Cited by 56Electrochemical sensors and biosensorsIonic liquids properties and applicationsElectrochemical Analysis and Applications

Electrical double layer modulation of hybrid room temperature ionic liquid/aqueous buffer interface for enhanced sweat based biosensing

Jagannath, Badrinath, Muthukumar, Sriram, Prasad, Shalini·2018·Analytica Chimica Acta

Abstract

We have investigated the role of kosmotropic anionic moieties and chaotropic cationic moieties of room temperature hydrophilic ionic liquids in enhancing the biosensing performance of affinity based immunochemical biosensors in human sweat. Two ionic liquids, 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM[BF 4]) and choline dihydrogen phosphate (Choline[DHP]) were investigated in this study with Choline[DHP] being more kosmotropic in nature having a more protein stabilizing effect based on the hofmeister series. Non-faradaic interfacial charge transfer has been employed as the mechanism for evaluating the formation and the biosensing of capture probe antibodies in room temperature ionic liquids (RTILs)/aqueous human sweat interface. The charge of the ionic moieties were utilized to form compact electrical double layers around the antibodies for enhancing the stability of the antibody capture probes, which was evaluated through zeta potential measurements. The zeta potential measurements indicated stability of antibodies due to electrostatic repulsion of the RTIL charged moieties encompassing the antibodies, thus preventing any aggregation. Here, we report for the first time of non-faradaic electrochemical impedance spectroscopy equivalent circuit model analysis for analyzing and interpreting affinity based biosensing at hybrid electrode/ionic liquid-aqueous sweat buffer interface guided by the choice of the ionic liquid. Interleukin-6 (IL-6) and cortisol two commonly occurring biomarkers in human sweat were evaluated using this method. The limit of detection (LOD) obtained using both ionic liquids for IL-6 was 0.2 pg mL-1 with cross-reactivity studies indicating better performance of IL-6 detection using Choline[DHP] and no response to cross-reactive molecule. The LOD of 0.1 ng/mL was achieved for cortisol and the cross-reactivity studies indicated that cortisol antibody in BMIM[BF 4] did not show any signal response to cross-reactive molecules. Furthermore, improved sensitivity and LOD was achieved using ionic liquids as compared to capture probes in aqueous buffer.

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Crossref · 10.1016/j.aca.2018.02.013

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Authors
Jagannath, B., Muthukumar, S., & Prasad, S.
Affiliations
The University of Texas at Dallas (US)
Corresponding author
Prasad, Shalini
Venue
Analytica Chimica Acta
Publisher
Elsevier BV
Published
2018-08
Volume
1016
Pages
29-39
ISSN
0003-2670 (ISSN-L)
OpenAlex
W2789738698
Cited by
56
References
34
Citations by year
2026: 2 · 2025: 8 · 2024: 9 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 8 · 2019: 5 · 2018: 4
FWCI
2.39
Type
journal article
Language
en
Open access
closed
Locations
  • Analytica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
Funders
University of Texas at Dallas
Topics
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
  • Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
  • Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
Keywords
room temperature ionic liquids, electric double layer, 1-butyl-3-methylimidazolium tetrafluoroborate, choline dihydrogen phosphate, antibody stability, interleukin-6 detection, cortisol detection, sweat biomarkers, antibody aggregation
MeSH terms
Buffers, Electricity, Humans, Sweat, Temperature, Water, Biosensing Techniques, Ionic Liquids, Hydrophobic and Hydrophilic Interactions, Dielectric Spectroscopy

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