs). Three TMRs with different roughage (R) to concentrate (C) ratio were used as substrates (R70:C30, R50:C50 and R30:C70) for in vitro fermentation study using batch culture technique. Results revealed that supplementation of MOSO in different rations with variable roughage to concentrates ratio altered (P < 0.05) CH 4 production and fermentation parameters. M. oleifera seed oil at 3 and 4% increased (P < 0.01) microbial protein (MCP) and propionate concentration for all rations but decreased acetate concentration in R70:C30 TMR. Two levels of MOSO (3 and 4%) quadratically increased (P = 0.001) MCP and decreased acetate in R30:C70 while all levels increased propionate. For R30:C70 and R50:C50 rations, MOSO linearly and quadratically decreased (P < 0.001) protozoa and methanogen counts; however, protozoa, methanogens and bacteria were significantly increased in R70:C30 ration without any change in fungal counts. Supplementation of higher level of MOSO (4%) in high roughage ration (R70:C30) showed negative effects on diversity (Shannon index) and evenness of bacterial species as compared to control and lower oil level. Moreover, it also decreased Firmicutes to Bacteroidetes ratio in high roughage rations more obviously at lower levels. Moringa oil also stimulated Prevotella in both high and low roughages diets that indicates its potential to mediate rumen acidosis. Conclusively, MOSO enhanced fermentation kinetics and decreased CH 4 production through effective modulation of rumen microbiome.

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Crossref · 10.1007/s00284-020-01935-2

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Authors
Ebeid, H. M., Mengwei, L., Kholif, A. E., Hassan, F.-U., Lijuan, P., Xin, L., & Chengjian, Y.
Affiliations
  • Guangxi Buffalo Research Institute (CN)
  • National Research Centre (EG)
Corresponding author
Chengjian, Yang
Venue
Current Microbiology
Published
2020-03-04
Volume
77
Issue
7
Pages
1271-1282
ISSN
  • 0343-8651 (ISSN-L)
  • 1432-0991
OpenAlex
W3010200042
Cited by
56
References
45
Citations by year
2026: 4 · 2025: 4 · 2024: 13 · 2023: 8 · 2022: 11 · 2021: 7 · 2020: 9
FWCI
10.94
Type
journal article
Language
en
Open access
closed
Locations
  • Current Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Key Research and Development Program of China (2016YFD0500507, 2018YFD0501600)
  • National Natural Science Foundation of China (31560649)
  • Natural Science Foundation of Guangxi Province (2018GXNSFAA281162)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords
Moringa oleifera seed oil, rumen microbiota, methane mitigation, in vitro rumen fermentation, total mixed ration, roughage to concentrate ratio, microbial protein, volatile fatty acids, propionate, acetate, methanogenic archaea, rumen protozoa, rumen bacteria, rumen acidosis, Prevotella, Firmicutes/Bacteroidetes ratio, rumen fermentation kinetics
MeSH terms
Gastrointestinal Microbiome, Animals, Bacteria, Buffaloes, Fermentation, Kinetics, Methane, Models, Biological, Rumen, Moringa oleifera

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