Cited by 11Multiple Sclerosis Research StudiesT-cell and B-cell ImmunologyPsoriasis: Treatment and Pathogenesis

Analysis of LAP+ and GARP+ Treg subsets in peripheral blood of patients with neuromyelitis optica spectrum disorders

Cai, Haobing, Liu, Yu, Dong, Xiaohua, Jiang, Fei, Li, Hongliang, Ouyang, Song, Yin, Weifan, He, Ting +2 more·2023·Neurological Sciences

Abstract

Introduction Neuromyelitis optica spectrum disorder (NMOSD) is a group of antibody-mediated inflammatory demyelinating central nervous system diseases. T lymphocytes participate in NMOSD pathogenesis, with regulatory T cells (Treg) being the core in maintaining immune homeostasis. Studies have revealed that different Treg subsets play different roles in autoimmune diseases. The distribution of LAP+ or GARP+ Treg subsets in NMOSD may help us deeply understand their immune mechanism. Methods This study reviewed 22 NMOSD patients and 20 normal controls. Flow cytometric analysis was utilized to detect subsets of Treg cells expressing Foxp3, Helios, LAP, or GARP in peripheral blood. ELISA was used to detect plasma TGF-β1 and IL-10. In addition, changes in the proportion of Treg cell subsets before and after glucocorticoid treatment in 10 patients were analyzed. Results Compared with healthy controls, LAP and GARP expressions were significantly downregulated in the peripheral blood of NMOSD patients. TGF-β1 expression in NMOSD patients was lower and was positively correlated with the ratio of CD4+GARP+ Treg cells. After treatment with glucocorticoid, LAP and GARP expressions in the peripheral blood of NMOSD patients were upregulated. Conclusions The proportion of Treg cells expressing LAP and GARP is downregulated, implying that Treg cells with the best inhibitory function are insufficient to maintain autoimmune homeostasis in NMOSD patients. Upregulation of Treg cells expressing LAP and GARP in NMOSD patients may be one of the mechanisms of glucocorticoid treatment.

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Crossref · 10.1007/s10072-023-06629-8

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Authors
Cai, H., Liu, Y., Dong, X., Jiang, F., Li, H., Ouyang, S., Yin, W., He, T., Zeng, Q., & Yang, H.
Affiliations
  • Central South University (CN)
  • Second People Hospital of Hunan (CN)
  • Hunan University of Traditional Chinese Medicine (CN)
  • The First Hospital of Changsha (CN)
Corresponding author
  • Zeng, Qiuming
  • Yang, Huan
Venue
Neurological Sciences
Published
2023-01-23
Volume
44
Issue
5
Pages
1739-1747
ISSN
  • 1590-1874 (ISSN-L)
  • 1590-3478
OpenAlex
W4317715931
Cited by
11
References
35
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 1
FWCI
2.19
Type
journal article
Language
en
Open access
closed
Locations
  • Neurological Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81801203)
  • Hunan Academician Expert Workstation (Shi Xuemin) Open-end Fund Project (2019YSZJJ12)
  • Natural Science Foundation of Hunan Province (2022JJ40724)
  • Science and Technology Innovation Guidance Project of Hunan Province (2020SK53009)
  • Changsha Municipal Natural Science Foundation (kq2007037)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
Keywords
neuromyelitis optica spectrum disorder, regulatory T cells, Foxp3, Helios, TGF-β1, IL-10, glucocorticoid therapy, demyelinating disease, immune homeostasis, CD4+ T cells
MeSH terms
Glucocorticoids, Humans, Membrane Proteins, Neuromyelitis Optica, T-Lymphocytes, Regulatory, Transforming Growth Factor beta1

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