A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
Open accessAbstract
Successful commercialization of wearable diagnostic sensors necessitates stability in detection of analytes over prolonged and continuous exposure to sweat. Challenges are primarily in ensuring target disease specific small analytes (i.e. metabolites, proteins, etc.) stability in complex sweat buffer with varying pH levels and composition over time. We present a facile approach to address these challenges using RTILs with antibody functionalized sensors on nanoporous, flexible polymer membranes. Temporal studies were performed using both infrared spectroscopic, dynamic light scattering, and impedimetric spectroscopy to demonstrate stability in detection of analytes, Interleukin-6 (IL-6) and Cortisol, from human sweat in RTILs. Temporal stability in sensor performance was performed as follows: (a) detection of target analytes after 0, 24, 48, 96, and 168 hours post-antibody sensor functionalization; and (b) continuous detection of target analytes post-antibody sensor functionalization. Limit of detection of IL-6 in human sweat was 0.2 pg/mL for 0–24 hours and 2 pg/mL for 24–48 hours post-antibody sensor functionalization. Continuous detection of IL-6 over 0.2–200 pg/mL in human sweat was demonstrated for a period of 10 hours post-antibody sensor functionalization. Furthermore, combinatorial detection of IL-6 and Cortisol in human sweat was established with minimal cross-talk for 0–48 hours post-antibody sensor functionalization.
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A wearable immunosensor for detecting cortisol and interleukin 6 in sweat has diagnostic potential.
“For instance, a wearable immunosensor for detecting cortisol and interleukin 6 (IL-6) in sweat has diagnostic potential (Fig. 3g)43.”
From Wearable biosensors for healthcare monitoring (2019) · cites this paper as [43]
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- Authors
- Munje, R. D., Muthukumar, S., Jagannath, B., & Prasad, S.
- Affiliations
- The University of Texas at Dallas (US)
- Corresponding author
- Prasad, Shalini
- Venue
- Scientific Reports
- Published
- 2017-05-16
- Volume
- 7
- Issue
- 1
- Pages
- 1950-1950
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5434046
- OpenAlex
- W2612250156
- Cited by
- 149
- References
- 46
- Citations by year
- 2026: 13 · 2025: 16 · 2024: 24 · 2023: 16 · 2022: 23 · 2021: 19 · 2020: 12 · 2019: 12 · 2018: 12 · 2017: 2
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemical Engineering › Catalysis › Ionic liquids properties and applications
- Physical Sciences › Engineering › Biomedical Engineering › Advanced Sensor and Energy Harvesting Materials
- Keywords
- wearable diagnostics, room temperature ionic liquids, interleukin-6 detection, cortisol detection, nanoporous membranes, sensor stability
- MeSH terms
- Dynamic Light Scattering, Wearable Electronic Devices, Humans, Hydrocortisone, Nylons, Sweat, Temperature, Zinc Oxide, Biosensing Techniques, Porosity, Spectroscopy, Fourier Transform Infrared, Ionic Liquids
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