The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Bile Salt Hydrolase (Bsh) Activity Screening of Lactobacilli: In Vitro Selection of Indigenous Lactobacillus Strains with Potential Bile Salt Hydrolysing and Cholesterol-Lowering Ability
Abstract
The bile salt hydrolase (Bsh) activity of probiotic bacterium residing in gastrointestinal tract has often being associated with its cholesterol-lowering effects. Hence, Bsh activity was explored in this study as the criterion for the selection of most potential Bsh-active and cholesterol-lowering indigenous Lactobacillus strains. Forty lactobacilli were adjudged Bsh active after a preliminary screening of 102 lactobacilli and occurrence of Bsh activity correlated well with their natural habitats. Of the 40 shortlisted lactobacilli, fifteen putative Lactobacillus strains were selected and further tested for their comparative Bsh activity. In the end, indigenous Lactobacillus plantarum strains Lp91 and Lp21 were emerged as the promising Bsh-active lactobacilli with their substrate preference inclined more towards glycocholate than other bile acid amino conjugates. In addition, strains Lp91 and Lp21 also exhibited significantly high bile salt deconjugation, cholesterol assimilation and cholesterol co-precipitation ability in vitro. In conclusion, indigenous L. plantarum strains Lp91 and Lp21 may be the promising candidate probiotics to elucidate the ecological significance of probiotic Bsh activity in vivo.
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“Some bacteria of the genus Lactobacillus, such as Lactobacillus plantarum, possess BSH enzymatic activity (Kumar et al., 2012).”
From Akkermansia muciniphila alleviates high‐fat‐diet ‐related metabolic‐associated fatty liver disease by modulating gut microbiota and bile acids (2023) · cites this paper as Kumar et al., 2012
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Bile acid metabolism and signaling, the microbiota, and metabolic disease
Cai, J., Rimal, B., Jiang, C., Chiang, J. Y. L., & Patterson, A. D. (2022) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2022.108238 · Cited by 529
The Role of Gut Microbiota on Cholesterol Metabolism in Atherosclerosis
Vourakis, M., Mayer, G., & Rousseau, G. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22158074 · Cited by 233
Foley, M. H., O’Flaherty, S., Allen, G., Rivera, A. J., Stewart, A. K., Barrangou, R., & Theriot, C. M. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2017709118 · Cited by 205
Wu, Y., Zhang, Q., Ren, Y., & Ruan, Z. (2017) · PLoS ONE · doi:10.1371/journal.pone.0178868 · Cited by 179
Tsai, C., Lin, P.-P., Hsieh, Y.-M., Zhang, Z., Wu, H.-C., & Huang, C.-C. (2014) · The Scientific World JOURNAL · doi:10.1155/2014/690752 · Cited by 149
Metadata
- Authors
- Kumar, R., Grover, S., & Batish, V. K.
- Affiliations
- National Dairy Research Institute (IN)
- Corresponding author
- Kumar, Rajesh
- Batish, Virender Kumar
- Venue
- Probiotics and Antimicrobial Proteins
- Published
- 2012-04-21
- Volume
- 4
- Issue
- 3
- Pages
- 162-172
- ISSN
- 1867-1306 (ISSN-L)
- 1867-1314
- OpenAlex
- W2081706251
- Cited by
- 123
- References
- 41
- Citations by year
- 2026: 16 · 2025: 17 · 2024: 15 · 2023: 9 · 2022: 7 · 2021: 9 · 2020: 8 · 2019: 7 · 2018: 6 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 5 · 2013: 9 · 2012: 1
- FWCI
- 9.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Probiotics and Antimicrobial Proteins (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Keywords
- bile salt hydrolase, Lactobacillus plantarum, cholesterol-lowering effect, bile acid deconjugation, glycocholate, cholesterol assimilation, probiotic strain selection, indigenous lactic acid bacteria
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