Cited by 1,237DNA Repair MechanismsCarcinogens and Genotoxicity AssessmentEffects of Radiation Exposure

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall

Bohr, V·1985·Cell

Open this paper in CoChat — free account

Abstract

DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2), while little removal was detected in fragments upstream of the gene and only 15% were removed from the genome overall. This suggests that damage processing can vary according to function or activity of affected sequences, which has general implications for correlations of DNA repair with survival and mutagenesis. Perhaps preferential repair of vital sequences facilitates UV-resistance of these cells despite low overall repair levels.

Integrity signals

Registry

Crossref · 10.1016/0092-8674(85)90150-3

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Wahl, G. M., Stern, M., & Stark, G. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3683 · Cited by 3,159
Calcium-dependent bacteriophage DNA infection
Mandel, M., & Higa, A. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90051-3 · Cited by 2,521
[29] Analysis of repeating DNA sequences by reassociation
Britten, R. J., Graham, D. E., & Neufeld, B. R. (1974) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(74)29033-5 · Cited by 1,041
Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.
Alt, F. W., Kellems, R. E., Bertino, J. R., & SCHIMKE, R. T. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34875-5 · Cited by 934
DNA Repair in Bacteria and Mammalian Cells
Hanawalt, P. C., Cooper, P. K., Ganesan, A. K., & Smith, C. A. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.004031 · Cited by 837
Gene amplification in cultured animal cells
SCHIMKE, R. T. (1984) · Cell · doi:10.1016/0092-8674(84)90406-9 · Cited by 635
Practical Methods in Molecular Biology
Schleif, R., & Wensink, P. C. (1981) · Springer eBooks · doi:10.1007/978-1-4612-5956-5 · Cited by 473
Sequence organization of the human genome
Schmid, C. W., & Deininger, P. L. (1975) · Cell · doi:10.1016/0092-8674(75)90184-1 · Cited by 301

