Asiaticoside Inhibits Neuronal Apoptosis and Promotes Functional Recovery After Spinal Cord Injury in Rats
Abstract
Spinal cord injury is a critical traumatic injury. This study aimed to investigate the possible protective effects of asiaticoside on neuronal injury in a rat model of spinal cord injury and to clarify the possible molecular mechanisms underlying these protective effects. Rats were randomly divided into the following four groups: naïve, sham, spinal cord injury (moderate mechanical compression injury model), and spinal cord injury + asiaticoside. Time to spontaneous urination after spinal cord injury was measured. The Basso-Beattie-Bresnahan scoring method, inclined plate grasp experiments, and footprint experiments were used to evaluate motor function. Neuritin and TNF-α levels in the spinal cord were detected via ELISA, and caspase-3 level was detected via Western blotting. Spinal cord morphology was assessed via hematoxylin-eosin staining. Apoptosis in the spinal cord was assessed using Fluoro-Jade B staining. Recovery times for spontaneous urination and motor function were shorter in the spinal cord injury + asiaticoside group than in the spinal cord injury group. The neuritin level was increased and TNF-α and caspase-3 levels were decreased in the spinal cord injury + asiaticoside group. Morphological integrity of neurons was better in the spinal cord injury + asiaticoside group than in the spinal cord injury group. It can thus be said that asiaticoside decreased apoptosis of spinal cord neurons. Asiaticoside exerts protective effects against spinal cord injury in rats,…
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Asiaticoside treatment significantly reduces expression of the pro-inflammatory marker TNF-α in spinal cord tissue and serum of SCI-induced rats, blocks apoptosis of spinal cord neurons and enhances neuronal survival, promoting recovery.
“Asiaticoside treatment has proven to significantly reduce the expression of the pro‐inflammatory marker TNF‐α in spinal cord tissue, as revealed by low TNF‐α levels in the serum of SCI‐induced rats in a reported study. Moreover, the triterpene functioned by blocking the apoptosis of spinal cord neurons and enhancing neuronal survival, thereby promoting recovery. 70”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [70]
Cites
Repertoire of microglial and macrophage responses after spinal cord injury
David, S., & Kroner, A. (2011) · Nature reviews. Neuroscience · doi:10.1038/nrn3053 · Cited by 1,437 · see citing works
Harkema, S., Gerasimenko, Y., Hodes, J., Burdick, J., Angeli, C., Chen, Y., Ferreira, C., Willhite, A., Rejc, E., Grossman, R. G., et al. (2011) · The Lancet · doi:10.1016/s0140-6736(11)60547-3 · Cited by 1,229 · see citing works
Neuronal and Glial Apoptosis after Traumatic Spinal Cord Injury
Liu, X., Xu, X., Hu, R., Du, C., Zhang, S. X., Mcdonald, J. W., Dong, H.-X., Wu, Y. J., Fan, G., Jacquin, M., et al. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-14-05395.1997 · Cited by 943 · see citing works
Decoding the organization of spinal circuits that control locomotion
Kiehn, O. (2016) · Nature Reviews Neuroscience · doi:10.1038/nrn.2016.9 · Cited by 742 · see citing works
A brain–spine interface alleviating gait deficits after spinal cord injury in primates
