The DOI matches its Crossref record; 57 of the 58 references it cites were found in a registry or an index.
Cloning and localization of a conventional kinesin motor expressed exclusively in neurons
Abstract
Kinesin is a microtubule-based motor protein involved in organelle transport in neuronal and nonneuronal cells. Although a single kinesin motor has been thought to serve all cell types, we document here that neurons express a second conventional kinesin heavy chain (nKHC) that is 65% identical in amino acid sequence to the ubiquitously expressed kinesin heavy chain (uKHC). By preparing antibodies which distinguish between the two KHCs, we demonstrate that nKHC is a nucleotide-dependent microtubule-binding protein which partially cofractionates with membrane organelles. Immunolocalization experiments show that nKHC is distributed throughout the CNS but is highly enriched in subsets of neurons. In hippocampal neurons in culture, uKHC is distributed uniformly throughout the neuron, whereas nKHC is selectively concentrated in the cell body. These results demonstrate that mammalian neuronal tissue contains two conventional kinesin motors which may serve distinct functions in microtubule-based transport.
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“In mammals, the polypeptide components of kinesin-I are encoded by three KHC genes, KIF5A, KIF5B, and KIF5C (Gudkov et al. 1994; Niclas et al. 1994; Nakagawa et al. 1997; Xia et al. 1998), and three KLC genes, KLC1, KLC2, and KLC3 (Lamerdin et al. 1996; Rahman et al. 1998).”
From Defective kinesin heavy chain behavior in mouse kinesin light chain mutants (1999) · cites this paper as Niclas et al. 1994
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Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport
Hirokawa, N. (1998) · Science · doi:10.1126/science.279.5350.519 · Cited by 1,689
All kinesin superfamily protein, KIF, genes in mouse and human
Miki, H., Setou, M., Kaneshiro, K., & Hirokawa, N. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111145398 · Cited by 712
Okada, Y., Yamazaki, H., Sekine‐Aizawa, Y., & Hirokawa, N. (1995) · Cell · doi:10.1016/0092-8674(95)90538-3 · Cited by 679
Tanaka, Y., Kanai, Y., Okada, Y., Nonaka, S., Takeda, S., Harada, A., & Hirokawa, N. (1998) · Cell · doi:10.1016/s0092-8674(00)81459-2 · Cited by 654
Microtubule-Based Transport Systems in Neurons: The Roles of Kinesins and Dyneins
Goldstein, L. S. B., & Yang, Z. (2000) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.23.1.39 · Cited by 563
Metadata
- Authors
- Niclas, J., Navone, F., Hom-Booker, N., & Vale, R. D.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1994-05
- Volume
- 12
- Issue
- 5
- Pages
- 1059-1072
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2081395783
- Cited by
- 153
- References
- 58
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 1 · 2012: 4
- FWCI
- 4.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM38499)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- kinesin heavy chain, microtubule transport, hippocampal neurons, organelle transport, microtubule-binding protein, immunolocalization
- MeSH terms
- Aging, Amino Acid Sequence, Animals, Animals, Newborn, Brain, Cells, Cultured, Cloning, Molecular, Drosophila, Fluorescent Antibody Technique, HeLa Cells, Hippocampus, Humans, Molecular Sequence Data, Neurons, Poly A, Recombinant Fusion Proteins, RNA, RNA, Messenger, Sciatic Nerve, Blotting, Northern, Organelles, Gene Expression, Kinesins, Sequence Homology, Amino Acid, Macromolecular Substances, Rats
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