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Cited by 52Muscle Physiology and DisordersMuscle metabolism and nutritionNutrition and Health in Aging

Short-term muscle disuse atrophy is not associated with increased intramuscular lipid deposition or a decline in the maximal activity of key mitochondrial enzymes in young and older males

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Wall, Benjamin T., Dirks, Marlou L., Snijders, Tim, Stephens, Francis B., Senden, Joan M.G., Verscheijden, Marie-Louise, van Loon, Luc J.C.·2015·Experimental Gerontology
DOI 10.1016/j.exger.2014.11.019
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Abstract

Aging is generally accompanied by a progressive loss of skeletal muscle mass and impairments in metabolic function. Even a few days of muscle disuse (such as that occurring during injury or illness) leads to considerable loss of muscle mass and strength. It has been speculated that short, successive periods of muscle disuse throughout the lifespan may be largely responsible for the age-related loss of muscle mass. However, it remains unknown whether such short periods of disuse also induce impairments in metabolic function within skeletal muscle. Here, we investigated the effects of a five day period of muscle disuse on intramyocellular triacylglycerol (IMTG) content, muscle oxidative capacity, and the expression of key genes that regulate oxidative metabolism in healthy young and elderly men. Muscle biopsies were collected from healthy, young (n=12; 23±1y) and elderly (n=12; 70±1y) men prior to and immediately after a five day period of one-legged knee immobilization by way of a full leg cast. At baseline, elderly men had a greater IMTG content when compared with the young (56.2±5.1 and 34.8±7.3μmol·g(-1), respectively; P<0.05) with no changes in either group following immobilization (53.4±5.0 and 35.7±5.0μmol·g(-1), respectively; P>0.05). In line, five days of disuse did not lower citrate synthase, β-HAD or cytochrome C oxidase activity in skeletal muscle tissue. Pyruvate dehydrogenase activity increased following immobilization in the older subjects only, from 0.39±0.06 to 0.55 0.05μmol·g(-1)·min(-1) (71±33%; P<0.01). The skeletal muscle mRNA expression of PGC1α and citrate synthase both declined following immobilization in both the young and elderly subjects. We conclude that five days of muscle disuse does not increase intramuscular lipid deposition or reduce the maximal activity of key mitochondrial enzymes within the skeletal muscle of young or older men.

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Authors
Wall, B. T., Dirks, M. L., Snijders, T., Stephens, F. B., Senden, J. M. G., Verscheijden, M.-L., & van Loon, L. J. C.
Affiliations
  • Maastricht University Medical Centre (NL)
  • University of Nottingham (GB)
ORCID
Venue
Experimental Gerontology
Publisher
Elsevier BV
Published
2015-01
Volume
61
Pages
76-83
ISSN
0531-5565 (ISSN-L)
DOI
10.1016/j.exger.2014.11.019
OpenAlex
W2050296036
Cited by
52
References
73
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 2 · 2022: 6 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 6
FWCI
2.19
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Gerontology (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion)
  • Open Research Exeter (University of Exeter) (acceptedVersion)
  • Research Bank (Australian Catholic University) (submittedVersion)
  • Figshare (submittedVersion)
  • Socio-Environmental Systems Modeling (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
Funders
Medical Research Council (MR/K00414X/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords
muscle disuse, skeletal muscle atrophy, mitochondrial enzymes, citrate synthase, pyruvate dehydrogenase, cytochrome c oxidase, beta-hydroxyacyl-CoA dehydrogenase, PGC-1α, oxidative metabolism, knee immobilization, aging, sarcopenia, mitochondrial biogenesis
MeSH terms
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Adult, Aged, Aging, Body Composition, Citrate (si)-Synthase, Humans, Insulin Resistance, Male, Mitochondria, Muscle, Muscular Atrophy, Pyruvate Dehydrogenase Complex, RNA, Messenger, Transcription Factors, Triglycerides, Muscle, Skeletal, Lipid Metabolism, Muscle Strength

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