Isolation and Characterization of Bioactive Pro-Peptides with in Vitro Renin Inhibitory Activities from the Macroalga Palmaria palmata
Abstract
Renin is the initial rate limiting step in the renin angiotensinogen system (RAS). To combat hypertension, various stages of the RAS can be positively affected. The aim of this study was to isolate and characterize renin inhibitory peptides from the red seaweed P. palmata for use in functional foods. Palmaria palmata protein was extracted and hydrolyzed with the food grade enzyme Papain to generate renin inhibitory peptides. Following proteolytic hydrolysis of P. palmata protein, reverse phase-high performance liquid chromatography (RP-HPLC) was employed to enrich for peptides with renin inhibitory activities. Fraction 25 (Fr-25) inhibited renin activities by 58.97% (±1.26) at a concentration of 1 mg/mL. This fraction was further characterized using nano-electrospray ionization quadropole/time-of-flight mass spectrometry (ESI-Q/TOF MS). A number of novel peptide sequences were elucidated, and the parent protein from which they were derived was determined using MS in tandem with protein database searches. All sequences were confirmed using de novo sequencing. The renin inhibitory peptide Ile-Arg-Leu-Ile-Ile-Val-Leu-Met-Pro-Ile-Leu-Met-Ala (IRLIIVLMPILMA) was chemically synthesized and its bioactivity confirmed using the renin inhibitory assay. Other stages of the RAS have recently been inhibited by bioactive peptides sourced from macroalgae, but this is the first study to isolate and characterize renin inhibitory peptides from the macroalgae.
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Seaweed-derived peptides from Undaria pinnatifida, Neopyropia yezoensis, Ulva rigida, and Ulva clathrata have shown ACE inhibitory activity.
“Among seaweed-derived peptides, species such as Undaria pinnatifida (Phaeophyceae), Neopyropia yezoensis (formerly Porphyra yezoensis) (Rhodophyta), Ulva rigida, and Ulva clathrata (formerly Enteromorpha clathrata) have shown ACE inhibitory activity [53–57].”
From Navigating the potential of algal peptides: health effects, market applications, and scientific challenges (2026) · cites this paper as [55]
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Self-citation
- Authors
- Fitzgerald, C., Mora-Soler, L., Gallagher, E., O’Connor, P., Prieto, J., Soler-Vila, A., & Hayes, M.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Food Biosciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland
- Department of Pharmaceutical and Biological Chemistry, School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX, U.K.
- Irish Seaweed Research Group, Ryan Institute, Environmental, Marine and Energy Research, National University of Ireland, Galway, Ireland
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-07-19
- Volume
- 60
- Issue
- 30
- Pages
- 7421-7427
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2058334127
- Cited by
- 177
- References
- 23
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 19 · 2023: 12 · 2022: 16 · 2021: 14 · 2020: 11 · 2019: 16 · 2018: 11 · 2017: 16 · 2016: 12 · 2015: 14 · 2014: 8 · 2013: 10
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
- Keywords
- propeptide, Palmaria palmata, red macroalgae, renin-angiotensin system, hypertension, papain hydrolysis, peptide isolation, de novo sequencing, synthetic peptides, functional foods, seaweed proteins
- MeSH terms
- Renin Inhibitors, Rhodophyta, Angiotensinogen, Chromatography, High Pressure Liquid, Oligopeptides, Papain, Plant Extracts, Renin, Seaweed, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
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