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4.4 Å Resolution Cryo-EM structure of human mTOR Complex 1

Yang, Huirong, Wang, Jia, Liu, Mengjie, Chen, Xizi, Huang, Min, Tan, Dan, Dong, Meng-Qiu, Wong, Catherine C. L., Wang, Jiawei, Xu, Yanhui, Wang, Hong-Wei·2016·Protein & Cell
DOI 10.1007/s13238-016-0346-6PMC PMC5205667
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Figure 1 Size exclusion chromatogram of the human mTORC1 and kinase activity. (A) The gel-filtration was performed using a Superose 6 column (10/3004 GL, GE Healthcare). The peak fractions were subjected to SDS-PAGE and stained with Coomassie blue. (B and C) Phosphorylation of purified S6K1 (K100R) (B) and 4EBP1 (C) by mTORC1 in the presence or absence of Torin. The phosphorylation was detected by immunoblotting with antibodies targeting phospho-Thr-389 (top), Flag (middle), and mTOR (bottom) in (B), and antibodies targeting phospho-4EBP1 (top), 4EBP1 (middle), and mTOR (bottom) in (C). Below are the quantification of the immunoblots for B and C
Figure 1 Size exclusion chromatogram of the human mTORC1 and kinase activity. (A) The gel-filtration was performed using a Superose 6 column (10/3004 GL, GE Healthcare). The peak fractions were subjected to SDS-PAGE and stained with Coomassie blue. (B and C) Phosphorylation of purified S6K1 (K100R) (B) and 4EBP1 (C) by mTORC1 in the presence or absence of Torin. The phosphorylation was detected by immunoblotting with antibodies targeting phospho-Thr-389 (top), Flag (middle), and mTOR (bottom) in (B), and antibodies targeting phospho-4EBP1 (top), 4EBP1 (middle), and mTOR (bottom) in (C). Below are the quantification of the immunoblots for B and CCC BY · PubMed Central
Figure 2 Overall structure of human mTORC1. (A) Colored coded domain architecture of the three essential components of human mTORC1. The same color scheme is used in all structure figures. The inter- and intramolecular interactions are indicated as arrows. B) Ribbon representation of mTORC1 structure in four different views. The proteins and domains are indicated. CaspRaptor represents Caspase-like domain of Raptor
Figure 2 Overall structure of human mTORC1. (A) Colored coded domain architecture of the three essential components of human mTORC1. The same color scheme is used in all structure figures. The inter- and intramolecular interactions are indicated as arrows. B) Ribbon representation of mTORC1 structure in four different views. The proteins and domains are indicated. CaspRaptor represents Caspase-like domain of RaptorCC BY · PubMed Central
Figure 3 The cryo-EM electron potential maps for the interfaces between M-HEAT and Core and between N-HEAT and M-HEAT (D) contoured at 5 sigma level. (A–C) is the different zoom-in view. The putative linkage between N-HEAT and M-HEAT is depicted as the red dot line, which is measured to be 20.4 Å. Because of the high quality of the cryo-EM map, it is clear that the density making up the M-HEAT region does not directly join with the FAT domain. (E and F) Comparison of cryo-EM map from this study at 4.4 Å (E) and previous study at 5.9 Å (F) resolution, respectively. Both of the maps are displayed in Chimera at the same threshold after normalization
Figure 3 The cryo-EM electron potential maps for the interfaces between M-HEAT and Core and between N-HEAT and M-HEAT (D) contoured at 5 sigma level. (A–C) is the different zoom-in view. The putative linkage between N-HEAT and M-HEAT is depicted as the red dot line, which is measured to be 20.4 Å. Because of the high quality of the cryo-EM map, it is clear that the density making up the M-HEAT region does not directly join with the FAT domain. (E and F) Comparison of cryo-EM map from this study at 4.4 Å (E) and previous study at 5.9 Å (F) resolution, respectively. Both of the maps are displayed in Chimera at the same threshold after normalizationCC BY · PubMed Central
