The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Lipopolysaccharide-activated microglia induce dysfunction of the blood-brain barrier in rat microvascular endothelial cells co-cultured with microglia
Open accessAbstract
The blood-brain barrier (BBB) is formed by brain capillary endothelial cells, astrocytes, pericytes, microglia, and neurons. BBB disruption under pathological conditions such as neurodegenerative disease and inflammation is observed in parallel with microglial activation. To test whether activation of microglia is linked to BBB dysfunction, we evaluated the effect of lipopolysaccharide (LPS) on BBB functions in an in vitro co-culture system with rat brain microvascular endothelial cells (RBEC) and microglia. When LPS was added for 6 h to the abluminal side of RBEC/microglia co-culture at a concentration showing no effects on the RBEC monolayer, transendothelial electrical resistance was decreased and permeability to sodium-fluorescein was increased in RBEC. Immunofluorescence staining for tight junction proteins demonstrated that zonula occludens-1-, claudin-5-, and occludin-like immunoreactivities at the intercellular borders of RBEC were fragmented in the presence of LPS-activated microglia. These functional changes induced by LPS-activated microglia were blocked by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, diphenyleneiodonium chloride. The present findings suggest that LPS activates microglia to induce dysfunction of the BBB by producing reactive oxygen species through NADPH oxidase.
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“Recent studies suggest that microglial activation may be related to BBB disruption.”
From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Sumi et al., 2010
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Toll-like Receptor-4 Mediates Lipopolysaccharide-induced Signal Transduction
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Metadata
- Authors
- Sumi, N., Nishioku, T., Takata, F., Matsumoto, J., Watanabe, T., Shuto, H., Yamauchi, A., Dohgu, S., & Kataoka, Y.
- Affiliations
- Fukuoka University (JP)
- Corresponding author
- Kataoka, Yasufumi
- ORCID
- Venue
- Cellular and Molecular Neurobiology
- Published
- 2009-08-29
- Volume
- 30
- Issue
- 2
- Pages
- 247-253
- ISSN
- 0272-4340 (ISSN-L)
- 1573-6830
- PMC
- PMC11498813
- OpenAlex
- W1990985572
- Cited by
- 179
- References
- 33
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 16 · 2023: 11 · 2022: 13 · 2021: 20 · 2020: 14 · 2019: 13 · 2018: 14 · 2017: 12 · 2016: 12 · 2015: 11 · 2014: 8 · 2013: 5 · 2012: 5
- FWCI
- 3.41
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cellular and Molecular Neurobiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- blood-brain barrier dysfunction, microglial activation, lipopolysaccharide, brain microvascular endothelial cells, endothelial permeability, tight junction proteins, zonula occludens-1, claudin-5, occludin, NADPH oxidase, reactive oxygen species, diphenyleneiodonium, transendothelial electrical resistance, co-culture model
- MeSH terms
- Animals, Blood-Brain Barrier, Cell Membrane Permeability, Lipopolysaccharides, Rats, Wistar, Reactive Oxygen Species, Microglia, Coculture Techniques, Endothelial Cells, Rats
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