Show all 34 references

Sorted by citations

Items 201–300 of 1,001

Repair of alkylation and oxidative damage in mitochondrial DNA
LeDoux, S. P., Driggers, W. J., Hollensworth, B. S., & Wilson, G. L. (1999) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(99)00026-9 · Cited by 110
UVB radiation-induced cancer predisposition in Cockayne syndrome group A (Csa) mutant mice
van der Horst, G. T. J., Meira, L. B., Gorgels, T. G. M. F., de Wit, J., Velasco-Miguel, S., Richardson, J. A., Kamp, Y., Vreeswijk, M. P. G., Smit, B., Bootsma, D., et al. (2002) · DNA repair · doi:10.1016/s1568-7864(01)00010-6 · Cited by 110
Preferential repair of DNA damage on the transcribed strand of the human metallothionein genes requires RNA polymerase II
Leadon, S. A., & Lawrence, D. A. (1991) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(91)90019-l · Cited by 108
Role of high mobility group (HMG) chromatin proteins in DNA repair
Reeves, R., & Adair, J. E. (2005) · DNA repair · doi:10.1016/j.dnarep.2005.04.010 · Cited by 107
DNA repair fine structure and its relations to genomic instability
Bohr, V. A. (1995) · Carcinogenesis · doi:10.1093/carcin/16.12.2885 · Cited by 106
Self-citation
Model for XPC-independent Transcription-coupled Repair of Pyrimidine Dimers in Humans
Mu, D., & Sancar, A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7570 · Cited by 107
Transcription-Coupled DNA Repair: From Mechanism to Human Disorder
van den Heuvel, D., van der Weegen, Y., Boer, D. E. C., Ogi, T., & Luijsterburg, M. S. (2021) · Trends in Cell Biology · doi:10.1016/j.tcb.2021.02.007 · Cited by 105
Effect of DNA damage on PCR amplification efficiency with the relative threshold cycle method
Sikorsky, J. A., Primerano, D. A., Fenger, T. W., & Denvir, J. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.08.168 · Cited by 111
“Lesionspecific digestion reduces the signal intensity of the target gene and the frequency of lesions is proportional to the decrease in probe hybridization [9,10].”
Background
Molecular Mechanisms of the Whole DNA Repair System: A Comparison of Bacterial and Eukaryotic Systems
Morita, R., Nakane, S., Shimada, A., Inoue, M., Iino, H., Wakamatsu, T., Fukui, K., Nakagawa, N., Masui, R., & Kuramitsu, S. (2010) · Journal of Nucleic Acids · doi:10.4061/2010/179594 · Cited by 117
“In 1985, it became apparent that the DNA lesion in the transcribed strand is preferentially repaired [118].”
Background
Relationship among DNA double‐strand break (DSB), DSB repair, and transcription prevents genome instability and cancer
Ui, A., Chiba, N., & Yasui, A. (2020) · Cancer Science · doi:10.1111/cas.14404 · Cited by 104
“During transcription: (1) TLP, which has a function in the initiation stage; and (2) ENL and (3) NELF-F, which have a function in the elongation stage, have been reported to be involved in DSBinduced transcriptional repression (Figure 2).”
Background
Inhibition of transcription and strand-specific DNA repair by α-amanitin in Chinese hamster ovary cells
Christians, F. C., & Hanawalt, P. C. (1992) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(92)90056-9 · Cited by 104
Drosophila ribosomal protein S3 contains an activity that cleaves DNA at apurinic/apyrimidinic sites.
Wilson, D. M., Deutsch, W. A., & Kelley, M. R. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47256-0 · Cited by 103
Ionizing radiation and genetic risks III. Nature of spontaneous and radiation-induced mutations in mammalian in vitro systems and mechanisms of induction of mutations by radiation
Sankaranarayanan, K. (1991) · Mutation Research/Reviews in Genetic Toxicology · doi:10.1016/0165-1110(91)90029-u · Cited by 103
Activation of p70 Ribosomal Protein S6 Kinase Is an Essential Step in the DNA Damage-dependent Signaling Pathway Responsible for the Ultraviolet B-mediated Increase in Interstitial Collagenase (MMP-1) and Stromelysin-1 (MMP-3) Protein Levels in Human Dermal Fibroblasts
Brenneisen, P., Wenk, J., Wlaschek, M., Krieg, T., & Scharffetter‐Kochanek, K. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.6.4336 · Cited by 102
Correlation between molecular clock ticking, codon usage, fidelity of DNA repair, chromosome banding and chromatin compactness in germline cells
Filipski, J. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80660-9 · Cited by 104
Stranded in an active gene
Hanawalt, P. C., & Mellon, I. (1993) · Current Biology · doi:10.1016/0960-9822(93)90156-i · Cited by 102
