Cited by 139Wound Healing and TreatmentsExtracellular vesicles in diseaseElectrospun Nanofibers in Biomedical Applications

GelMA combined with sustained release of HUVECs derived exosomes for promoting cutaneous wound healing and facilitating skin regeneration

Zhao, Danyang, Yu, Zhencheng, Li, Yun, Wang, Yu, Li, Qingfeng, Han, Dong·2020·Journal of Molecular Histology

Abstract

It remains a clinical challenge for cutaneous wound healing and skin regeneration. Endothelial cells participate in the formation of blood vessels and play an important role in the whole process of wound healing. Recent studies suggested that exosomes contribute to the intercellular communication through paracrine pathways, and sustained release of exosomes from hydrogel-based materials provide a promising strategy for curing wound defects. In this study, we isolated exosomes derived from human umbilical vein endothelial cells (HUVECs) and found that HUVECs derived exosomes (HUVECs-Exos) could promote the proliferation and migration activities of keratinocytes and fibroblasts, which are two important effector cells for skin regeneration. Then we developed gelatin methacryloyl (GelMA) hydrogel as the wound dressing to incorporate HUVECs-Exos and applied it to the full-thickness cutaneous wounds. It demonstrated that GelMA scaffold could not only repair the wound defect, but also achieve sustained release of exosomes. The in vivo results showed accelerated re-epithelialization, promotion of collagen maturity and improvement of angiogenesis. Collectively, our findings suggested that HUVECs-Exos could accelerate wound healing and GelMA mediated controlled release of HUVECs-Exos might offer a new method for repairing cutaneous wound defects.

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Registry

Crossref · 10.1007/s10735-020-09877-6

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67 of 67 references verified0 citation errors · 0 not checkable

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  • HUVEC-derived exosomes can promote collagen maturation and angiogenesis and regulate keratinocytes and fibroblast growth, thereby accelerating skin wound healing.

    “For example, exosomes derived from human umbilical vein endothelial cells (HUVECs) can promote collagen maturation and angiogenesis and regulate the keratinocytes and fibroblast growth, thereby accelerating skin wound healing [102].”

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Authors
Zhao, D., Yu, Z., Li, Y., Wang, Y., Li, Q., & Han, D.
Affiliations
Shanghai Jiao Tong University (CN)
Corresponding author
  • Li, Qingfeng
  • Han, Dong
Venue
Journal of Molecular Histology
Published
2020-05-09
Volume
51
Issue
3
Pages
251-263
ISSN
  • 1567-2379 (ISSN-L)
  • 1567-2387
OpenAlex
W3023186039
Cited by
139
References
67
Citations by year
2026: 15 · 2025: 21 · 2024: 27 · 2023: 27 · 2022: 31 · 2021: 16 · 2020: 2
FWCI
10.53
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Molecular Histology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (81620108019, 81571944)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Keywords
exosomes, human umbilical vein endothelial cells, gelatin methacryloyl hydrogel, skin wound healing, skin regeneration, wound dressings, keratinocyte proliferation and migration, fibroblast proliferation and migration, re-epithelialization, collagen maturation, angiogenesis, paracrine signaling
MeSH terms
Animals, Cell Movement, Collagen, Fibroblasts, Humans, Male, Regeneration, Skin, Wound Healing, Keratinocytes, Rats, Sprague-Dawley, Neovascularization, Physiologic, Hydrogels, Cell Proliferation, Exosomes, Human Umbilical Vein Endothelial Cells, In Vitro Techniques

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