Distribution of microglia in the postnatal murine nigrostriatal system
Abstract
Parkinson's disease (PD) is characterized by the degeneration of dopaminergic neurons in the substantia nigra (SN) and the subsequent loss of striatal target innervation. Neuroinflammatory responses have been described for virtually all PD cases analysed. Microglia are the resident immune cells of the central nervous system and, thus, are the mediators of neuroinflammation. Approximately 12% of all central nervous system cells are microglia but the distribution and density of microglia differ within distinct brain regions. Interestingly, the SN has been shown to contain more microglia than adjacent structures. We have analysed changes in microglia numbers and in microglial morphology in the postnatal murine nigrostriatal system at various stages ranging from postnatal day 0 (P0) up to 24 months of age. We clearly show that the microglia numbers in the SN and in the striatum dramatically increase from P0 to P15 and significantly decrease in both areas in 18-month-old and 24-month-old animals. Moreover, microglia in the nigrostriatal system of aged mice show signs of dystrophy and degeneration, such as cytoplasmic inclusions, deramification of their processes and membrane blebbing. Our results support the hypothesis of microglial dystrophy during aging in the murine nigrostriatal system, accompanied by subsequent impairment of normal microglial functions. Microglial dysfunction during aging might be a potential risk factor for the development and/or progression of PD.
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Previous immunohistochemical studies of microglia did not distinguish between SNc and SNr.
“previous immunohistochemical studies of microglia did not distinguish between SNc/SNr (Lawson et al., 1990; Sharaf et al., 2013; Yang et al., 2013)”
From Local cues establish and maintain region-specific phenotypes of basal ganglia microglia (2017) · cites this paper as Sharaf et al., 2013
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- Authors
- Sharaf, A., Krieglstein, K., & Spittau, B.
- Affiliations
- University of Freiburg (DE)
- Corresponding author
- Spittau, Björn
- Venue
- Cell and Tissue Research
- Published
- 2012-12-19
- Volume
- 351
- Issue
- 3
- Pages
- 373-382
- ISSN
- 0302-766X (ISSN-L)
- 1432-0878
- OpenAlex
- W2028545133
- Cited by
- 59
- References
- 32
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 7 · 2020: 7 · 2019: 7 · 2018: 7 · 2017: 8 · 2016: 2 · 2015: 2 · 2013: 2
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell and Tissue Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Higher Education and Scientific Research
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- microglia, nigrostriatal pathway, substantia nigra, striatum, Parkinson's disease, microglial morphology, microglial density, aging, neuroinflammation, dopaminergic neurons, postnatal development, microglial dysfunction
- MeSH terms
- Aging, Animals, Animals, Newborn, Calcium-Binding Proteins, Cell Count, Fluorescent Antibody Technique, Male, Mice, Inbred C57BL, Microfilament Proteins, Substantia Nigra, Microglia, Proliferating Cell Nuclear Antigen, Cell Shape, Cell Proliferation, Mice
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