Synthetic Materials in the Study of Cell Response to Substrate Rigidity
Abstract
While it has long been understood that cells can sense and respond to a variety of stimuli, including soluble and insoluble factors, light, and externally applied mechanical stresses, the extent to which cells can sense and respond to the mechanical properties of their environment has only recently begun to be studied. Cell response to substrate stiffness has been suggested to play an important role in processes ranging from developmental morphogenesis to the pathogenesis of disease states and may have profound implications for cell and tissue culture and tissue engineering. Given the importance of this phenomenon, there is a clear need for systems for cell study in which substrate mechanics can be carefully defined and varied independently of biochemical and other signals. This review will highlight past work in the field of cell response to substrate rigidity as well as areas for future study.
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The mechanical properties of Matrigel-based culture systems cannot be easily separated from their chemical cues.
“The mechanical properties, such as elastic modulus, pore size, stress relaxation, and creep56–59, cannot be easily separated from the chemical cues in the Matrigel-based culture systems.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [58]
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- Authors
- Nemir, S., & West, J. L.
- Affiliations
- Rice University (US)
- Corresponding author
- West, Jennifer L.
- Venue
- Annals of Biomedical Engineering
- Published
- 2009-10-09
- Volume
- 38
- Issue
- 1
- Pages
- 2-20
- ISSN
- 0090-6964 (ISSN-L)
- 1573-9686
- OpenAlex
- W2060566320
- Cited by
- 318
- References
- 83
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 16 · 2023: 14 · 2022: 12 · 2021: 14 · 2020: 16 · 2019: 19 · 2018: 16 · 2017: 23 · 2016: 15 · 2015: 21 · 2014: 24 · 2013: 36 · 2012: 35
- FWCI
- 12.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Annals of Biomedical Engineering (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- substrate stiffness, mechanotransduction, artificial substrates, cell mechanics, tissue engineering, cell culture substrates, mechanobiology
- MeSH terms
- Animals, Biocompatible Materials, Cells, Cultured, Humans, Materials Testing
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