Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats
Abstract
It is well known that exhaustive exercise increases serum and skeletal muscle IL-6 concentrations. However, the effect of exhaustive exercise on the concentrations of other cytokines in the muscle and in the adipose tissue is controversial. The purpose of this study was to evaluate the effect of exhaustive exercise on mRNA and protein expression of IL-10, TNF-alpha and IL-6 in different types of skeletal muscle (EDL, soleus) and in two different depots of white adipose tissue (mesenteric-MEAT and retroperitoneal-RPAT). Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6) and 6 (E6 group, n = 6) hours after the exhaustion protocol, which consisted of running on a treadmill (approximately 70% VO(2max) for 50 min and then subsequently at an elevated rate that increased at 1 m/min every minute, until exhaustion). The control group (C group, n = 6) was not subjected to exercise. Cytokine protein expression increased in EDL, soleus, MEAT and RPAT from all exercised groups, as detected by ELISA. EDL IL-10 and TNF-alpha expression was higher than that of the soleus. The IL-10/TNF-alpha ratio was increased in the skeletal muscle, especially in EDL, but it was found to be decreased in the adipose tissue. These results show that exhaustive exercise presents a different effect depending on the tissue which is analysed: in the muscle, it induces an anti-inflammatory effect, especially in type 2 fibres, while the pro-inflammatory effect prevails in adipose tissue, possibly contributing to increased lipolysis to provide energy for the exercising muscle.
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Regular physical exercise is directly related to reduced systemic inflammation.
“(5) reduced systemic inflammation (Lira et al., 2009; Rosa Neto et al., 2009; Batista et al., 2010)”
From Physical Exercise-Induced Myokines and Muscle-Adipose Tissue Crosstalk: A Review of Current Knowledge and the Implications for Health and Metabolic Diseases (2018) · cites this paper as Rosa Neto et al., 2009
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A INGESTÃO DIETÉTICA DA POLPA DE AÇAÍ (Euterpe oleracea) COMO SUPLEMENTO ALIMENTAR PRÉ-TREINO
Ricciard, É. D. S. P., Rodrigues, A. L. D. S., de Moura, R. S., Souza, M. L. D. S., Júnior, S. L. P., da Silva, C. F., de Abreu, L. C., Barros, J. D. S. V., & Silva, R. P. M. (2024) · Stricto Sensu Editora eBooks · doi:10.35170/ss.ed.9786580261321.01
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- Authors
- Rosa Neto, J. C., Lira, F. S., Oyama, L. M., Zanchi, N. E., Yamashita, A. S., Batista, M. L., Oller do Nascimento, C. M., & Seelaender, M.
- Affiliations
- Universidade de São Paulo (BR)
- Universidade Federal de São Paulo (BR)
- Venue
- European Journal of Applied Physiology
- Published
- 2009-05-08
- Volume
- 106
- Issue
- 5
- Pages
- 697-704
- ISSN
- 0020-9376 (ISSN-L)
- 1439-6319
- 1439-6327
- OpenAlex
- W2112842295
- Cited by
- 112
- References
- 53
- Citations by year
- 2025: 5 · 2024: 13 · 2023: 4 · 2022: 4 · 2021: 6 · 2020: 5 · 2019: 2 · 2018: 4 · 2017: 9 · 2016: 6 · 2015: 9 · 2014: 6 · 2013: 10 · 2012: 7
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
- UNIFESP Institutional Repository (Universidade Federal de São Paulo) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- exhaustive exercise, inflammation, skeletal muscle, adipose tissue, IL-10, TNF-α, interleukin-6, cytokine expression, soleus muscle, EDL muscle, mesenteric adipose tissue, retroperitoneal adipose tissue, lipolysis, treadmill running
- MeSH terms
- Adipose Tissue, Animals, Physical Exertion, Inflammation, Lipolysis, Male, Physical Conditioning, Animal, Tumor Necrosis Factor-alpha, Interleukin-6, Interleukin-10, Rats, Wistar, Muscle, Skeletal, Rats
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