A proteomic investigation of B lymphocytes in an autistic family: a pilot study of exposure to natural rubber latex (NRL) may lead to autism
Abstract
Autism is a multi-factorial neurodevelopmental disorder. We have investigated the molecular mechanism involved in a Chinese family with autism by a proteomic approach. Antibody chips containing 500 spots of human protein antibodies were used to screen for differentially expressed proteins in the peripheral B lymphocytes between autistic and non-autistic siblings in this family. Four proteins relevant to immuno-pathway, including IKKα that was up-regulated and Tyk2, EIF4G1 and PRKCI that were down-regulated, were identified differentially expressed in autistic versus non-autistic siblings. Western blot analysis and reverse transcription quantitative polymerase chain reaction validated the differential expression of these four proteins. Based on the function of these differentially expressed proteins, relevant studies on immunoglobulin E (IgE) level, nuclear factor kappa B signaling activation and cell cycle were conducted in both autistic and non-autistic children of this family. Considering the fact that the family members were in close contact with natural rubber latex (NRL) and that IgE-mediated cross-reactions could be triggered by Hevea brasiliensis (Hev-b) proteins in NRL, we hypothesize that immune reactions triggered by close contact with NRL might influence the functions of B lymphocytes by altering expression of certain proteins identified in our experiments thus contributing to the occurrence of autism.
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PRKCI has been implied in immunosuppression, and genetic variation in the PRKCI gene has been found to be associated with bipolar disorder and autism.
“The Protein Kinase C Iota has been implied in immunosuppression (Sarkar et al., 2017), and genetic variation in the PRKCI gene has been found to be associated with bipolar disorder (Iwamoto et al., 2011) and autism (Shen et al., 2011).”
From Epigenome-wide DNA methylation in unipolar depression: predictive biomarker of antidepressant treatment response? (2024) · cites this paper as Shen et al., 2011
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- Authors
- Shen, C., Zhao, X.-L., Ju, W., Zou, X.-B., Huo, L.-R., Yan, W., Zou, J.-H., Yan, G.-D., Jenkins, E. C., Brown, W. T., & Zhong, N.
- Affiliations
- Peking University (CN)
- Shanxi Medical University (CN)
- Corresponding author
- Zhong, Nanbert
- Venue
- Journal of Molecular Neuroscience
- Published
- 2010-10-19
- Volume
- 43
- Issue
- 3
- Pages
- 443-452
- ISSN
- 0895-8696 (ISSN-L)
- 1559-1166
- OpenAlex
- W2161279233
- Cited by
- 18
- References
- 50
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 2 · 2020: 2 · 2019: 5 · 2017: 1 · 2014: 3 · 2012: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Molecular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office for People With Developmental Disabilities
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Social Sciences › Psychology › Clinical Psychology › Family and Disability Support Research
- Keywords
- autism spectrum disorder, B cells, natural rubber latex, Hevea brasiliensis, differentially expressed proteins, IKKα, TYK2, eIF4G1, PRKCI, NF-κB signaling, immunoglobulin E, family studies, peripheral blood B cells, cell cycle
- MeSH terms
- Animals, Antibodies, Autistic Disorder, B-Lymphocytes, Cell Cycle, Cell Line, Child, Child, Preschool, Female, Humans, Latex, Male, Pedigree, Pilot Projects, Rubber, Latex Hypersensitivity, Proteome, Hevea, Protein Array Analysis, Asian People
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