Group III metabotropic glutamate receptors in rat cultured calvarial osteoblasts
Abstract
Reverse transcription polymerase chain reaction revealed expression of mRNAs for particular receptors for the central neurotransmitter l-glutamic acid (Glu) in primary cultures of rat calvarial osteoblastic cells under premature to mature states according to the duration of days in vitro. These included metabotropic Glu receptors (mGluR) such as mGluR4 and mGluR8, in addition to several ionotropic Glu receptor subunits including NR1 and NR2D. Expression of mRNAs was not detected with other mGluR and NR2A-C subunits irrespective of the maturity of cultured cells. The agonist for group III mGluR L-(+)-2-amino-4-phosphonobutyric acid significantly inhibited the forskolin-induced accumulation of cAMP in premature osteoblasts, which occurred in a manner sensitive to prevention by the group III mGluR antagonist (RS)-alpha-cyclopropyl-4-phosphonophenylglycine. These results suggest that Glu may at least in part play a role in mechanisms associated with cellular proliferation and/or differentiation through group III mGluR functionally expressed in rat calvarial osteoblastic cells.
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GRIK5, GRM4, and GRM8 along with NMDA and AMPA receptor genes are expressed in vitro in developing rat calvarial osteoblasts.
“GRIK5, GRM4, and GRM8 along with NMDA and AMPA receptor genes are expressed in vitro in developing rat calvarial osteoblasts (Hinoi et al., 2001(Hinoi et al., , 2002)).”
From Glutamate receptors in domestication and modern human evolution (2020) · cites this paper as Hinoi et al., 2001
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- Authors
- Hinoi, E., Fujimori, S., Nakamura, Y., & Yoneda, Y.
- Affiliations
- Kanazawa University (JP)
- Corresponding author
- Yoneda, Yukio
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2001-02
- Volume
- 281
- Issue
- 2
- Pages
- 341-346
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2014577644
- Cited by
- 80
- References
- 38
- Citations by year
- 2022: 2 · 2021: 1 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2012: 4
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- group III metabotropic glutamate receptors, mGluR4, mGluR8, calvarial osteoblasts, osteogenic differentiation, glutamate, ionotropic glutamate receptors, NR1, NR2D, cAMP signaling, forskolin, BAPTA, osteoblast proliferation, gene expression
- MeSH terms
- Cyclic AMP, Aminobutyrates, Animals, Animals, Newborn, Cells, Cultured, Colforsin, Glycine, Male, Osteoblasts, RNA, Messenger, Skull, 1-Methyl-3-isobutylxanthine, Gene Expression, Receptors, N-Methyl-D-Aspartate, Rats, Wistar, Receptors, Metabotropic Glutamate, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Rats
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