Figure 4 Intramolecular and intermolecular interactions within mTORC1. (A) Closed-up view of the dimer formation of mTORC1. Ribbon representation of the mTORC1 is shown. The mTORC1 dimer is formed through the intermolecular interaction between the M-HEAT and the Core and that between the N-HEAT and the Core. (B) Closed-up view of the interaction between Raptor and mTOR. Raptor binds to the ridge region of the M-HEAT repeats in one mTOR and the convex side of the N-HEAT region in the other mTOR
Figure 4 Intramolecular and intermolecular interactions within mTORC1. (A) Closed-up view of the dimer formation of mTORC1. Ribbon representation of the mTORC1 is shown. The mTORC1 dimer is formed through the intermolecular interaction between the M-HEAT and the Core and that between the N-HEAT and the Core. (B) Closed-up view of the interaction between Raptor and mTOR. Raptor binds to the ridge region of the M-HEAT repeats in one mTOR and the convex side of the N-HEAT region in the other mTORCC BY · PubMed Central
Figure 5 Caspase-like domain of Raptor and FKBP12-mTORC1 interaction. (A) Superimposition of caspase-3 and Raptor in mTORC1 structures. Two structures are shown in ribbon representations with unnecessary regions omitted. Caspase-3 is colored in orange. The active site of kinase domain of mTOR and the active site of caspase-like domain of Raptor are indicated, respectively. Shown below is the closed-up view of the structural comparison between caspase-3 and caspase-like domain of Raptor. (B) Equal amount of purified caspase-3 and Raptor were incubated with 20 µmol/L Ac-DEVD-AMC in the presence or absence of caspase inhibitor Z-VAD-FMK at 37°C for 60 min. The activities were measured using a Spectrafluor Fluorescence Plate Reader with excitation at 400 nm and emission at 505 nm. Error bars, s.d. for triplicate experiments. (C) Superimposition of FRBmTOR-FKBP and mTORC1 structures is shown in ribbon representations. (D) Effect of FKBP12-Rapamycin for mTORC1 assembly. Increased amount of FKBP12-Rapamycin incubated with mTORC1 (Flag-Raptor) immobilized on the Flag resin. Bound proteins were subjected to SDS-PAGE and stained with Coomassie blue
Figure 5 Caspase-like domain of Raptor and FKBP12-mTORC1 interaction. (A) Superimposition of caspase-3 and Raptor in mTORC1 structures. Two structures are shown in ribbon representations with unnecessary regions omitted. Caspase-3 is colored in orange. The active site of kinase domain of mTOR and the active site of caspase-like domain of Raptor are indicated, respectively. Shown below is the closed-up view of the structural comparison between caspase-3 and caspase-like domain of Raptor. (B) Equal amount of purified caspase-3 and Raptor were incubated with 20 µmol/L Ac-DEVD-AMC in the presence or absence of caspase inhibitor Z-VAD-FMK at 37°C for 60 min. The activities were measured using a Spectrafluor Fluorescence Plate Reader with excitation at 400 nm and emission at 505 nm. Error bars, s.d. for triplicate experiments. (C) Superimposition of FRBmTOR-FKBP and mTORC1 structures is shown in ribbon representations. (D) Effect of FKBP12-Rapamycin for mTORC1 assembly. Increased amount of FKBP12-Rapamycin incubated with mTORC1 (Flag-Raptor) immobilized on the Flag resin. Bound proteins were subjected to SDS-PAGE and stained with Coomassie blueCC BY · PubMed Central

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Citations

Cites

Research works printed but not linked: 29

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Metadata

Authors
Yang, H., Wang, J., Liu, M., Chen, X., Huang, M., Tan, D., Dong, M.-Q., Wong, C. C. L., Wang, J., Xu, Y., & Wang, H.-W.
Venue
Protein & Cell
Publisher
Oxford University Press (OUP)
Published
2016-12
Volume
7
Issue
12
Pages
878-887
ISSN
  • 1674-800X
  • 1674-8018
DOI
10.1007/s13238-016-0346-6
PMC
PMC5205667
Cited by
96
References
8
Language
en
Licence
creativecommons.org/licenses/by/4.0 (unspecified)

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