Proteolysis of tau by calpain
Abstract
The calpain-induced proteolysis of tau associated with twice-cycled microtubules or from a total brain heat-stable fraction was studied. Twice-cycled microtubule tau was rapidly hydrolyzed by calpain. In contrast, tau purified from the total brain heat-stable fraction was very resistant to degradation by calpain. These results clearly demonstrate that there are at least 2 populations of tau in the brain based on calpain-sensitivity, a calpain-sensitive form that is associated with microtubules and a calpain-resistant form that may represent another population of tau in the brain.
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Calpain-2 cleaves tau at Arg230.
“while calpain-2 cleaves tau at Arg230 [202–206].”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [202]
Calpain-1 cleaves tau at Lys44, Arg230, Arg242, Gly323, and Gly326.
“Calpain-1 cleaves tau at Lys44, Arg230, Arg242, Gly323, and Gly326”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [202]
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Self-citationAnti-tau oligomers passive vaccination for the treatment of Alzheimer disease
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Activation of calpain‐1 in myelin and microglia in the white matter of the aged rhesus monkey
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Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice
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Stem Cells as Potential Targets of Polyphenols in Multiple Sclerosis and Alzheimer’s Disease
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Identification and characterization of PEBP as a calpain substrate
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Proteolysis of Microtubule‐Associated Protein 2 and Tubulin by Cathepsin D
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Self-citationNeighbored phosphorylation sites as PHF-tau specific markers in Alzheimer’s disease
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Brain G-protein proteolysis by calpain: enhancement by lithium
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Self-citationEthanol enhances tau accumulation in neuroblastoma cells that inducibly express tau
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- Authors
- Johnson, G. V. W., Jope, R. S., & Binder, L. I.
- Affiliations
- University of Alabama at Birmingham (US)
- Corresponding author
- Johnson, Gail V.W.
- ORCID
- Johnson, Gail V.W.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1989-09
- Volume
- 163
- Issue
- 3
- Pages
- 1505-1511
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2026101249
- Cited by
- 161
- References
- 18
- Citations by year
- 2025: 2 · 2021: 1 · 2020: 4 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 5 · 2012: 8
- FWCI
- 2.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01ag006569-13)
- National Institute on Aging (P01 AG06569)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Calpain Protease Function and Regulation
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › S100 Proteins and Annexins
- Keywords
- tau protein, calpain, proteolysis, microtubules, tau isoforms
- MeSH terms
- Animals, Brain, Calpain, Cattle, Kinetics, Microtubule-Associated Proteins, Microtubules, Nerve Tissue Proteins, Immunoblotting, tau Proteins
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