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Cited by 13Muscle metabolism and nutritionMuscle Physiology and DisordersCardiovascular and exercise physiology

Effects of immobilization and aerobic training on proteins related to intramuscular substrate storage and metabolism in young and older men

Vigelsø, Andreas, Gram, Martin, Wiuff, Caroline, Hansen, Christina Neigaard, Prats, Clara, Dela, Flemming, Helge, Jørn Wulff·2016·European Journal of Applied Physiology
DOI 10.1007/s00421-015-3302-x
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Abstract

Purpose Aging and inactivity lead to skeletal muscle metabolic inflexibility, but the underlying molecular mechanisms are not entirely elucidated. Therefore, we investigated how muscle lipid and glycogen stores and major regulatory proteins were affected by short-term immobilization followed by aerobic training in young and older men.Methods 17 young (23 ± 1 years, 24 ± 1 kg m(-2), and 20 ± 2% body fat) and 15 older men (68 ± 1 years; 27 ± 1 kg m(-2), and 29 ± 2% body fat) underwent 2 weeks' one leg immobilization followed by 6 weeks' cycle training. Biopsies were obtained from m. vastus lateralis just before immobilization (at inclusion), after immobilization, and the after 6 weeks' training. The biopsies were analyzed for muscle substrates; muscle perilipin protein (PLIN), glycogen synthase (GS), synaptosomal-associated protein of 23 kDa (SNAP23) protein content, and muscle 3-hydroxyacyl-CoA dehydrogenase (HAD) activity Results The older men had higher intramuscular triglyceride (IMTG) (73 %) and Glycogen (16%) levels compared to the young men, and IMTG tended to increase with immobilization. PLIN2 and 3 protein content increased with immobilization in the older men only. The young men had higher GS (74%) protein compared to the older men. Immobilization decreased and training restored HAD activity, GS and SNAP23 protein content in young and older men.Conclusion Evidence of age-related metabolic inflexibility is presented, seen as body fat and IMTG accumulation. The question arises as to whether IMTG accumulation in the older men is caused by or leading to the increase in PLIN2 and 3 protein content. Training decreased body fat and IMTG levels in both young and older men; hence, training should be prioritized to reduce the detrimental effect of aging on metabolism.

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  • Aging and disuse, even in the absence of insulin resistance, are associated with increased intramyocellular lipids.

    “However, aging and disuse, even in the absence of insulin resistance are associated with increased IMCLs [57, 60–63].”

    From Targeting Intramyocellular Lipids to Improve Aging Muscle Function (2025) · cites this paper as [60]

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Authors
Vigelsø, A., Gram, M., Wiuff, C., Hansen, C. N., Prats, C., Dela, F., & Helge, J. W.
Affiliations
University of Copenhagen (DK)
Corresponding author
Vigelsø, Andreas
ORCID
Venue
European Journal of Applied Physiology
Publisher
Springer Science and Business Media LLC
Published
2015-12-01
Volume
116
Issue
3
Pages
481-494
ISSN
  • 0020-9376 (ISSN-L)
  • 1439-6319
  • 1439-6327
DOI
10.1007/s00421-015-3302-x
OpenAlex
W2182225887
Cited by
13
References
81
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2021: 2 · 2018: 1 · 2017: 5 · 2016: 1
FWCI
1.24
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • European Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
immobilization, aerobic exercise, aging, intramuscular triglycerides, glycogen, perilipin, glycogen synthase, SNAP23, 3-hydroxyacyl-CoA dehydrogenase, vastus lateralis, fat mass, older men
MeSH terms
Perilipin-1, Aged, Carrier Proteins, Glycogen, Glycogen Synthase, Humans, Immobilization, Male, Muscle Proteins, Phosphoproteins, Exercise, Muscle, Skeletal, Lipid Metabolism, Qb-SNARE Proteins, Qc-SNARE Proteins, Young Adult, 3-Hydroxyacyl-CoA Dehydrogenase

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