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GelMA combined with sustained release of HUVECs derived exosomes for promoting cutaneous wound healing and facilitating skin regeneration
Abstract
It remains a clinical challenge for cutaneous wound healing and skin regeneration. Endothelial cells participate in the formation of blood vessels and play an important role in the whole process of wound healing. Recent studies suggested that exosomes contribute to the intercellular communication through paracrine pathways, and sustained release of exosomes from hydrogel-based materials provide a promising strategy for curing wound defects. In this study, we isolated exosomes derived from human umbilical vein endothelial cells (HUVECs) and found that HUVECs derived exosomes (HUVECs-Exos) could promote the proliferation and migration activities of keratinocytes and fibroblasts, which are two important effector cells for skin regeneration. Then we developed gelatin methacryloyl (GelMA) hydrogel as the wound dressing to incorporate HUVECs-Exos and applied it to the full-thickness cutaneous wounds. It demonstrated that GelMA scaffold could not only repair the wound defect, but also achieve sustained release of exosomes. The in vivo results showed accelerated re-epithelialization, promotion of collagen maturity and improvement of angiogenesis. Collectively, our findings suggested that HUVECs-Exos could accelerate wound healing and GelMA mediated controlled release of HUVECs-Exos might offer a new method for repairing cutaneous wound defects.
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“For example, exosomes derived from human umbilical vein endothelial cells (HUVECs) can promote collagen maturation and angiogenesis and regulate the keratinocytes and fibroblast growth, thereby accelerating skin wound healing [102].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [102]
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Metadata
- Authors
- Zhao, D., Yu, Z., Li, Y., Wang, Y., Li, Q., & Han, D.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Li, Qingfeng
- Han, Dong
- Venue
- Journal of Molecular Histology
- Published
- 2020-05-09
- Volume
- 51
- Issue
- 3
- Pages
- 251-263
- ISSN
- 1567-2379 (ISSN-L)
- 1567-2387
- OpenAlex
- W3023186039
- Cited by
- 139
- References
- 67
- Citations by year
- 2026: 15 · 2025: 21 · 2024: 27 · 2023: 27 · 2022: 31 · 2021: 16 · 2020: 2
- FWCI
- 10.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Histology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81620108019, 81571944)
- Topics
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- exosomes, human umbilical vein endothelial cells, gelatin methacryloyl hydrogel, skin wound healing, skin regeneration, wound dressings, keratinocyte proliferation and migration, fibroblast proliferation and migration, re-epithelialization, collagen maturation, angiogenesis, paracrine signaling
- MeSH terms
- Animals, Cell Movement, Collagen, Fibroblasts, Humans, Male, Regeneration, Skin, Wound Healing, Keratinocytes, Rats, Sprague-Dawley, Neovascularization, Physiologic, Hydrogels, Cell Proliferation, Exosomes, Human Umbilical Vein Endothelial Cells, In Vitro Techniques
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