Cited by 45Transgenic Plants and ApplicationsPlant tissue culture and regenerationPlant Molecular Biology Research

Creation of ecdysone receptor chimeras in plants for controlled regulation of gene expression

Martinez, A., Sparks, C., Drayton, P., Thompson, J., Greenland, A., Jepson, I.·1999·Molecular and General Genetics MGG

Abstract

Transformation with a chimeric receptor containing the glucocorticoid transactivation and DNA-binding domains fused to an ecdysteroid receptor ligand-binding domain permits ecdysone agonist-inducible gene expression in monocotyledonous plant cells. The inducible system is based on the specific activation of a chimeric receptor containing the ligand-binding domain of the Heliothis virescens ecdysteroid receptor and the inducer RH5992 (a 20-hydroxyecdysone agonist). RH5992 is an non-steroidal agrochemical with a high specificity for lepidopteran ecdysone receptors. Addition of RH5992 to transformed cells results in high levels of inducible expression in a ligand-specific manner, particularly when the effector receptor is coupled to the strong transactivator VP16. A chimeric construct containing the Drosophila ecdysone ligand-binding domain failed to activate reporter gene activity with RH5992, while activation was observed in the presence of muristeroneA. The system described provides the basis for an inducible gene expression system that is compatible with agricultural use.

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Crossref · 10.1007/s004380050999

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31 of 31 references verified0 citation errors · 0 not checkable

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Authors
Martinez, A., Sparks, C., Drayton, P., Thompson, J., Greenland, A., & Jepson, I.
Affiliations
AstraZeneca (United Kingdom) (GB)
Venue
Molecular and General Genetics MGG
Published
1999-04
Volume
261
Issue
3
Pages
546-552
ISSN
  • 0026-8925 (ISSN-L)
  • 1432-1874
OpenAlex
W2024305397
Cited by
45
References
31
Citations by year
2020: 1 · 2017: 1 · 2015: 1 · 2012: 1
FWCI
3.25
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and General Genetics MGG (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
ecdysone receptor, chimeric receptors, inducible gene expression, ligand-binding domain, DNA-binding domain, Heliothis virescens, VP16, transgenic plants, plant transformation
MeSH terms
Chimera, Zea mays, Drosophila, Ecdysone, Ecdysterone, Lepidoptera, Molecular Sequence Data, Receptors, Glucocorticoid, Receptors, Steroid, Transformation, Genetic, Transcriptional Activation, Genes, Plant, Genes, Insect, Herpes Simplex Virus Protein Vmw65, Genes, Reporter, Simplexvirus, Gene Expression Regulation, Plant

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