Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus
Abstract
Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 microM), sodium nitroprusside (5 microM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1'-1' Diphenyl-2' picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.
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Iron increases lipid peroxidation in tissue homogenates, which can be prevented by antioxidants.
“The rationale for these experiments is that iron increases lipid peroxidation in the tissue homogenates, which can be prevented by antioxidants [41].”
From Sodium nitrite downregulates vascular NADPH oxidase and exerts antihypertensive effects in hypertension (2011) · cites this paper as [41]
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- Authors
- Pereira, R. P., Fachinetto, R., de Souza Prestes, A., Puntel, R. L., Santos da Silva, G. N., Heinzmann, B. M., Boschetti, T. K., Athayde, M. L., Bürger, M. E., Morel, A. F., Morsch, V. M., & Rocha, J. B. T.
- Affiliations
- Universidade Federal de Santa Maria (BR)
- The Abdus Salam International Centre for Theoretical Physics (ICTP) (IT)
- Corresponding author
- Rocha, João Batista Teixeira
- ORCID
- Venue
- Neurochemical Research
- Published
- 2008-10-14
- Volume
- 34
- Issue
- 5
- Pages
- 973-983
- ISSN
- 0364-3190 (ISSN-L)
- 1573-6903
- OpenAlex
- W1977780735
- Cited by
- 226
- References
- 65
- Citations by year
- 2026: 10 · 2025: 7 · 2024: 7 · 2023: 13 · 2022: 12 · 2021: 16 · 2020: 15 · 2019: 10 · 2018: 17 · 2017: 9 · 2016: 20 · 2015: 16 · 2014: 13 · 2013: 17 · 2012: 19
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Neurochemical Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Abdus Salam International Centre for Theoretical Physics
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Financiadora de Estudos e Projetos
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry Medicinal Plant Applications
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- lemon balm, chamomile, Cymbopogon citratus, antioxidant activity, lipid peroxidation, oxidative stress, neurological disorders, quercetin, gallic acid, quercitrin, rutin, phenolic compounds, plant extracts, free radical scavenging, 3-nitropropionic acid, sodium nitroprusside, iron sulfate
- MeSH terms
- Animals, Antioxidants, Brain, Ferrous Compounds, Gallic Acid, Male, Nitro Compounds, Nitroprusside, Phenols, Plant Extracts, Propionates, Quercetin, Rutin, Free Radical Scavengers, Rats, Wistar, Thiobarbituric Acid Reactive Substances, Oxidative Stress, Melissa, Cymbopogon, Rats, In Vitro Techniques
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