Cited by 22Osteoarthritis Treatment and MechanismsBone Metabolism and DiseasesProteoglycans and glycosaminoglycans research

Differential regulation of cellular maturation in chondrocytes and osteoblasts by glycine

Takahata, Yoshifumi, Takarada, Takeshi, Osawa, Masato, Hinoi, Eiichi, Nakamura, Yukari, Yoneda, Yukio·2008·Cell and Tissue Research

Abstract

Previous studies have demonstrated the functional expression, by osteoblasts, of N-methyl-D-aspartate (NMDA) receptors responsible for the promotion of cellular differentiation in bone. We have now evaluated the possible role of the endogenous co-agonist of NMDA receptors, glycine (Gly), in chondrogenesis. In ex vivo organotypic cultures of fetal mouse tibias, proximal and distal cartilaginous primordia were significantly increased in the presence of Gly, with the osteogenic center being unchanged. Exposure to Gly drastically increased mRNA expression of the calcified chondrocyte marker osteopontin, without markedly affecting that of a proliferating chondrocyte marker or a hypertrophic chondrocyte marker, as shown in organotypic cultures by in situ hybridization analysis. Gly significantly increased Ca2+ accumulation, osteopontin mRNA expression, and alkaline phosphatase activity in cultured rat costal chondrocytes, without significantly affecting those in cultured rat calvarial osteoblasts. The increase induced by Gly was significantly prevented by an NMDA receptor channel blocker and an antagonist at the Gly site on NMDA receptors, but not by an inhibitory Gly receptor antagonist or a Gly transporter inhibitor, in cultured chondrocytes. Constitutive mRNA expression was seen for NR1, NR2D, and NR3A subunits of NMDA receptors, but not for Gly receptors and transporters, in cultured chondrocytes. Corresponding immunoreactive proteins were detected for NR1 and NR2D subunits in cartilaginous zones of fetal mouse tibias. Thus, Gly might, at least in part, play a role as a trophic factor in the mechanisms associated with chondral calcification through the Gly site of NMDA receptors functionally expressed by chondrocytes in rodent cartilage.

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Crossref · 10.1007/s00441-008-0607-7

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  • NMDA receptors have been implicated in the maturation and differentiation of chondrocytes.

    “NMDA receptors have been implicated in the maturation and differentiation of chondrocytes (Takahata et al., 2008).”

    From Glutamate receptors in domestication and modern human evolution (2020) · cites this paper as Takahata et al., 2008

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Authors
Takahata, Y., Takarada, T., Osawa, M., Hinoi, E., Nakamura, Y., & Yoneda, Y.
Affiliations
Kanazawa University (JP)
Corresponding author
Yoneda, Yukio
Venue
Cell and Tissue Research
Published
2008-04-22
Volume
333
Issue
1
Pages
91-103
ISSN
  • 0302-766X (ISSN-L)
  • 1432-0878
OpenAlex
W2088366348
Cited by
22
References
46
Citations by year
2024: 1 · 2022: 1 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 3 · 2016: 1 · 2014: 1 · 2013: 1 · 2012: 3
FWCI
0.92
Type
journal article
Language
en
Open access
closed
Locations
  • Cell and Tissue Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
Keywords
glycine, NMDA receptor, chondrocytes, osteoblasts, chondrogenesis, osteopontin, NMDA receptor subunits, trophic factors, organotypic culture, cartilage, alkaline phosphatase, calcium accumulation
MeSH terms
Alkaline Phosphatase, Animals, Calcification, Physiologic, Cells, Cultured, Embryo, Mammalian, Female, Glycine, Immunohistochemistry, Mice, Inbred Strains, Organ Culture Techniques, Osteoblasts, Ribs, Skull, Tibia, Rats, Wistar, In Situ Hybridization, Chondrocytes, Chondrogenesis, Mice, Rats, Osteopontin

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