The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Rat hippocampal neurons in dispersed cell culture.
Abstract
An in vitro system has been developed for the study of isolated hippocampal neurons from 18- or 19-day rat fetuses. Following trypsinization the cells are plated out at low density on polylysine-treated coverslips in an enriched medium. The isolated neurons rapidly attach to the substrate and initiate process extension. Little reaggregation occurs and the number of non-neuronal cells present is minimal. Unless co-cultured with tissue explants the neurons survive for only a few days; in the presence of hippocampal explants the initial growth of the isolated cells is improved and their survival in culture is extended to about two weeks. Some of the cells in such cultures develop a characteristic branching pattern closely resembling that of maturing hippocampal pyramidal cells in vivo. There is a clear relationship between the stage of the cells' development and their growth in culture. Cells which had completed DNA synthesis about 48 h before dissociation, and which were in the process of migration to the cortical plate, survived best in our cultures. Early post-mitotic cells which were still within the ventricular zone and cells which had already reached the cortical plate grew poorly. This system should permit the study not only of process formation by these cells, but also of their capacity to form specific synapses in vitro and of the biochemical constituents of their surfaces.
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“Hippocampal neurons go through five stages of differentiation (Banker and Cowan, 1977; Dotti et al., 1988).”
From Rassf5 and Ndr kinases act in a novel pathway regulating neuronal polarity through Par3 phosphorylation (2014) · cites this paper as Banker and Cowan, 1977
In the citing paper
“The majority of neurons in the hippocampus at this stage would have undergone their final cell division only 1 or 2 d before this time (Banker and Cowan, 1977;Schlessinger et al., 1978).”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Banker and Cowan, 1977
In the citing paper
“Within a day after plating, most neurons had begun to extend processes, as shown in Figure 4. Typically these included one major process, which at this stage was 50-100 pm in length, and several, much shorter, minor processes (see Banker and Cowan, 1977).”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Banker and Cowan, 1977
Citations
Cites
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Self-citationSchlessinger, A. R., Cowan, W. M., & Gottlieb, D. I. (1975) · The Journal of Comparative Neurology · doi:10.1002/cne.901590202 · Cited by 731
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Surface Movements during the Growth of Single Explanted Neurons
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BRANCHING PATTERNS OF INDIVIDUAL SYMPATHETIC NEURONS IN CULTURE
Bray, D. (1973) · The Journal of Cell Biology · doi:10.1083/jcb.56.3.702 · Cited by 235
Dissociated dorsal root ganglia in tissue culture
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Nerve cell differentiation in vitro
Ludueña, M. A. (1973) · Developmental Biology · doi:10.1016/0012-1606(73)90137-1 · Cited by 132
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Bunge, R. P., & Wood, P. M. (1973) · Brain Research · doi:10.1016/0006-8993(73)90135-2 · Cited by 105
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Self-citationDissociation, fractionation and culture of chick embryo sympathetic ganglionic cells
Varon, S. S., & Raiborn, C. (1972) · Journal of Neurocytology · doi:10.1007/bf01099934 · Cited by 101
Fischbach, G. D., & Dichter, M. A. (1974) · Developmental Biology · doi:10.1016/0012-1606(74)90172-9 · Cited by 84
Bornstein, M. B., & Model, P. G. (1972) · Brain Research · doi:10.1016/0006-8993(72)90673-7 · Cited by 75
The growth of spinal ganglion neurons in serum-free medium
Ludueña, M. A. (1973) · Developmental Biology · doi:10.1016/0012-1606(73)90152-8 · Cited by 62
Bunge, R. P., Rees, R. P., Wood, P. M., Burton, H., & Ko, C. (1974) · Brain Research · doi:10.1016/0006-8993(74)90056-0 · Cited by 58
Varon, S. S., Raiborn, C., & Burnham, P. A. (1974) · PubMed · Cited by 52
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RAPID EMBEDDING OF CELL CULTURE MONOLAYERS AND SUSPENSIONS FOR ELECTRON MICROSCOPY
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Orientational preferences shown by microspikes of growing nerve cells in vitro
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Research works printed but not linked: 1
- Cotman, 1974, The making of a synapse, Vol. 1, 1
Cited by
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Brewer, G. J., Torricelli, J. R., Evege, E. K., & Price, P. J. (1993) · Journal of Neuroscience Research · doi:10.1002/jnr.490350513 · Cited by 2,325
Neurotrophic and Neurotoxic Effects of Amyloid β Protein: Reversal by Tachykinin Neuropeptides
Yankner, B. A., Duffy, L. K., & Kirschner, D. A. (1990) · Science · doi:10.1126/science.2218531 · Cited by 1,975
Kaech, S., & Banker, G. A. (2006) · Nature Protocols · doi:10.1038/nprot.2006.356 · Cited by 1,766
Self-citationNeurodegeneration induced by beta-amyloid peptides in vitro: the role of peptide assembly state
Pike, C., Burdick, D., Walencewicz, A., Glabe, C., & Cotman, C. (1993) · The Journal of Neuroscience · doi:10.1523/jneurosci.13-04-01676.1993 · Cited by 1,417
Synaptic plasticity, memory and the hippocampus: a neural network approach to causality
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Metadata
- Authors
- Banker, G. A., & Cowan, W. M.
- Affiliations
- Washington University in St. Louis (US)
- ORCID
- Banker, Gary A.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1977-05
- Volume
- 126
- Issue
- 3
- Pages
- 397-425
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1987700551
- Cited by
- 1,110
- References
- 28
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 7 · 2023: 10 · 2022: 14 · 2021: 18 · 2020: 23 · 2019: 20 · 2018: 24 · 2017: 16 · 2016: 21 · 2015: 24 · 2014: 34 · 2013: 19 · 2012: 26
- FWCI
- 3.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- hippocampal neurons, neuronal isolation, neuronal survival, cortical plate, ventricular zone, neuronal migration, DNA synthesis, synaptogenesis
- MeSH terms
- Animals, Axons, Cell Aggregation, Cell Division, Cell Movement, Cell Survival, Cells, Cultured, Hippocampus, Neurons, Rats
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