Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: I. Autotrophic conditions
Abstract
The effect of the microalgae-growth promoting bacterium Azospirillum brasilense on accumulation of total carbohydrates and starch in two species of Chlorella (Chlorella vulgaris and Chlorella sorokiniana), when the bacterium and each microalga were jointly immobilized in alginate beads was studied under autotrophic conditions for 144 h in synthetic medium. The interaction of the bacterium with the microalgae enhanced accumulation of total carbohydrate and starch. Cells of Chlorella accumulated the highest amounts of carbohydrate after incubation for 24h. Yet, this did not coincide with the highest affinity and volumetric productivity measured in these cultures. However, after incubation for 72 h, mainly in jointly immobilized treatments of both microalgae species, the cultures reached their highest total carbohydrate content (mainly as starch) and also the highest affinity and volumetric productivity. These results demonstrate the potential of A. brasilense to affect carbohydrates and starch accumulation in Chlorella spp. when both microorganisms are co-cultured, which can be an important tool for applications of microalgae.
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In these studies, the authors reported up to 72% and 90% increments, respectively, in total carbohydrates of C. vulgaris under autotrophic and heterotrophic conditions.
“In these studies, the authors reported up to 72% and 90% increments, respectively, in total carbohydrates of C. vulgaris under autotrophic and heterotrophic conditions”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [70]
C. sorokiniana had an increase in carbohydrate production of around 55% under autotrophic conditions and 21% under heterotrophic conditions.
“while C. sorokiniana had an increase in carbohydrate production of around 55% under autotrophic conditions and 21% under heterotrophic conditions.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [70]
Co-cultivation of two Chlorella strains (C. vulgaris and C. sorokiniana) with A. brasilense supports the positive effect of the bacteria on carbohydrate production of this microalgae genus.
“The co-cultivation of two Chlorella strains (C. vulgaris and C. sorokiniana) with A. brasilense supports the positive effect of the bacteria on carbohydrate production of this microalgae genus [70,79].”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [70]
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Amelia, R., Akmal, W. R., & Suyono, E. A. (2023) · Jurnal Ilmiah Perikanan dan Kelautan · doi:10.20473/jipk.v15i2.38596 · Cited by 5 · see citing works
Algae-Bacteria Interactomics Unveils Their Role in Growth and Production of High-Value Biorenewables
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Bai, Y., Mori, K., & Toyama, T. (2025) · Bioresource Technology Reports · doi:10.1016/j.biteb.2025.102242 · Cited by 1 · see citing works
Microalgal Biomass: Introduction and Production Methods
Joy, S. R., & Anju, T. R. (2024) · Springer eBooks · doi:10.1007/978-981-99-6727-8_7 · Cited by 1 · see citing works
Bioinformatics: A New Insight Tool to Deal with Environment Management
Tomar, J. (2020) · Springer eBooks · doi:10.1007/978-981-15-7493-1_8
Su, M., Dell’Orto, M., Scaglia, B., D’Imporzano, G., Bani, A., & Adani, F. (2021) · SSRN Electronic Journal · doi:10.2139/ssrn.3953106
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Shang, F. F., Carney, L. T., Weiss, E. L., Abelin, P., Vernet, M., Mendola, D., Mendoza, W. G., & Mitchell, B. G. (2024) · Springer eBooks · doi:10.1007/978-3-031-61253-4_5
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Interaction of Azospirillum with Microalgae
de‐Bashan, L. E., Palacios, O. A., Hernández, J. P., & González-González, L. M. (2025) · Springer eBooks · doi:10.1007/978-3-032-09222-9_15
Self-citationParvez, S. M., & Solaiman, Z. (2024) · Microbial Bioactives · doi:10.25163/microbbioacts.7110662
- Authors
- Choix, F. J., de-Bashan, L. E., & Bashan, Y.
- Affiliations
- Centro de Investigaciones Biológicas del Noroeste S.C. (MX)
- ORCID
- Choix, Francisco J.
- Venue
- Enzyme and Microbial Technology
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 51
- Issue
- 5
- Pages
- 294-299
- ISSN
- 0141-0229 (ISSN-L)
- OpenAlex
- W4254755968
- Cited by
- 72
- References
- 43
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 9 · 2021: 8 · 2020: 10 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 6 · 2015: 6 · 2014: 6
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Enzyme and Microbial Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Università degli Studi di Firenze
- Centro de Investigaciones Biológicas del Noroeste
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- Azospirillum brasilense, Chlorella vulgaris, Chlorella sorokiniana, alginate immobilization, starch accumulation, carbohydrate accumulation, autotrophic cultivation, microalgae-bacteria co-culture, volumetric productivity, biofertilizers
- MeSH terms
- Alginates, Biotechnology, Chlorella, Culture Media, Hexuronic Acids, Starch, Azospirillum brasilense, Cells, Immobilized, Coculture Techniques, Glucuronic Acid, Chlorella vulgaris, Carbohydrate Metabolism, Autotrophic Processes
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