Cited by 42

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A rapid flat gel isoelectric focusing method for the determination of apolipoprotein E phenotypes and its application

Eto, M., Watanabe, K., Ishii, K.·1985·Clinica Chimica Acta

Abstract

A rapid flat gel isoelectric focusing method has been developed for the determination of apolipoprotein E phenotypes. Isoelectric focusing in 5% polyacrylamide flat gel with 8 mol/l urea and 2.8% pharmalyte (pH 4-6.5) was carried out at 3,000 V and 4 degrees C for 1 h under a constant power of 30 W. The separation of apolipoprotein E isoproteins was good and the isoelectric points were determined. Using this method, the apolipoprotein E phenotype frequency was examined in the Japanese population, and a higher frequency of phenotype E3/3 and a lower frequency of phenotype E3/2 were found in Japanese than those reported for the German, American or English population. In our focusing system the cut-off point of apolipoprotein E2/E3 ratio between the phenotype E3/3 and E3/2 was assumed to be approximately 0.9. These results indicate that this method may be useful for the determination of apolipoprotein E phenotypes.

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Research works printed but not linked: 18

  1. Shelburne, 1980, Effect of apoproteins on hepatic uptake of triglyceride emulsions in the rat, J Clin Invest, 65, 652, 10.1172/JCI109710 · DOI
  2. Sherrill, 1980, Rapid hepatic clearance of the canine lipoproteins containing only the E apoprotein by a high affinity receptor, J Biol Chem, 255, 1804, 10.1016/S0021-9258(19)85951-3 · DOI
  3. Innerarity, 1978, Enhanced binding by cultured human fibroblasts of apo-E-containing lipoproteins as compared with low density lipoproteins, Biochemistry, 17, 1440, 10.1021/bi00601a013 · DOI · Paper page
  4. Utermann, 1975, Familial hyperlipoproteinemia type III: Deficiency of a specific apolipoprotein (apo E-III) in the very-low-density lipoproteins, FEBS Lett, 56, 352, 10.1016/0014-5793(75)81125-2 · DOI · Paper page
  5. Utermann, 1977, Polymorphism of apolipoprotein E and occurrence of dysbetali-poproteinemia in man, Nature (London), 269, 604, 10.1038/269604a0 · DOI · Paper page
  6. Pagnan, 1977, Characterization of human very low density lipoproteins containing two electrophoretic populations: double pre-beta lipoproteinemia and primary dysbetali-poproteinemia, J Lipid Res, 18, 613, 10.1016/S0022-2275(20)41603-7 · DOI
  7. Weisgraber, 1982, Abnormal lipoprotein receptor-binding activity of the human E apoprotein due to cysteine-arginine interchange at a single site, J Biol Chem, 257, 2518, 10.1016/S0021-9258(18)34954-8 · DOI · Paper page
  8. Havel, 1982, Familial dysbetalipoproteinemia, new aspects of pathogenesis and diagnosis, Med Clin North Am, 66, 441, 10.1016/S0025-7125(16)31429-8 · DOI
  9. Gumming, 1984, Polymorphism at the apoprotein-E locus in relation to risk of coronary disease, Clin Genet, 25, 310, 10.1111/j.1399-0004.1984.tb01995.x · DOI
  10. Ghiselli, 1982, Increased prevalence of apolipoprotein E4 in type V hyperlipoproteinemia, J Clin Invest, 70, 474, 10.1172/JCI110638 · DOI
  11. Kameda, 1984, Increased frequency of lipoprotein disorders similar to type III hyperlipoproteinemia in survivors of myocardial infarction in Japan, Atherosclerosis, 51, 241, 10.1016/0021-9150(84)90171-0 · DOI
  12. Utermann, 1978, Polymorphism of apolipoprotein E. 1. Methodological aspects and diagnosis of hyperlipoproteinemia type III without ultracentrifugation, Clin Genet, 14, 351, 10.1111/j.1399-0004.1978.tb02101.x · DOI
  13. Warnick, 1979, Gel isoelectric focusing method for specific diagnosis of familial hyperlipoproteinemia type 3, Clin Chem, 25, 279, 10.1093/clinchem/25.2.279 · DOI · Paper page
  14. Douste-Blazy, 1982, Increased frequency of apo E-ND phenotype and hyperapobetalipoproteinemia in normolipidemic subjects with xanthelasmas of the eyelids, Ann Int Med, 96, 164, 10.7326/0003-4819-96-2-164 · DOI
  15. Weidmann, 1979, Type III hyperlipoproteinemia: development of a VLDL apo E gel isoelectric focusing technique and application in family studies, J Lab Clin Med, 93, 549
  16. Havel, 1955, The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum, J Clin Invest, 34, 1345, 10.1172/JCI103182 · DOI
  17. Yamamura, 1984, A new isoform of apolipoprotein E-apo E-5-associated with hyperlipidemia and atherosclerosis, Atherosclerosis, 50, 159, 10.1016/0021-9150(84)90019-4 · DOI · Paper page
  18. Utermann, 1980, Genetics of the apolipoprotein E system in man, Am J Hum Genet, 32, 339

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Metadata

Authors
Eto, M., Watanabe, K., & Ishii, K.
Venue
Clinica Chimica Acta
Publisher
Elsevier BV
Published
1985-06
Volume
149
Issue
1
Pages
21-28
ISSN
0009-8981
Cited by
42
References
2
Language
en

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