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Cited by 153Microtubule and mitosis dynamicsHereditary Neurological DisordersGenetic and Kidney Cyst Diseases

The DOI matches its Crossref record; 57 of the 58 references it cites were found in a registry or an index.

Cloning and localization of a conventional kinesin motor expressed exclusively in neurons

Niclas, Joshua, Navone, Francesca, Hom-Booker, Nora, Vale, Ronald D.·1994·Neuron
DOI 10.1016/0896-6273(94)90314-x
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Abstract

Kinesin is a microtubule-based motor protein involved in organelle transport in neuronal and nonneuronal cells. Although a single kinesin motor has been thought to serve all cell types, we document here that neurons express a second conventional kinesin heavy chain (nKHC) that is 65% identical in amino acid sequence to the ubiquitously expressed kinesin heavy chain (uKHC). By preparing antibodies which distinguish between the two KHCs, we demonstrate that nKHC is a nucleotide-dependent microtubule-binding protein which partially cofractionates with membrane organelles. Immunolocalization experiments show that nKHC is distributed throughout the CNS but is highly enriched in subsets of neurons. In hippocampal neurons in culture, uKHC is distributed uniformly throughout the neuron, whereas nKHC is selectively concentrated in the cell body. These results demonstrate that mammalian neuronal tissue contains two conventional kinesin motors which may serve distinct functions in microtubule-based transport.

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Crossref · 10.1016/0896-6273(94)90314-x

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Metadata

Authors
Niclas, J., Navone, F., Hom-Booker, N., & Vale, R. D.
Affiliations
University of California, San Francisco (US)
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
1994-05
Volume
12
Issue
5
Pages
1059-1072
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/0896-6273(94)90314-x
OpenAlex
W2081395783
Cited by
153
References
58
Citations by year
2025: 4 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 1 · 2012: 4
FWCI
4.29
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM38499)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
kinesin heavy chain, microtubule transport, hippocampal neurons, organelle transport, microtubule-binding protein, immunolocalization
MeSH terms
Aging, Amino Acid Sequence, Animals, Animals, Newborn, Brain, Cells, Cultured, Cloning, Molecular, Drosophila, Fluorescent Antibody Technique, HeLa Cells, Hippocampus, Humans, Molecular Sequence Data, Neurons, Poly A, Recombinant Fusion Proteins, RNA, RNA, Messenger, Sciatic Nerve, Blotting, Northern, Organelles, Gene Expression, Kinesins, Sequence Homology, Amino Acid, Macromolecular Substances, Rats

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