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Cited by 290Antifungal resistance and susceptibilityBacterial Identification and Susceptibility TestingFungal Infections and Studies

Lysis-centrifugation blood cultures in the detection of tissue-proven invasive candidiasis. Disseminated versus single-organ infection

Berenguer, Juan, Buck, Michael, Witebsky, Frank, Stock, Frida, Pizzo, Philip A., Walsh, Thomas J.·1993·Diagnostic Microbiology and Infectious Disease
DOI 10.1016/0732-8893(93)90020-8
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Abstract

Several studies have demonstrated significantly higher frequency and more rapid detection of candidemia with blood culture methods performed by lysis-centrifugation (LC) in comparison with other techniques. Little is known, however, about the ability of LC blood culture methods to detect tissue-proven invasive candidiasis. We therefore investigated the sensitivity of LC blood cultures in the detection of tissue-proven invasive candidiasis. Between 1985 and 1991, invasive candidiasis was detected in 41 (5.1%) of 803 autopsies at the Clinical Center of the National Institutes of Health (Bethesda, MD, USA). Cases were classified as single-organ (SO) candidiasis (n = 20) and as disseminated candidiasis (DI) (n = 21). Patients with DI were more likely than those with SO to have a hematologic malignancy (71% vs 15%, P < 0.001) and to have gastrointestinal mucosal candidiasis (76% vs 25%, P = 0.003). LC detected fungemia in 16 (43%) of all 37 cases with blood cultures. When analyzed by classification, Candida spp. were isolated from blood in 11 (58%) of 19 patients with DI and in five (28%) of 18 patients with SO (P = 0.13). When analyzed by number of organs infected, blood cultures were positive in seven (78%) of nine patients with > 3 organs infected by Candida in comparison to five (28%) of 18 patients with one organ infected (P = 0.024). The mean recovery time for Candida in blood cultures was 2.6 days in DI and 3.2 days in SO (P = 0.017). There was no difference in colonies of organisms per LC tube between patients with DI and those with SO.(ABSTRACT TRUNCATED AT 250 WORDS)

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  • The gold standard diagnostic test for invasive candidiasis has been isolation of the organism in blood culture, but blood culture for Candida is insensitive.

    “In addition, the gold standard diagnostic test for IC has been isolation of the organism in blood culture, however, blood culture for Candida is insensitive.[52][53][54][55]”

    From Fungal Sepsis: Optimizing Antifungal Therapy in the Critical Care Setting (2011) · cites this paper as [54]

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Authors
Berenguer, J., Buck, M., Witebsky, F., Stock, F., Pizzo, P. A., & Walsh, T. J.
Affiliations
National Cancer Institute (US)
ORCID
Venue
Diagnostic Microbiology and Infectious Disease
Publisher
Elsevier BV
Published
1993-08
Volume
17
Issue
2
Pages
103-109
ISSN
0732-8893 (ISSN-L)
DOI
10.1016/0732-8893(93)90020-8
OpenAlex
W2093767199
Cited by
290
References
21
Citations by year
2026: 2 · 2025: 6 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 3 · 2020: 4 · 2019: 9 · 2018: 6 · 2017: 8 · 2016: 9 · 2015: 9 · 2014: 17 · 2013: 11 · 2012: 22
FWCI
4.08
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Diagnostic Microbiology and Infectious Disease (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
invasive candidiasis, systemic candidiasis, candidemia, fungemia, autopsy findings, Candida detection, hematologic malignancies, recovery time
MeSH terms
Adolescent, Adult, Aged, Candidiasis, Centrifugation, Culture Media, Digestive System, Female, Humans, Kidney, Lung, Lung Diseases, Fungal, Male, Middle Aged, Sensitivity and Specificity, Colony Count, Microbial, Chi-Square Distribution, Fungemia

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