Cited by 205Gut microbiota and healthProbiotics and Fermented FoodsDrug Transport and Resistance Mechanisms

Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization

Open access
Foley, Matthew H., O’Flaherty, Sarah, Allen, Garrison, Rivera, Alissa J., Stewart, Allison K., Barrangou, Rodolphe, Theriot, Casey M.·2021·Proceedings of the National Academy of Sciences

Abstract

Significance: The transformation of bile acids (BAs) by the gut microbiota is increasingly recognized as an important factor shaping host health. The prerequisite step of BA metabolism is carried out by bile salt hydrolases (BSHs), which are encoded by select gut and probiotic bacteria. Despite their prevalence, the utility of harboring a bsh is unclear. Here, we investigate the role of BSHs encoded by Lactobacillus acidophilus and Lactobacillus gasseri . We show that BA type and BSH substrate preferences affect in vitro and in vivo growth of both species. These findings contribute to a mechanistic understanding of bacterial survival in various BA-rich niches and inform future efforts to leverage BSHs as a therapeutic tool for manipulating the gut microbiota.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.2017709118

Cites

90 of 90 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [84]D. Hanahan, “ Techniques for transformation of E. coli” in DNA Cloning: A Practical Approach, D. M. Glover, Ed., (IRL Press Ltd., Oxford, UK, 1985), Vol. 1, pp. 109– 135.

Cited by

Sorted by citations

Authors
Foley, M. H., O’Flaherty, S., Allen, G., Rivera, A. J., Stewart, A. K., Barrangou, R., & Theriot, C. M.
Affiliations
North Carolina State University (US)
Corresponding author
  • Barrangou, Rodolphe
  • Theriot, Casey M.
Venue
Proceedings of the National Academy of Sciences
Published
2021-02
Volume
118
Issue
6
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W3128625686
Cited by
205
References
90
Citations by year
2026: 49 · 2025: 38 · 2024: 29 · 2023: 40 · 2022: 32 · 2021: 17
FWCI
9.26
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Office of Extramural Research, National Institutes of Health (T32DK07737, P30DK034987)
  • DuPont (BSH)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
bile salt hydrolase, Lactobacillus acidophilus, Lactobacillus gasseri, bile acid metabolism, substrate specificity, bacterial fitness, host colonization, gut microbiota, probiotics
MeSH terms
Gastrointestinal Microbiome, Amidohydrolases, Humans, Lactobacillus, Substrate Specificity, Ecosystem, Probiotics, Host-Pathogen Interactions, Genetic Fitness

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review