Comet-FISH with strand-specific probes reveals transcription-coupled repair of 8-oxoGuanine in human cells
Guo, J., Hanawalt, P. C., & Spivak, G. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt524 · Cited by 98
Nucleotide Excision Repair in E. Coli and Man
Sancar, A., & Reardon, J. T. (2004) · Advances in protein chemistry · doi:10.1016/s0065-3233(04)69002-4 · Cited by 105
Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus
Machius, M., Henry, L., Palnitkar, M., & Deisenhofer, J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.21.11717 · Cited by 104
Characterization of a DNA repair domain containing the dihydrofolate reductase gene in Chinese hamster ovary cells.
Bohr, V. A., Okumoto, D. S., Ho, L., & Hanawalt, P. C. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66617-7 · Cited by 100
Self-citation
Do mutator mutations fuel tumorigenesis?
Fox, E. J., Prindle, M. J., & Loeb, L. A. (2013) · Cancer and Metastasis Reviews · doi:10.1007/s10555-013-9426-8 · Cited by 109
“Subsequently, it has been shown that individuals with this disease carry defects in genes which encode proteins involved in nucleotide excision repair [27, 28] and that engineered mutations in the murine homologs of these genes result in an increase in cancer incidence upon exposure to DNA-damaging agents (reviewed in…”
Mfd, the bacterial transcription repair coupling factor: translocation, repair and termination
Roberts, J., & Park, J. (2004) · Current Opinion in Microbiology · doi:10.1016/j.mib.2004.02.014 · Cited by 105
Double Mutants of Saccharomyces cerevisiae with Alterations in Global Genome and Transcription-Coupled Repair
Verhage, R. A., Van Gool, A. J., Groot, N. D., Hoeijmakers, J. H. J., Putte, P. V. D., & Brouwer, J. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.2.496 · Cited by 98
What histone code for DNA repair?
Escargueil, A. E., Soares, D. G., Salvador, M. M., Larsen, A. K., & Henriques, J. A. P. (2008) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2008.01.004 · Cited by 101
“Moreover, UV-induced CPDs are repaired more rapidly in transcriptionally active versus inactive regions of the genome, and within transcribed and non-transcribed strands of expressed genes [70,71].”
Localized torsional tension in the DNA of human cells.
Ljungman, M., & Hanawalt, P. (1992) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.89.13.6055 · Cited by 96
Mitochondrial DNA damage triggers mitochondrial dysfunction and apoptosis in oxidant-challenged lung endothelial cells
Ruchko, M. V., Gorodnya, O. M., LeDoux, S. P., ALEXEYEV, M. F., Al‐Mehdi, A., & Gillespie, M. N. (2004) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00255.2004 · Cited by 98
Effects of UV, 4NQO and TPA on gene expression in cultured human epidermal keratinocytes
Kartašova, T., Cornelissen, B. J. C., Belt, P. B. G. M., & van de Putte, P. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.15.5945 · Cited by 98
Enhanced Repair of Cyclobutane Pyrimidine Dimers and Improved UV Resistance in Photolyase Transgenic Mice
Schul, W., Jans, J., Rijksen, Y. M. A., Klemann, K. H. M., Eker, A. P. M., de Wit, J., Nikaido, O., Nakajima, S., Yasui, A., Hoeijmakers, J. H. J., et al. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf456 · Cited by 96
DNA repair in neural cells: basic science and clinical implications
Brooks, P. J. (2002) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/s0027-5107(02)00222-1 · Cited by 98
Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II. Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells
McKelvey‐Martin, V. J., Ho, E. T. S., McKeown, S. R., Johnston, S. R., McCarthy, P. J., Rajab, N. F., & Downes, C. S. (1998) · Mutagenesis · doi:10.1093/mutage/13.1.1 · Cited by 97
Induction of the SOS Response Increases the Efficiency of Global Nucleotide Excision Repair of Cyclobutane Pyrimidine Dimers, but Not 6-4 Photoproducts, in UV-Irradiated Escherichia coli
Crowley, D. J., & Hanawalt, P. C. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.13.3345-3352.1998 · Cited by 96
Strand specificity for UV-induced DNA repair and mutations in the Chinese hamster HPRT gene
Vrieling, H., Venema, J., van Rooyen, M. L., van Hoffen, A., Menichini, P., Zdzienicka, M. Z., Simons, J. W. I. M., Mullenders, L. H. F., & van Zeeland, A. A. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.9.2411 · Cited by 96
The Sensitivity of Cockayne’s Syndrome Cells to DNA-Damaging Agents Is Not due to Defective Transcription-Coupled Repair of Active Genes
