CoChat
Sign Up
Cited by 254Immune Response and InflammationImmune cells in cancerImmune responses and vaccinations

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Impaired Functions of Peripheral Blood Monocyte Subpopulations in Aged Humans

Open access
Nyugen, Joseph, Agrawal, Sudhanshu, Gollapudi, Sastry, Gupta, Sudhir·2010·Journal of Clinical Immunology
DOI 10.1007/s10875-010-9448-8PMC PMC2970801
Open this paper in CoChat — free accountOpen PDF

Abstract

Aging is associated with increased susceptibility to microbial infections, and monocytes play an important role in microbial defense. In this study, we have identified and compared four subpopulations of monocytes (CD14(++(high))CD16(-), CD14(+(low))CD16(-), CD14(++(high))CD16(+), and CD14(+(low))CD16(+)) in the peripheral blood of young and aged subjects with regard to their numbers, cytokine production, TLR expression, and phosphorylation of ERK1/2 in response to pam3Cys a TLR-1/2 ligand. Proportions and numbers of CD14(++(high))CD16(+) and CD14(+(low))CD16(+) monocytes were significantly increased, whereas proportions of CD14(+(low))CD16(-) monocytes were decreased in aged subjects as compared to young subjects. In aged subjects, IL-6 production by all four subsets of monocytes was significantly decreased, whereas TNF-α production was decreased in monocyte subsets, except the CD14(+(low))CD16(-) subset. A significantly reduced expression of TLR1 was observed in CD14(++(high))CD16(+) and CD14(+(low))CD16(+) monocyte subsets in aged subjects. Furthermore, following pam3Cys stimulation, ERK1/2 phosphorylation was significantly lower in CD14(+(low))CD16(+), CD14(++(high))CD16(+), and CD14(+(low))CD16(-) subsets of monocytes from aged subjects. This is the first study of four subpopulations of monocytes in aging, which demonstrates that their functions are differentially impaired with regard to the production of cytokines, expression of TLR, and signaling via the ERK-MAPK pathway. Finally, changes in the number of monocyte subsets, and impairment of TLR1 expression, TNF-α production, and EK1/2 phosphorylation was more consistent in CD16(+) monocyte subsets regardless of expression of CD14(high) or CD14(+low), therefore highlighting the significance of further subdivision of monocytes into four subpopulations.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s10875-010-9448-8

Cites

31 of 31 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

See all 254

Related

See all

Metadata

Authors
Nyugen, J., Agrawal, S., Gollapudi, S., & Gupta, S.
Affiliations
University of California, Irvine (US)
Corresponding author
Gupta, Sudhir
ORCID
Gupta, Sudhir
Venue
Journal of Clinical Immunology
Publisher
Springer Science and Business Media LLC
Published
2010-08-12
Volume
30
Issue
6
Pages
806-813
ISSN
  • 0271-9142 (ISSN-L)
  • 1573-2592
DOI
10.1007/s10875-010-9448-8
PMC
PMC2970801
OpenAlex
W2015163231
Cited by
249
References
31
Citations by year
2026: 6 · 2025: 22 · 2024: 19 · 2023: 17 · 2022: 13 · 2021: 32 · 2020: 18 · 2019: 13 · 2018: 12 · 2017: 15 · 2016: 11 · 2015: 12 · 2014: 19 · 2013: 20 · 2012: 13
FWCI
5.09
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Journal of Clinical Immunology (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • eScholarship (California Digital Library) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
Keywords
monocyte subpopulations, aging, CD14, CD16, TLR1, ERK1/2 phosphorylation, MAPK signaling, interleukin-6, TNF-α, cytokine production, Pam3Cys, immunosenescence
MeSH terms
Adult, Aged, Aged, 80 and over, Aging, Bacterial Infections, Cell Differentiation, Cells, Cultured, Gene Expression Regulation, Humans, Macrophages, Signal Transduction, Antigens, CD, Interleukin-6, Mitogen-Activated Protein Kinase 3, Toll-Like Receptors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review