Cited by 73Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionEnzyme Catalysis and Immobilization

Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: I. Autotrophic conditions

Choix, Francisco J., de-Bashan, Luz E., Bashan, Yoav·2012·Enzyme and Microbial Technology

Abstract

The effect of the microalgae-growth promoting bacterium Azospirillum brasilense on accumulation of total carbohydrates and starch in two species of Chlorella (Chlorella vulgaris and Chlorella sorokiniana), when the bacterium and each microalga were jointly immobilized in alginate beads was studied under autotrophic conditions for 144 h in synthetic medium. The interaction of the bacterium with the microalgae enhanced accumulation of total carbohydrate and starch. Cells of Chlorella accumulated the highest amounts of carbohydrate after incubation for 24h. Yet, this did not coincide with the highest affinity and volumetric productivity measured in these cultures. However, after incubation for 72 h, mainly in jointly immobilized treatments of both microalgae species, the cultures reached their highest total carbohydrate content (mainly as starch) and also the highest affinity and volumetric productivity. These results demonstrate the potential of A. brasilense to affect carbohydrates and starch accumulation in Chlorella spp. when both microorganisms are co-cultured, which can be an important tool for applications of microalgae.

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Crossref · 10.1016/j.enzmictec.2012.07.013

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  • In these studies, the authors reported up to 72% and 90% increments, respectively, in total carbohydrates of C. vulgaris under autotrophic and heterotrophic conditions.

    “In these studies, the authors reported up to 72% and 90% increments, respectively, in total carbohydrates of C. vulgaris under autotrophic and heterotrophic conditions”
  • C. sorokiniana had an increase in carbohydrate production of around 55% under autotrophic conditions and 21% under heterotrophic conditions.

    “while C. sorokiniana had an increase in carbohydrate production of around 55% under autotrophic conditions and 21% under heterotrophic conditions.”
  • Co-cultivation of two Chlorella strains (C. vulgaris and C. sorokiniana) with A. brasilense supports the positive effect of the bacteria on carbohydrate production of this microalgae genus.

    “The co-cultivation of two Chlorella strains (C. vulgaris and C. sorokiniana) with A. brasilense supports the positive effect of the bacteria on carbohydrate production of this microalgae genus [70,79].”

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Authors
Choix, F. J., de-Bashan, L. E., & Bashan, Y.
Affiliations
Centro de Investigaciones Biológicas del Noroeste S.C. (MX)
Venue
Enzyme and Microbial Technology
Publisher
Elsevier BV
Published
2012-10
Volume
51
Issue
5
Pages
294-299
ISSN
0141-0229 (ISSN-L)
OpenAlex
W4254755968
Cited by
72
References
43
Citations by year
2026: 1 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 9 · 2021: 8 · 2020: 10 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 6 · 2015: 6 · 2014: 6
FWCI
1.47
Type
journal article
Language
en
Open access
closed
Locations
  • Enzyme and Microbial Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Università degli Studi di Firenze
  • Centro de Investigaciones Biológicas del Noroeste
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
Azospirillum brasilense, Chlorella vulgaris, Chlorella sorokiniana, alginate immobilization, starch accumulation, carbohydrate accumulation, autotrophic cultivation, microalgae-bacteria co-culture, volumetric productivity, biofertilizers
MeSH terms
Alginates, Biotechnology, Chlorella, Culture Media, Hexuronic Acids, Starch, Azospirillum brasilense, Cells, Immobilized, Coculture Techniques, Glucuronic Acid, Chlorella vulgaris, Carbohydrate Metabolism, Autotrophic Processes

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