van Oosterwijk, M. F., Versteeg, A., Filon, R., van Zeeland, A. A., & Mullenders, L. H. F. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.8.4436 · Cited by 95
“Intensities of bands in autoradiograms were quantified, and lesion frequencies were calculated from the ratio of the band intensities of full-size fragments in lanes containing UvrABC-treated DNA to those of full-size fragments in lanes containing nontreated DNA by employing the Poisson distribution (5).”
Method
Preferential DNA repair of (6‐4) photoproducts in the dihydrofolate reductase gene of Chinese hamster ovary cells
Thomas, D. C., Okumoto, D. S., Sancar, A., & Bohr, V. A. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84671-9 · Cited by 94
Self-citation
Transcription arrest at DNA damage sites
Tornaletti, S. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2005.03.014 · Cited by 93
Roles for the transcription elongation factor NusA in both DNA repair and damage tolerance pathways in Escherichia coli
Cohen, S., Lewis, C. A., Mooney, R. A., Kohanski, M. A., Collins, J. J., Landick, R. C., & Walker, G. C. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1005203107 · Cited by 95
Nucleotide excision repair and its interplay with transcription
van Hoffen, A., Balajee, A. S., van Zeeland, A. A., & Mullenders, L. H. F. (2003) · Toxicology · doi:10.1016/j.tox.2003.06.001 · Cited by 94
The role of nucleotide excision repair in protecting embryonic stem cells from genotoxic effects of UV-induced DNA damage
Sloun, P. P. H. V., Jansen, J. G., Weeda, G., Mullenders, L. H. F., van Zeeland, A. A., Lohman, P. H. M., & Vrieling, H. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.16.3276 · Cited by 93
Up‐Regulation of Base Excision Repair Activity for 8‐Hydroxy‐2'‐Deoxyguanosine in the Mouse Brain After Forebrain Ischemia‐Reperfusion
Lin, L., Cao, S., Yu, L., Cui, J., Hamilton, W. J., & Liu, P. K. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.741098.x · Cited by 92
Selective repair of specific chromatin domains in UV-irradiated cells from xeroderma pigmentosum complementation group C
Kantor, G. J., Barsalou, L., & Hanawalt, P. C. (1990) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(90)90071-c · Cited by 92
Defective Global Genome Repair in XPC Mice Is Associated with Skin Cancer Susceptibility But Not with Sensitivity to UVB Induced Erythema and Edema
Berg, R. J. W., Ruven, H. J. T., Sands, A., de Gruijl, F. R., & Mullenders, L. H. F. (1998) · Journal of Investigative Dermatology · doi:10.1111/j.1523-1747.1998.00173.x · Cited by 92
DNA repair and recovery of RNA synthesis following exposure to ultraviolet light are delayed in long genes
de Andrade-Lima, L. C., Veloso, A., Paulsen, M. T., Menck, C. F. M., & Ljungman, M. E. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv148 · Cited by 88
“It has been shown that blocked RNA polymerases recruit nucleotide excision repair factors in a CSA- and CSBmediated manner allowing for a preferential repair of active genes (7) in a strand-specific manner (8).”
Background
Autophagy in UV Damage Response
Sample, A., & He, Y. (2016) · Photochemistry and Photobiology · doi:10.1111/php.12691 · Cited by 90
The compositional evolution of vertebrate genomes
Bernardi, G. (2000) · Gene · doi:10.1016/s0378-1119(00)00441-8 · Cited by 90
“…only more detailed investigations performed later definitely proved that themore efficient in some regions, for instance in transcribed sequences (Bohr et al., 1985; see also Balajee and Bohr, mouse genome did not exhibit the GC-richest DNA components that were present in the human genome2000…”
Background
The molecular mechanism of transcription-coupled DNA repair
Savery, N. J. (2007) · Trends in Microbiology · doi:10.1016/j.tim.2007.05.005 · Cited by 88
Analysis of repair of cyclobutane pyrimidine dimers and pyrimidine 6-4 pyrimidone photoproducts in transcriptionally active and inactive genes in Chinese hamster cells.
Vreeswijk, M. P. G., van Hoffen, A., Westland, B., Vrieling, H., van Zeeland, A. A., & Mullenders, L. H. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31774-5 · Cited by 88
Lack of transcription-coupled repair in mammalian ribosomal RNA genes
Christians, F. C., & Hanawalt, P. C. (1993) · Biochemistry · doi:10.1021/bi00090a030 · Cited by 89
CPDs and 6-4PPs play different roles in UV-induced cell death in normal and NER-deficient human cells