Capogrosso, M., Milekovic, T., Borton, D., Wagner, F., Moraud, E. M., Mignardot, J.-B., Buse, N., Gandar, J., Barraud, Q., Xing, D., et al. (2016) · Nature · doi:10.1038/nature20118 · Cited by 635 · see citing works
Review of Current Evidence for Apoptosis After Spinal Cord Injury
Beattie, M. S., Farooqui, A. A., & Bresnahan, J. C. (2000) · Journal of Neurotrauma · doi:10.1089/neu.2000.17.915 · Cited by 523 · see citing works
Kunkel‐Bagden, E., Dai, H., & Bregman, B. S. (1993) · Experimental Neurology · doi:10.1006/exnr.1993.1017 · Cited by 295 · see citing works
Kwon, B. K., Okon, E. B., Hillyer, J. E., Mann, C., Baptiste, D. C., Weaver, L. C., Fehlings, M. G., & Tetzlaff, W. (2010) · Journal of Neurotrauma · doi:10.1089/neu.2009.1149 · Cited by 280 · see citing works
Papa, S., Rossi, F., Ferrari, R., Mariani, A., Paola, M. D., Caron, I., Fiordaliso, F., Bisighini, C., Sammali, E., Colombo, C., et al. (2013) · ACS Nano · doi:10.1021/nn4036014 · Cited by 160 · see citing works
Potential Role of Natural Compounds Against Skin Aging
Tundis, R., Loizzo, M. R., Bonesi, M., & Menichini, F. (2015) · Current Medicinal Chemistry · doi:10.2174/0929867322666150227151809 · Cited by 119 · see citing works
Predictors of Early Mortality After Traumatic Spinal Cord Injury
Varma, A. K., Hill, E. G., Nicholas, J. S., & Selassie, A. W. (2010) · Spine · doi:10.1097/brs.0b013e3181ba1359 · Cited by 104 · see citing works
Current Concepts in the Management of Acute Spinal Cord Injury
Olby, N. J. (1999) · Journal of Veterinary Internal Medicine · doi:10.1111/j.1939-1676.1999.tb01453.x · Cited by 101 · see citing works
Zhang, L., Li, H.-Z., Gong, X., Luo, F.-L., Wang, B., Hu, N., Wang, C.-D., Zhang, Z., & Wan, J.-Y. (2010) · Phytomedicine · doi:10.1016/j.phymed.2010.01.008 · Cited by 79 · see citing works
LUO, Y., FU, C., WANG, Z., ZHANG, Z., WANG, H., & LIU, Y. (2015) · Molecular Medicine Reports · doi:10.3892/mmr.2015.4425 · Cited by 57 · see citing works
Diaphragm electromyographic activity following unilateral midcervical contusion injury in rats
Rana, S. D., Sieck, G. C., & Mantilla, C. B. (2016) · Journal of Neurophysiology · doi:10.1152/jn.00727.2016 · Cited by 53 · see citing works
Exogenous Neuritin Promotes Nerve Regeneration After Acute Spinal Cord Injury in Rats
Gao, R., Li, X., Xi, S., Wang, H., Zhang, H., Zhu, J., Shan, L., Song, X., Luo, X., Yang, L., et al. (2016) · Human Gene Therapy · doi:10.1089/hum.2015.159 · Cited by 50 · see citing works
Self-citationNeuritin, A Neurotrophic Factor in Nervous System Physiology
Zhou, S., & Zhou, J. (2014) · Current Medicinal Chemistry · doi:10.2174/0929867321666131218093327 · Cited by 44 · see citing works
Nerve Protective Effect of Asiaticoside against Ischemia-Hypoxia in Cultured Rat Cortex Neurons
Sun, T., Liu, B., & Li, P. (2015) · Medical Science Monitor · doi:10.12659/msm.894024 · Cited by 42 · see citing works
Zhang, Y., Jin, H., Wang, J., Wen, L.-Y., Yang, Y., Ruan, J., Zhang, S., Ling, E., Ding, Y., & Zeng, Y. (2017) · Neural Plasticity · doi:10.1155/2017/7351238 · Cited by 40 · see citing works
Chen, Y., Wang, B., & Zhao, H. P. (2018) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2018.6072 · Cited by 36 · see citing works
Zhang, Z., Li, X., Li, D., Luo, M., Li, Y., Li, S., & Jiang, X. (2016) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2016.4004 · Cited by 35 · see citing works
Self-citation[Efficacy of traditional herbal medicine, Yokukansan on patients with neuropathic pain].
