Calcium-induced actin depolymerization reduces NMDA channel activity
Abstract
Actin filaments are highly concentrated in postsynaptic densities at central excitatory synapses, but their influence on postsynaptic glutamate receptors is unknown. We tested whether actin depolymerization influences NMDA channel activity in whole-cell recording on cultured hippocampal neurons. The ATP- and calcium-dependent rundown of NMDA channels was prevented when actin depolymerization was blocked by phalloidin. Rundown of AMPA/kainate receptors was unaffected by phalloidin. Cytochalasins, which enhance actin-ATP hydrolysis, induced NMDA channel rundown, whereas taxol or colchicine, which stabilize or disrupt microtubule assembly, had no effect. Protease inhibitors also had no effect. Our results suggest that calcium and ATP can influence NMDA channel activity by altering the state of actin polymerization and are consistent with a proposed model in which actin filaments compartmentalize a channel regulatory protein.
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Both calcineurin and F-actin are involved in controlling the efficacy of NMDA receptors.
“Both calcineurin and F-actin are involved in controlling the efficacy of NMDA receptors (Rosenmund and Westbrook, 1993;Lieberman and Mody, 1994;Tong et al., 1995).”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Rosenmund and Westbrook, 1993
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- Authors
- Rosenmund, C., & Westbrook, G. L.
- Affiliations
- Oregon Health & Science University (US)
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1993-05
- Volume
- 10
- Issue
- 5
- Pages
- 805-814
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2027646146
- Cited by
- 503
- References
- 0
- Citations by year
- 2025: 2 · 2024: 8 · 2023: 3 · 2022: 4 · 2021: 8 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 6 · 2014: 14 · 2013: 10 · 2012: 7
- FWCI
- 10.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service
- National Institute of Mental Health (MH-46613)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Keywords
- actin depolymerization, NMDA receptor, calcium, ATP, postsynaptic density, non-NMDA receptors, phalloidin, cytochalasin, actin polymerization, glutamate receptors, hippocampal neurons, channel rundown
- MeSH terms
- Actins, Adenosine Triphosphate, Animals, Calcium, Cells, Cultured, Cytochalasins, Electric Conductivity, Hippocampus, Ion Channels, Neurons, Phalloidine, Receptors, N-Methyl-D-Aspartate, Paclitaxel, Rats
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