Lima-Bessa, K. M. D., Armelini, M. G., Chiganças, V., Jacysyn, J. F., Amarante-Mendes, G. P., Sarasin, A., & Menck, C. F. M. (2008) · DNA Repair · doi:10.1016/j.dnarep.2007.11.003 · Cited by 87
“Moreover, GGR of CPDs is virtually absent in rodents [44,45].”
Background
Mitochondrial reactive oxygen species cause major oxidative mitochondrial DNA damages and repair pathways
Huang, Z., Chen, Y., & Zhang, Y. (2020) · Journal of Biosciences · doi:10.1007/s12038-020-00055-0 · Cited by 96
Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death
Dobson, A. W., Grishko, V. I., LeDoux, S. P., Kelley, M. R., Wilson, G. L., & Gillespie, M. N. (2002) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00443.2001 · Cited by 87
Transcription-coupled repair in RNA polymerase I-transcribed genes of yeast
Conconi, A., Bespalov, V. A., & Smerdon, M. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.022373099 · Cited by 87
Frequencies of synonymous substitutions in mammals are gene-specific and correlated with frequencies of nonsynonymous substitutions
Mouchiroud, D., Gautier, C., & Bernardi, G. (1995) · Journal of Molecular Evolution · doi:10.1007/bf00166602 · Cited by 87
Carcinogen susceptibility is regulated by genome architecture and predicts cancer mutagenesis
García-Nieto, P. E., Schwartz, E. K., King, D. A., Paulsen, J., Collas, P., Herrera, R. E., & Morrison, A. J. (2017) · The EMBO Journal · doi:10.15252/embj.201796717 · Cited by 91
“Furthermore, as previously introduced, several studies have shown that heterochromatin is more refractory to UV-induced repair than euchromatin (Bohr et al , 1985; Mellon et al , 1987; Hanawalt & Spivak, 2008; Hu et al , 2015; Adar et al , 2016).”
Historical and Current Highlights in Radiation Biology: Has Anything Important Been Learned by Irradiating Cells?
Bedford, J. S., & Dewey, W. C. (2002) · Radiation Research · doi:10.1667/0033-7587(2002)158[0251:hachir]2.0.co;2 · Cited by 87
RNA polymerase transcription factor IIH holoenzyme from yeast.
Svejstrup, J. Q., Feaver, W. J., LaPointe, J. W., & Kornberg, R. D. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)46892-5 · Cited by 85
Evolutionary consequences of nonrandom damage and repair of chromatin domains
Boulikas, T. (1992) · Journal of Molecular Evolution · doi:10.1007/bf00183227 · Cited by 84
A polymerase chain reaction-based method to detect cisplatin adducts in specific genes
Jennerwein, M. M., & Eastman, A. R. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.22.6209 · Cited by 84
The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH
Uchida, A., Sugasawa, K., Masutani, C., Dohmae, N., Araki, M., Yokoi, M., Ohkuma, Y., & Hanaoka, F. (2002) · DNA repair · doi:10.1016/s1568-7864(02)00031-9 · Cited by 84
A Nucleotide Excision Repair Master-Switch: p53 Regulated Coordinate Introduction of Global Genomic Repair Genes
Amundson, S. A., Patterson, A. R., Do, K. T., & Fornace, A. J. (2002) · Cancer Biology & Therapy · doi:10.4161/cbt.59 · Cited by 85
Transcription-repair coupling determines the strandedness of ultraviolet mutagenesis in Escherichia coli.
Oller, A. R., Fijałkowska, I. J., Dunn, R. L., & Schaaper, R. M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.22.11036 · Cited by 84
Differential role of transcription-coupled repair in UVB-induced G 2 arrest and apoptosis in mouse epidermis
van Oosten, M., Rebel, H., Friedberg, E. C., van Steeg, H., van der Horst, G. T. J., van Kranen, H. J., Westerman, A., van Zeeland, A. A., Mullenders, L. H. F., & de Gruijl, F. R. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.200226697 · Cited by 82
processingin vitroof an abasic site reacted with methoxyamine: a new assay for the detection of abasic sites formedin vivo
Rosa, S., Fortini, P., Karran, P., Bignami, M., & Eugenia Dogliotti, Y. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.20.5569 · Cited by 83
Blinded by the UV light: How the focus on transcription-coupled NER has distracted from understanding the mechanisms of Cockayne syndrome neurologic disease
Brooks, P. J. (2013) · DNA repair · doi:10.1016/j.dnarep.2013.04.018 · Cited by 84
“Such high levels are necessary to carry out the Southern blot based TC-NER assay, which requires roughly 1 adduct per 10 kb restriction fragment [33, 34].”
Background
Oxygen radical-induced mitochondrial DNA damage and repair in pulmonary vascular endothelial cell phenotypes
Grishko, V. I., Solomon, M., Wilson, G. L., LeDoux, S. P., & Gillespie, M. N. (2001) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.2001.280.6.l1300 · Cited by 83