Nakamura, Y., Tajima, K., Kawagoe, I., Kanai, M., & Mitsuhata, H. (2009) · PubMed · Cited by 34 · see citing works
Kang, S., Liu, S., Li, H., Wang, D., & Qi, X. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.27136 · Cited by 27 · see citing works
Jiang, S.-W., Lin, Y., & Hsieh, C. (2018) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2018/8041820 · Cited by 24 · see citing works
Neuritin attenuates early brain injury in rats after experimental subarachnoid hemorrhage
Zhang, H., He, X., Wang, Y., Sun, X., Zhu, L., Lei, C., Yin, J., Li, X., Hou, F., He, W., et al. (2017) · International Journal of Neuroscience · doi:10.1080/00207454.2017.1337013 · Cited by 23 · see citing works
Self-citationHuang, G., Guo, Y., Ma, Y., Pan, Y., Zheng, S., & Wang, J. (2017) · Neural Regeneration Research · doi:10.4103/1673-5374.215264 · Cited by 19 · see citing works
Wang, J., Ma, C., Guo, C., Yuan, R., & Zhan, X. (2016) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2016.05.044 · Cited by 15 · see citing works
Pharmacotherapeutic review and update of idiopathic nephrotic syndrome in children
Manrique‐Rodríguez, S., Fernandez-Llamazares, C. M., & Sanjurjo‐Sáez, M. (2010) · Pharmacy World & Science · doi:10.1007/s11096-010-9380-2 · Cited by 13 · see citing works
Truflandier, K., Beaumont, É., Charbonney, E., Maghni, K., de Marchie, M., & Spahija, J. (2018) · Neuroscience Letters · doi:10.1016/j.neulet.2018.01.028 · Cited by 11 · see citing works
Combination treatment with asiaticoside and rapamycin: A new hope for in-stent restenosis
GUO, T., FANG, M., Zhang, D., & Li, X. (2013) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2013.1155 · Cited by 6 · see citing works
Self-citationTNF-α production in oligodendrocytes after moderate cervical spinal cord injury
Schaal, S. M., & Pearse, D. D. (2006) · Experimental Neurology · doi:10.1016/j.expneurol.2006.02.088 · Cited by 2 · see citing works
Protective Effect and Mechanism of Asiaticoside Against Spinal Cord Injury in Rats
Zhao, G. (2011) · Traditional Chinese Drug Research and Clinical Pharmacology · Cited by 1 · see citing works
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Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review
Bandopadhyay, S., Mandal, S., Ghorai, M., Jha, N. K., Kumar, M., Radha, Ghosh, A., Proćków, J., Pérez de la Lastra, J. M., & Dey, A. (2023) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.17635 · Cited by 220 · see citing works
He, Z., Hu, Y., Niu, Z., Zhong, K., Liu, T., Yang, M., Ji, L., & Hu, W. (2023) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2022.115865 · Cited by 93 · see citing works
Self-citationJafari, A., Khalatbary, A. R., Taghiloo, S., Mirzaie, M. S., Nazar, E., Poorhassan, M., Akbari, E., Asadzadeh, M., Raoofi, A., & Nasiry, D. (2023) · Regenerative Therapy · doi:10.1016/j.reth.2023.09.003 · Cited by 32 · see citing works
A review of neuroprotective properties of Centella asiatica (L.) Urb. and its therapeutic effects
Jiang, J., Han, R., Ren, H., Yao, Y., & Jiang, W. (2025) · Annals of Medicine · doi:10.1080/07853890.2025.2559122 · Cited by 26 · see citing works
Ji, Z.-S., Lin, H.-S., Fu, C.-H., Luo, J.-X., Yang, H., Zhang, G., Wu, W., & Lin, H.-S. (2021) · Neural Regeneration Research · doi:10.4103/1673-5374.313052 · Cited by 22 · see citing works
Zhang, Y., Meng, X., & Liu, K. (2022) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-021-09929-w · Cited by 18 · see citing works
Zhang, Y., Chen, X., Wang, X., Xu, Y., Li, J., Wu, Y., Wang, Z., Zhang, S., Hu, J., & Qi, Q. (2024) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2024.176541 · Cited by 18 · see citing works
