Cited by 172Seaweed-derived Bioactive CompoundsPolysaccharides Composition and ApplicationsPolysaccharides and Plant Cell Walls

In vitro antiviral activities of sulfated polysaccharides from a marine microalga (Cochlodinium polykrikoides) against human immunodeficiency virus and other enveloped viruses

Hasui, Masahiko, Matsuda, Masahiro, Okutani, Koichi, Shigeta, Shiro·1995·International Journal of Biological Macromolecules

Abstract

A marine microalga, Cochlodinium polykrikoides, produces extracellular sulfated polysaccharides. Isolation and purification of the polysaccharides were accomplished by precipitation with ethanol and Cetavlon, followed by DEAE-cellulose column chromatography (polysaccharides A1 and A2). These polysaccharides, which were homogeneous when analysed by both ultracentrifugal and electrophoretic methods, were composed of mannose, galactose, glucose and uronic acid, together with sulfate groups (S = 7-8% w/w). Both A1 and A2 inhibited the cytopathic effect of influenza virus types A and B in MDCK cells, that of respiratory syncytial virus types A and B in HEp-2 cells, that of human immunodeficiency virus type 1 in MT-4 cells; and, except A1 for herpes simplex virus type 1 and A2 for parainfluenza virus type 2 in HMV-2 cells, the cochlodinium polysaccharides showed no antiviral activity against parainfluenza virus types 2 and 3, measles virus, mumps virus or herpes simplex virus type 1 in HMV-2 cells. No cytotoxicity for host cells was observed with these polysaccharides at a concentration of 100 micrograms ml-1. Inhibitory effects on various viruses were achieved at concentrations that were not markedly inhibitory to the blood coagulation process.

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Crossref · 10.1016/0141-8130(95)98157-t

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Authors
Hasui, M., Matsuda, M., Okutani, K., & Shigeta, S.
Affiliations
Kagawa University (JP)
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
1995-01
Volume
17
Issue
5
Pages
293-297
ISSN
0141-8130 (ISSN-L)
OpenAlex
W2047601275
Cited by
172
References
14
Citations by year
2026: 4 · 2025: 5 · 2024: 10 · 2023: 6 · 2022: 24 · 2021: 18 · 2020: 11 · 2019: 5 · 2018: 8 · 2017: 5 · 2016: 5 · 2015: 7 · 2014: 5 · 2013: 7 · 2012: 6
FWCI
3.65
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
sulfated polysaccharides, Cochlodinium polykrikoides, marine microalgae, antiviral activity, HIV-1, enveloped viruses, influenza virus, respiratory syncytial virus, herpes simplex virus type 1, parainfluenza virus type 2, uronic acid, polysaccharide purification, cytopathic effect inhibition, marine natural products
MeSH terms
Animals, Antiviral Agents, Cell Division, Cell Line, Chromatography, Drug Evaluation, Preclinical, Humans, Kidney, Laryngeal Neoplasms, Influenza A virus, Polysaccharides, Respiratory Syncytial Viruses, Spectrophotometry, Infrared, Sulfates, Tumor Cells, Cultured, Virus Replication, HIV-1, Simplexvirus, Eukaryota

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