Hypoxia promotes oxidative base modifications in the pulmonary artery endothelial cell VEGF gene
Grishko, V. I., Solomon, M., Breit, J. F., Killilea, D. W., LeDoux, S. P., Wilson, G. L., & Gillespie, M. N. (2001) · The FASEB Journal · doi:10.1096/fj.00-0755fje · Cited by 80
“Changes in equilibrium lesion density from baseline are estimated as -ln of the quotient of hybridization intensities in the treated and control bands, as described by others (26).”
Method
Revisiting the rodent repairadox
Hanawalt, P. C. (2001) · Environmental and Molecular Mutagenesis · doi:10.1002/em.1057 · Cited by 82
DNA strand break repair and neurodegeneration
Rulten, S. L., & Caldecott, K. W. (2013) · DNA repair · doi:10.1016/j.dnarep.2013.04.008 · Cited by 85
Nucleotide Excision Repair of Actively Transcribed Versus Nontranscribed DNA in Rat Hepatocytes: Effect of Age and Dietary Restriction
Guo, Z., Heydari, A. R., & Richardson, A. G. (1998) · Experimental Cell Research · doi:10.1006/excr.1998.4269 · Cited by 81
Transcription activities at 8-oxoG lesions in DNA
Larsen, E., Kwon, K.-R., Coin, F., Egly, J., & Klungland, A. (2004) · DNA repair · doi:10.1016/j.dnarep.2004.06.008 · Cited by 81
Cancer from the outside, aging from the inside: Mouse models to study the consequences of defective nucleotide excision repair
de Boer, J., & Hoeijmakers, J. H. J. (1999) · Biochimie · doi:10.1016/s0300-9084(99)80045-5 · Cited by 81
Proteolysis of a nucleotide excision repair protein by the 26S proteasome
Lommel, L., Ortolan, T. G., Chen, L., Madura, K., & Sweder, K. (2002) · Current Genetics · doi:10.1007/s00294-002-0332-9 · Cited by 80
“The incidence of CPDs in each strand of a specific restriction fragment was determined by previously developed methods ( Bohr et al. 1985; Mellon et al. 1987; Sweder and Hanawalt 1994).”
Method
Retinal Degeneration and Ionizing Radiation Hypersensitivity in a Mouse Model for Cockayne Syndrome
Gorgels, T. G. M. F., van der Pluijm, I., Brandt, R. M. C., Garinis, G. A., STEEG, H. V., van den Aardweg, G. J., Jansen, G. H., Ruijter, J. M., Bergen, A. A. B., Norren, D. V., et al. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.01037-06 · Cited by 82
Definition of a Short Region of XPG Necessary for TFIIH Interaction and Stable Recruitment to Sites of UV Damage
Thorel, F., Constantinou, A., Dunand-Sauthier, I., Nouspikel, T., Lalle, P., Raams, A., Jaspers, N. G. J., Vermeulen, W., Shivji, M. K. K., Wood, R. D., et al. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.24.10670-10680.2004 · Cited by 81
Glutathione and Drug Resistance
Schroder, C. P., Godwin, A. K., O'Dwyer, P. J., Tew, K. D., Hamilton, T. C., & Ozols, R. F. (1996) · Cancer Investigation · doi:10.3109/07357909609018891 · Cited by 79
Photochemical genotoxicity: principles and test methods
Brendler‐Schwaab, S., Czich, A., Epe, B., Gocke, E., Kaina, B., Müller, L., Pollet, D., & Utesch, D. (2003) · Mutation Research/Reviews in Mutation Research · doi:10.1016/s1383-5742(03)00052-8 · Cited by 78
Genome-wide transcription-coupled repair in Escherichia coli is mediated by the Mfd translocase
Adebalı, O., Chiou, Y., Hu, J., Sancar, A., & Selby, C. P. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1700230114 · Cited by 78
“The phenomenon was discovered first in mammalian cells (1, 2) and subsequently in Escherichia coli (3) and other organisms (4).”
Enhanced DDB2 expression protects mice from carcinogenic effects of chronic UV-B irradiation
Alekseev, S., Kool, H., Rebel, H., Fousteri, M., Moser, J., Backendorf, C., de Gruijl, F. R., Vrieling, H., & Mullenders, L. H. F. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-2295 · Cited by 77
Transcription affects the rate but not the extent of repair of cyclobutane pyrimidine dimers in the human adenosine deaminase gene.
Venema, J., Bartošová, Z., Natarajan, A. T., van Zeeland, A. A., & Mullenders, L. H. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50358-1 · Cited by 77
QUANTITATION OF PYRIMIDINE DIMERS BY IMMUNOSLOT BLOT FOLLOWING SUBLETHAL UV‐IRRADIATION OF HUMAN CELLS
Wani, A. A., D’Ambrosio, S. M., & Alvi, N. K. (1987) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1987.tb04798.x · Cited by 77
DNA–carcinogen interaction: covalent DNA-adducts of benzo(a)pyrene 7, 8-dihydrodiol 9, 10-epoxides studied by biochemical and biophysical techniques
Gräslund, A., & Jernström, B. (1989) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500003358 · Cited by 77
