Enhanced production of lutein in heterotrophic Chlorella protothecoides by oxidative stress
Abstract
The fast growing unicellular green microalgae Chlorella protothecoides has attracted interest as a promising organism for commercial production of a high-value carotenoid, lutein, by heterotrophic fermentation. Effects of two oxidant-forming reactive oxygen species (ROS) on the biomass concentration, and yield and content of lutein in batch culture of heterotrophic Chlorella protothecoides were investigated in this study. The addition of 0.1 mmol/L H(2)O(2) and 0.01 mmol/L NaClO plus 0.5 mmol/L Fe(2+) to the culture led to the generation of (.)OH and enhanced the lutein content from 1.75 to 1.90 and 1.95 mg/g, respectively. The lutein content further increased to 1.98 mg/g when 0.01 mmol/L H(2)O(2) and 0.5 mmol/L NaClO were added to generate (1)O(2). The maximum yield of lutein (28.5, 29.8 and 31.4 mg/L) and a high biomass concentration (15.0, 15.3 and 15.9 g/L) were also achieved through the above treatments. The results indicated that 1O2 could promote lutein formation and enhance lutein production in heterotrophic Chlorella protothecoides. Moreover, (1)O(2) produced from the reaction of H(2)O(2) and NaClO was more effective in enhancing lutein production and reducing biomass loss than (.)OH from the reaction of H(2)O(2) or NaClO plus Fe(2+).
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Lutein from Chlorella protothecoides in batch culture under specified conditions produced lutein 10 mgL−1 d−1.
“| Lutein | Chlorella protothecoides | Batch | Temperature; pH | Temperature: 28 °C; pH: 6.5; Light Intensity: absence of light; Metabolic mode: heterotrophic | Lutein: 10 mgL−1 d−1 | Wei et al. (2008) |”
From Production of microalgae with high lipid content and their potential as sources of nutraceuticals (2023) · cites this paper as Wei et al. (2008)
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- Authors
- Wei, D., Chen, F., Chen, G., Zhang, X., Liu, L., & Zhang, H.
- Affiliations
- South China University of Technology (CN)
- University of Hong Kong (HK)
- Jiangnan University (CN)
- Corresponding author
- Wei, Dong
- Venue
- Science in China Series C: Life Sciences
- Published
- 2008-12
- Volume
- 51
- Issue
- 12
- Pages
- 1088-1093
- ISSN
- 1006-9305 (ISSN-L)
- 1862-2798
- OpenAlex
- W2021013048
- Cited by
- 91
- References
- 35
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 9 · 2023: 6 · 2022: 6 · 2021: 5 · 2020: 7 · 2019: 6 · 2018: 10 · 2017: 7 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 5 · 2012: 4
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Science in China Series C Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- lutein production, Chlorella protothecoides, heterotrophic fermentation, oxidative stress, reactive oxygen species, singlet oxygen, hydrogen peroxide, sodium hypochlorite, hydroxyl radicals, carotenoid biosynthesis, biomass concentration
- MeSH terms
- Chlorella, Hydrogen Peroxide, Sodium Hypochlorite, Lutein, Molecular Structure, Oxidants, Reactive Oxygen Species, Oxidative Stress, Algal Proteins
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