Creation of ecdysone receptor chimeras in plants for controlled regulation of gene expression
Abstract
Transformation with a chimeric receptor containing the glucocorticoid transactivation and DNA-binding domains fused to an ecdysteroid receptor ligand-binding domain permits ecdysone agonist-inducible gene expression in monocotyledonous plant cells. The inducible system is based on the specific activation of a chimeric receptor containing the ligand-binding domain of the Heliothis virescens ecdysteroid receptor and the inducer RH5992 (a 20-hydroxyecdysone agonist). RH5992 is an non-steroidal agrochemical with a high specificity for lepidopteran ecdysone receptors. Addition of RH5992 to transformed cells results in high levels of inducible expression in a ligand-specific manner, particularly when the effector receptor is coupled to the strong transactivator VP16. A chimeric construct containing the Drosophila ecdysone ligand-binding domain failed to activate reporter gene activity with RH5992, while activation was observed in the presence of muristeroneA. The system described provides the basis for an inducible gene expression system that is compatible with agricultural use.
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Phytoecdysteroids can alter and switch genes in plants through DNA binding.
“These chemicals are also able to alter and switch genes around in plants through DNA binding10,11.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [11]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R., Kavanagh, T. A., & Bevan, M. W. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02730.x · Cited by 9,854 · see citing works
The scanning model for translation: an update.
Kozak, M. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.2.229 · Cited by 3,485 · see citing works
Primary structure and expression of a functional human glucocorticoid receptor cDNA
Hollenberg, S. M., Weinberger, C., Ong, E. S., Cerelli, G., Oro, A. E., Lebo, R. V., Thompson, E. B., Rosenfeld, M. G., & Evans, R. M. (1985) · Nature · doi:10.1038/318635a0 · Cited by 1,871 · see citing works
Koelle, M. R., Talbot, W. S., Segraves, W. A., Bender, M. T., Cherbas, P. T., & Hogness, D. S. (1991) · Cell · doi:10.1016/0092-8674(91)90572-g · Cited by 978 · see citing works
Functional ecdysone receptor is the product of EcR and Ultraspiracle genes
Yao, T., FORMAN, B. M., Jiang, Z., Cherbas, L., Chen, J.-D., McKeown, M., Cherbas, P. T., & Evans, R. M. (1993) · Nature · doi:10.1038/366476a0 · Cited by 947 · see citing works
A glucocorticoid‐mediated transcriptional induction system in transgenic plants
Aoyama, T., & Chua, N. (1997) · The Plant Journal · doi:10.1046/j.1365-313x.1997.11030605.x · Cited by 940 · see citing works
Ecdysone-inducible gene expression in mammalian cells and transgenic mice.
No, D. B., Yao, T. P., & Evans, R. M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3346 · Cited by 852 · see citing works
Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.
Furth, P. A., St‐Onge, L., Boeger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H., & Hennighausen, L. G. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.20.9302 · Cited by 768 · see citing works
Drosophila ultraspiracle modulates ecdysone receptor function via heterodimer formation
Yao, T., Segraves, W. A., Oro, A. E., McKeown, M., & Evans, R. M. (1992) · Cell · doi:10.1016/0092-8674(92)90266-f · Cited by 716 · see citing works
Hybrid genes in the analysis of transformation conditions
Negrutiu, I., Shillito, R. D., Potrykus, I., Biasini, M. G., & Florin, S. A. L. A. (1987) · Plant Molecular Biology · doi:10.1007/bf00015814 · Cited by 524 · see citing works
Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle
Thomas, H. E., Stunnenberg, H. G., & Stewart, A. F. (1993) · Nature · doi:10.1038/362471a0 · Cited by 520 · see citing works
Chimaeras of Myc oncoprotein and steroid receptors cause hormone-dependent transformation of cells
Eilers, M., Picard, D., Yamamoto, K. R., & Bishop, J. M. (1989) · Nature · doi:10.1038/340066a0 · Cited by 458 · see citing works
Activation of floral meristem identity genes in Arabidopsis
Simon, R. G., Igeño, M. I., & Coupland, G. (1996) · Nature · doi:10.1038/384059a0 · Cited by 378 · see citing works
Goff, S. A., Klein, T. M., Roth, B. A., et al. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb07431.x · Cited by 256 · see citing works
Epidermal Cell Fate Determination in Arabidopsis : Patterns Defined by a Steroid-Inducible Regulator
Lloyd, A., Schena, M., Walbot, V., et al. (1994) · Science · doi:10.1126/science.7939683 · Cited by 249 · see citing works
Gatz, C., Frohberg, C., & Wendenburg, R. (1992) · The Plant Journal · doi:10.1111/j.1365-313x.1992.00397.x · Cited by 236 · see citing works
Control of gene activity in higher eukaryotic cells by prokaryotic regulatory elements
Gossen, M., Bonin, A. L., & Bujard, H. (1993) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(93)90009-c · Cited by 229 · see citing works
Aoyama, T., Dong, C. H., Wu, Y., et al. (1995) · The Plant Cell · doi:10.1105/tpc.7.11.1773 · Cited by 220 · see citing works
A chimeric transactivator allows tetracycline-responsive gene expression in whole plants
Weinmann, P., Gossen, M., Hillen, W., Bujard, H., & Gatz, C. (1994) · The Plant Journal · doi:10.1046/j.1365-313x.1994.5040559.x · Cited by 196 · see citing works
A steroid-inducible gene expression system for plant cells.