Luo, P., Huang, Q.-S., Chen, S., Wang, Y., & Dou, H. (2022) · International Immunopharmacology · doi:10.1016/j.intimp.2022.108864 · Cited by 17 · see citing works
Neuroprotective prospectives of triterpenoids
Bankar, A. A., Nagulwar, V. P., Kotagale, N. R., & Inamdar, N. (2024) · Exploration of neuroscience · doi:10.37349/en.2024.00047 · Cited by 16 · see citing works
Okselni, T., Septama, A. W., Pamungkas, R. A., Rahmi, E. P., Efdi, M., & Koketsu, M. (2023) · South African Journal of Botany · doi:10.1016/j.sajb.2023.02.019 · Cited by 14 · see citing works
A Review of the Potential Use of Antioxidants in Spinal Cord Injuries
Nowacka, A., Śniegocki, M., & Ziółkowska, E. (2025) · Antioxidants · doi:10.3390/antiox14091081 · Cited by 14 · see citing works
Kunjumon, R., Viswanathan, G., Jayasree, D. V., Biju, P. G., Prakash, P., Chakrapani, P. S. B., & Baby, S. (2022) · Canadian Journal of Chemistry · doi:10.1139/cjc-2021-0281 · Cited by 12 · see citing works
Xiao, X., & Zhang, Q. (2022) · Journal of Natural Medicines · doi:10.1007/s11418-022-01653-y · Cited by 7 · see citing works
miR-6315 silencing protects against spinal cord injury through the Smo and anti-ferroptosis pathway
Ma, Z., Fan, Y., Peng, Y., Bian, L., Zhou, J., Wang, L., Xia, Y., Zheng, S., Ji, Y., Han, Y., et al. (2023) · Bioscience Reports · doi:10.1042/bsr20230030 · Cited by 7 · see citing works
Luo, J., Xie, M., Peng, C. Y., Ma, Y., Wang, K., Lin, G., Yang, H., Chen, T., Liu, Q., Zhang, G., et al. (2022) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2022.950586 · Cited by 6 · see citing works
Xu, H., Dong, J., Li, Y., Zhang, L., Yin, J., Zhu, C., Wang, X., Ren, K., Zhang, H., & Zhao, D. (2023) · Journal of Stroke and Cerebrovascular Diseases · doi:10.1016/j.jstrokecerebrovasdis.2023.107391 · Cited by 5 · see citing works
Güneş, M., Kaftan, G., Kılıçlı, B., Öztürk, A. M., Armağan, G., & Karavana, S. Y. (2025) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2025.114938 · Cited by 4 · see citing works
Xu, J., Ren, Z., Niu, T., & Li, S. (2024) · Orthopaedic Surgery · doi:10.1111/os.14002 · Cited by 4 · see citing works
Therapeutic potential of Asiaticoside in wound healing after endoscopic submucosal dissection (ESD)
Li, Q., Chen, W., Xie, Y., & Zhu, H. (2026) · Pharmaceutical Biology · doi:10.1080/13880209.2026.2638602 · Cited by 3 · see citing works
Fan, Y., Chen, R. E., Tang, L., Fu, P., Zhou, J., & Chen, X. (2025) · Journal of Separation Science · doi:10.1002/jssc.70128 · Cited by 2 · see citing works
- Authors
- Fan, L., Li, X., & Liu, T.
- Affiliations
- Zhengzhou University (CN)
- Corresponding author
- Liu, Tao
- Venue
- Journal of Molecular Neuroscience
- Published
- 2020-06-11
- Volume
- 70
- Issue
- 12
- Pages
- 1988-1996
- ISSN
- 0895-8696 (ISSN-L)
- 1559-1166
- OpenAlex
- W3034714285
- Cited by
- 20
- References
- 32
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 5 · 2022: 6 · 2021: 1
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Spinal Cord Injury Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- asiaticoside, spinal cord injury, neuronal apoptosis, functional recovery, neuritin, TNF-α, caspase-3, anti-inflammatory activity, neuroprotection, motor function, bladder function
- MeSH terms
- Animals, Male, Motor Activity, Neurons, Neuropeptides, Spinal Cord, Spinal Cord Injuries, Triterpenes, Tumor Necrosis Factor-alpha, Rats, Sprague-Dawley, Apoptosis, Neuroprotective Agents, Rats, Caspase 3, GPI-Linked Proteins
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