Label-free Raman microspectral analysis for comparison of cellular uptake and distribution between nontargeted and EGFR-targeted biodegradable polymeric nanoparticles
Open accessAbstract
Active targeted delivery of nanoparticle-encapsulated agents to tumor cells in vivo is expected to enhance therapeutic effect with significantly less non-specific toxicity. Active targeting is based on surface modification of nanoparticles with ligands that bind with extracellular targets and enhance payload delivery in the cells. In this study, we have used label-free Raman micro-spectral analysis and kinetic modeling to study cellular interactions and intracellular delivery of C6-ceramide using a non-targeted and an epidermal growth factor receptor (EGFR) targeted biodegradable polymeric nano-delivery systems, in EGFR-expressing human ovarian adenocarcinoma (SKOV3) cells. The results show that EGFR peptide-modified nanoparticles were rapidly internalized in SKOV3 cells leading to significant intracellular accumulation as compared to non-specific uptake by the non-targeted nanoparticles. Raman micro-spectral analysis enables visualization and quantification of the carrier system, drug-load, and responses of the biological systems interrogated, without exogenous staining and labeling procedures.
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G11-modified PEG-PLGA nanoparticles are rapidly internalized in ovarian cancer cells compared to nontargeted nanoparticles, leading to significant intracellular accumulation.
“Compared with the cellular uptake of nontargeted nanoparticles, EGFR polypeptide-modified nanoparticles are rapidly internalized in ovarian cancer cells, leading to significant intracellular accumulation.224”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [224]
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- Authors
- Chernenko, T., Buyukozturk, F., Miljkovic, M., Carrier, R., Diem, M., & Amiji, M.
- Affiliations
- Northeastern University (US)
- Corresponding author
- Amiji, Mansoor
- Venue
- Drug Delivery and Translational Research
- Published
- 2013-10-01
- Volume
- 3
- Issue
- 6
- Pages
- 575-586
- ISSN
- 2190-393X (ISSN-L)
- 2190-3948
- PMC
- PMC3843494
- OpenAlex
- W2018686821
- Cited by
- 24
- References
- 36
- Citations by year
- 2025: 4 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 0.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Drug Delivery and Translational Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (R01CA119617, R21CA135594, R01CA090346)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- biodegradable polymer nanoparticles, cellular uptake, intracellular drug distribution, C6-ceramide, active targeting, SKOV3 cells, ovarian adenocarcinoma, kinetic modeling, nanoparticle uptake, tumor targeting
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