Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions
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Activity of acetyl-CoA carboxylase (ACCase) and lipid accumulation were assayed in the microalga Chlorella vulgaris co-immobilised in alginate beads with Azospirillum brasilense, under autotrophic and heterotrophic growth conditions, with and without ammonium starvation. ACCase is a key enzyme in de novo fatty acid biosynthesis. Under the two growth conditions, co-immobilisation always enhanced the activity of ACCase and yielded a higher level of lipids when compared with immobilisation of the alga alone. The highest lipid content obtained under autotrophic conditions was with ammonium starvation. Cultivation under heterotrophic conditions without limitation of nitrogen, with or without the presence of bacteria, yielded a higher growth rate and accumulated more lipids than under autotrophic conditions. No correlation was found between total lipids and ACCase activity. Unusually, ammonium starvation significantly reduced lipid accumulation under heterotrophic conditions. Consequently, co-immobilisation, sufficient ammonium and heterotrophic growth conditions were the most significant parameters for lipid accumulation and ACCase activity in C. vulgaris where the two latter parameters are not directly linked.
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A. brasilense affects activities of key enzymes in metabolic pathways in Chlorella spp., resulting in enhancement of production of metabolites of biotechnological importance.
“Several studies have shown that A. brasilense affects the activities of key enzymes in metabolic pathways in Chlorella spp., resulting in the enhancement in production of metabolites of biotechnological importance, as is discussed later.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [66]
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Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia
González-González, L. M., & de-Bashan, L. E. (2021) · Biology · doi:10.3390/biology10040282 · Cited by 133 · see citing works
Self-citation
- Authors
- Leyva, L. A., Bashan, Y., & de-Bashan, L. E.
- Affiliations
- Centro de Investigaciones Biológicas del Noroeste S.C. (MX)
- Venue
- Annals of Microbiology
- Published
- 2014-04-09
- Volume
- 65
- Issue
- 1
- Pages
- 339-349
- ISSN
- 0003-4649 (ISSN-L)
- 1590-4261
- 1869-2044
- OpenAlex
- W2096169304
- Cited by
- 34
- References
- 66
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 3 · 2021: 4 · 2020: 5 · 2019: 1 · 2017: 3 · 2016: 3 · 2015: 7 · 2014: 1
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Annals of Microbiology (publishedVersion)
- Funders
- Consejo Nacional de Ciencia y Tecnología (130656)
- Centro de Investigaciones Biológicas del Noroeste
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- acetyl-CoA carboxylase, lipid accumulation, Chlorella vulgaris, Azospirillum brasilense, alginate beads, heterotrophic growth, autotrophic growth, fatty acid synthesis, microalgae-bacteria co-culture, nitrogen limitation
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