Quantification of Aminofluorene Adduct Formation and Repair in Defined DNA sequences in Mammalian Cells Using the UVRABC Nuclease
Tang, M. S., Bohr, V. A., Zhang, X. S., Pierce, J., & Hanawalt, P. C. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71700-6 · Cited by 77
Self-citation
A UV-Responsive G 2 Checkpoint in Rodent Cells
Orren, D. K., Petersen, L. N., & Bohr, V. A. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.7.3722 · Cited by 80
Self-citation
Untitled work
(2000) · PubMed · Cited by 76
The Saccharomyces cerevisiae histone acetyltransferase gcn5 has a role in the photoreactivation and nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers in the MFA2 gene
Teng, Y., Yu, Y., & Waters, R. (2002) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.5383 · Cited by 75
DNA Repair in Mammalian Cells
Eker, A. P. M., Quayle, C., Chaves, I., & van der Horst, G. T. J. (2009) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-009-8735-0 · Cited by 72
Transcription-coupled repair: A complex affair
Mellon, I. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2005.03.016 · Cited by 75
Yeast Nucleotide Excision Repair Proteins Rad2 and Rad4 Interact with RNA Polymerase II Basal Transcription Factor b (TFIIH)
Bardwell, A. J., Bardwell, L., Iyer, N., Svejstrup, J. Q., Feaver, W. J., Kornberg, R. D., & Friedberg, E. C. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.6.3569 · Cited by 75
DNA Repair in Mammalian Cells
Tornaletti, S. (2009) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-009-8738-x · Cited by 74
Mutator phenotype in cancer: Timing and perspectives
Bielas, J. H., & Loeb, L. A. (2005) · Environmental and Molecular Mutagenesis · doi:10.1002/em.20111 · Cited by 73
Damage control: DNA repair, transcription, and the ubiquitin–proteasome system
Daulny, A., & Tansey, W. P. (2009) · DNA repair · doi:10.1016/j.dnarep.2009.01.017 · Cited by 73
Methylated DNA-binding domain 1 and methylpurine–DNA glycosylase link transcriptional repression and DNA repair in chromatin
Watanabe, S., Ichimura, T., Fujita, N., Tsuruzoe, S., Ohki, I., Shirakawa, M., Kawasuji, M., & Nakao, M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2131819100 · Cited by 76
Structure and expression of the excision repair geneERCC6, involved in the human disorder Cockayne's syndrome group B
Troelstra, C., Hesen, W., Bootsma, D., & Hoeijmakers, J. H. J. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.3.419 · Cited by 74
Detection of DNA adducts at the DNA sequence level by ligation-mediated PCR
Pfeifer, G. P., Drouin, R., & Holmquist, G. P. (1993) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(93)90206-u · Cited by 74
Relationship between pyrimidine dimers, 6-4 photoproducts, repair synthesis and cell survival: Studies using cells from patients with trichothiodystrophy
Broughton, B. C., Lehmann, A. R., Harcourt, S. A., Arlett, C. F., Sarasin, A. R., Keijer, W. J., Beemer, F. A., Nairn, R., & Mitchell, D. L. (1990) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(90)90020-6 · Cited by 74
Repair and Transcription: Collision or collusion?
Hanawalt, P. C., Donahue, B. A., & Sweder, K. S. (1994) · Current Biology · doi:10.1016/s0960-9822(00)00112-3 · Cited by 98
Longevity and resistance to stress correlate with DNA repair capacity in Caenorhabditis elegans
Hyun, M., Lee, J., Lee, K., May, A., Bohr, V. A., & Ahn, B. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkm1161 · Cited by 71
“This assay measures the number of pyrimidine dimers in a target gene (VPS-45) using T4 endonuclease V (T4 endo V), making single-strand cleavages with high specificity for DNA pyrimidine dimers (29).”
BackgroundMethodInfluentialSelf-citation
Nucleotide excision repair and neurological diseases
Nouspikel, T. (2008) · DNA repair · doi:10.1016/j.dnarep.2008.03.015 · Cited by 73
Mitochondrial repair of 8-oxoguanine and changes with aging
Stevnsner, T., Thorslund, T., de Souza-Pinto, N. C., & Bohr, V. A. (2002) · Experimental Gerontology · doi:10.1016/s0531-5565(02)00142-0 · Cited by 73
Self-citation
Nucleotide excision repair syndromes: molecular basis and clinical symptoms
Bootsma, D., Weeda, G., Vermeulen, W., van Vuuren, H., Troelstra, C., van der Spek, P. J., & Hoeijmakers, J. H. J. (1995) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.1995.0012 · Cited by 73
DNA repair comes of age
Hanawalt, P. C. (1995) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(94)00061-a · Cited by 73

