Cited by 177Protein Hydrolysis and Bioactive PeptidesSeaweed-derived Bioactive CompoundsRenin-Angiotensin System Studies

Isolation and Characterization of Bioactive Pro-Peptides with in Vitro Renin Inhibitory Activities from the Macroalga Palmaria palmata

Fitzgerald, Ciarán, Mora-Soler, Leticia, Gallagher, Eimear, O’Connor, Paula, Prieto, Jose, Soler-Vila, Anna, Hayes, Maria·2012·Journal of Agricultural and Food Chemistry

Abstract

Renin is the initial rate limiting step in the renin angiotensinogen system (RAS). To combat hypertension, various stages of the RAS can be positively affected. The aim of this study was to isolate and characterize renin inhibitory peptides from the red seaweed P. palmata for use in functional foods. Palmaria palmata protein was extracted and hydrolyzed with the food grade enzyme Papain to generate renin inhibitory peptides. Following proteolytic hydrolysis of P. palmata protein, reverse phase-high performance liquid chromatography (RP-HPLC) was employed to enrich for peptides with renin inhibitory activities. Fraction 25 (Fr-25) inhibited renin activities by 58.97% (±1.26) at a concentration of 1 mg/mL. This fraction was further characterized using nano-electrospray ionization quadropole/time-of-flight mass spectrometry (ESI-Q/TOF MS). A number of novel peptide sequences were elucidated, and the parent protein from which they were derived was determined using MS in tandem with protein database searches. All sequences were confirmed using de novo sequencing. The renin inhibitory peptide Ile-Arg-Leu-Ile-Ile-Val-Leu-Met-Pro-Ile-Leu-Met-Ala (IRLIIVLMPILMA) was chemically synthesized and its bioactivity confirmed using the renin inhibitory assay. Other stages of the RAS have recently been inhibited by bioactive peptides sourced from macroalgae, but this is the first study to isolate and characterize renin inhibitory peptides from the macroalgae.

Integrity signals

Registry

Crossref · 10.1021/jf301361c

Cites

23 of 23 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Seaweed-derived peptides from Undaria pinnatifida, Neopyropia yezoensis, Ulva rigida, and Ulva clathrata have shown ACE inhibitory activity.

    “Among seaweed-derived peptides, species such as Undaria pinnatifida (Phaeophyceae), Neopyropia yezoensis (formerly Porphyra yezoensis) (Rhodophyta), Ulva rigida, and Ulva clathrata (formerly Enteromorpha clathrata) have shown ACE inhibitory activity [53–57].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Fitzgerald, C., Mora-Soler, L., Gallagher, E., O’Connor, P., Prieto, J., Soler-Vila, A., & Hayes, M.
Affiliations
  • Teagasc - The Irish Agriculture and Food Development Authority (IE)
  • Food Biosciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland
  • Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, U.K.
  • Irish Seaweed Research Group, Ryan Institute, Environmental, Marine and Energy Research, National University of Ireland, Galway, Ireland
Venue
Journal of Agricultural and Food Chemistry
Published
2012-07-19
Volume
60
Issue
30
Pages
7421-7427
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2058334127
Cited by
177
References
23
Citations by year
2026: 6 · 2025: 12 · 2024: 19 · 2023: 12 · 2022: 16 · 2021: 14 · 2020: 11 · 2019: 16 · 2018: 11 · 2017: 16 · 2016: 12 · 2015: 14 · 2014: 8 · 2013: 10
FWCI
4.11
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
Keywords
propeptide, Palmaria palmata, red macroalgae, renin-angiotensin system, hypertension, papain hydrolysis, peptide isolation, de novo sequencing, synthetic peptides, functional foods, seaweed proteins
MeSH terms
Renin Inhibitors, Rhodophyta, Angiotensinogen, Chromatography, High Pressure Liquid, Oligopeptides, Papain, Plant Extracts, Renin, Seaweed, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review