Schena, M., Lloyd, A. M., & Davis, R. W. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10421 · Cited by 171 · see citing works
Production of fertile transgenic maize plants by silicon carbide whisker‐mediated transformation
Frame, B. R., Drayton, P. R., Bagnall, S. V., Lewnau, C. J., Bullock, W. P., Wilson, H. M., Dunwell, J. M., Thompson, J. A., & Wang, K. (1994) · The Plant Journal · doi:10.1046/j.1365-313x.1994.6060941.x · Cited by 161 · see citing works
Self-citationChristopherson, K. S., Mark, M. R., Bajaj, V., & Godowski, P. J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.14.6314 · Cited by 160 · see citing works
Yeast activators stimulate plant gene expression
Ma, J., Przibilla, E., Hu, J., Bogorad, L., & Ptashne, M. (1988) · Nature · doi:10.1038/334631a0 · Cited by 160 · see citing works
Copper-controllable gene expression system for whole plants.
Mett, V. L., Lochhead, L. P., & Reynolds, P. H. S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.10.4567 · Cited by 148 · see citing works
Chemical Regulation of Bacillus Thuringiensis ∂-Endotoxin Expression in Transgenic Plants
Williams, S. C., Friedrich, L., Dincher, S. S., et al. (1992) · Nature Biotechnology · doi:10.1038/nbt0592-540 · Cited by 98 · see citing works
Rieping, M., Fritz, M., Prat, S., et al. (1994) · The Plant Cell · doi:10.1105/tpc.6.8.1087 · Cited by 56 · see citing works
Control of gene expression in tobacco cells using a bacterial operator‐repressor system.
Wilde, R., Shufflebottom, D., Cooke, S. E., et al. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05169.x · Cited by 50 · see citing works
Blackford, M., & Dinan, L. N. (1997) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(96)00080-x · Cited by 36 · see citing works
An auto-inducible vector conferring high glucocorticoid inducibility upon stable transformant cells
Ko, M. S. H., Naomi, T., Norifumi, S., et al. (1989) · Gene · doi:10.1016/0378-1119(89)90512-x · Cited by 33 · see citing works
Control of gene expression in plant cells using a 434:VP16 chimeric protein
Wilde, R., Cooke, S. E., Brammar, W. J., et al. (1994) · Plant Molecular Biology · doi:10.1007/bf00020175 · Cited by 22 · see citing works
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Riddiford, L. M., Cherbas, P., & Truman, J. W. (2000) · Vitamins & Hormones · doi:10.1016/s0083-6729(00)60016-x · Cited by 605 · see citing works
Phytoecdysteroids: Biological aspects
Dinan, L. (2001) · Phytochemistry · doi:10.1016/s0031-9422(01)00078-4 · Cited by 392 · see citing works
Practical uses for ecdysteroids in mammals including humans: and update
Lafont, R., & Dinan, L. (2003) · Journal of Insect Science · doi:10.1093/jis/3.1.7 · Cited by 196 · see citing works
Practical uses for ecdysteroids in mammals including humans: And update
Lafont, R., & Dinan, L. (2003) · Journal of Insect Science · doi:10.1673/031.003.0701 · Cited by 196 · see citing works
The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review
Das, N., Mishra, S. K., Bishayee, A., Ali, E. S., & Bishayee, A. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2020.10.012 · Cited by 115 · see citing works
Ecdysteroid Structure-Activity Relationships
(2003) · Studies in natural products chemistry · doi:10.1016/s1572-5995(03)80004-2 · Cited by 35 · see citing works
- Authors
- Martinez, A., Sparks, C., Drayton, P., Thompson, J., Greenland, A., & Jepson, I.
- Affiliations
- AstraZeneca (United Kingdom) (GB)
- ORCID
- Sparks, C.
- Venue
- Molecular and General Genetics MGG
- Published
- 1999-04
- Volume
- 261
- Issue
- 3
- Pages
- 546-552
- ISSN
- 0026-8925 (ISSN-L)
- 1432-1874
- OpenAlex
- W2024305397
- Cited by
- 45
- References
- 31
- Citations by year
- 2020: 1 · 2017: 1 · 2015: 1 · 2012: 1
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Molecular and General Genetics MGG (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- ecdysone receptor, chimeric receptors, inducible gene expression, ligand-binding domain, DNA-binding domain, Heliothis virescens, VP16, transgenic plants, plant transformation
- MeSH terms
- Chimera, Zea mays, Drosophila, Ecdysone, Ecdysterone, Lepidoptera, Molecular Sequence Data, Receptors, Glucocorticoid, Receptors, Steroid, Transformation, Genetic, Transcriptional Activation, Genes, Plant, Genes, Insect, Herpes Simplex Virus Protein Vmw65, Genes, Reporter, Simplexvirus, Gene Expression Regulation, Plant
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