Authors: Bohr, V
Affiliations: Stanford University (US)
Corresponding author: Bohr, V
ORCID: Bohr, V
Venue: Cell
Publisher: Elsevier BV
Published: 1985-02
Volume: 40
Issue: 2
Pages: 359-369
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/0092-8674(85)90150-3
OpenAlex: W1984353960
Cited by: 1,237
References: 34
Citations by year: 2026: 11 · 2025: 17 · 2024: 15 · 2023: 14 · 2022: 12 · 2021: 17 · 2020: 18 · 2019: 11 · 2018: 18 · 2017: 13 · 2016: 23 · 2015: 9 · 2014: 24 · 2013: 24 · 2012: 22
FWCI: 15.13
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: National Institute of General Medical Sciences (GM 09901)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
Keywords: DNA repair, pyrimidine dimers, dihydrofolate reductase gene, UV-induced DNA damage, UV endonuclease, UV resistance, CHO cells, mutagenesis, cell survival
MeSH terms: Animals, Base Sequence, Cell Line, Cell Survival, Cricetulus, DNA Repair, Endodeoxyribonucleases, Female, Cricetinae, Multienzyme Complexes, N-Glycosyl Hydrolases, Pyrimidine Dimers, Tetrahydrofolate Dehydrogenase

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks