Flexible nanoporous tunable electrical double layer biosensors for sweat diagnostics
Open accessAbstract
An ultra-sensitive and highly specific electrical double layer (EDL) modulated biosensor, using nanoporous flexible substrates for wearable diagnostics is demonstrated with the detection of the stress biomarker cortisol in synthetic and human sweat. Zinc oxide thin film was used as active region in contact with the liquid i.e. synthetic and human sweat containing the biomolecules. Cortisol detection in sweat was accomplished by measuring and quantifying impedance changes due to modulation of the double layer capacitance within the electrical double layer through the application of a low orthogonally directed alternating current (AC) electric field. The EDL formed at the liquid-semiconductor interface was amplified in the presence of the nanoporous flexible substrate allowing for measuring the changes in the alternating current impedance signal due to the antibody-hormone interactions at diagnostically relevant concentrations. High sensitivity of detection of 1 pg/mL or 2.75 pmol cortisol in synthetic sweat and 1 ng/mL in human sweat is demonstrated with these novel biosensors. Specificity in synthetic sweat was demonstrated using a cytokine IL-1β. Cortisol detection in human sweat was demonstrated over a concentration range from 10–200 ng/mL.
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Self-citation
- Authors
- Munje, R. D., Muthukumar, S., Panneer Selvam, A., & Prasad, S.
- Affiliations
- The University of Texas at Dallas (US)
- Corresponding author
- Prasad, Shalini
- Venue
- Scientific Reports
- Published
- 2015-09-30
- Volume
- 5
- Issue
- 1
- Pages
- 14586-14586
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4588585
- OpenAlex
- W2412316727
- Cited by
- 138
- References
- 27
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 17 · 2023: 11 · 2022: 16 · 2021: 14 · 2020: 15 · 2019: 14 · 2018: 25 · 2017: 10 · 2016: 4
- FWCI
- 6.26
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- University of Texas at Dallas
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
- Keywords
- wearable diagnostics, cortisol detection, zinc oxide thin film, impedance sensing, alternating current impedance, human sweat, synthetic sweat, stress biomarkers
- MeSH terms
- Electronics, Humans, Sweat, Zinc Oxide, Reproducibility of Results, Biosensing Techniques, Porosity, Electric Impedance, Nanotechnology, Nanostructures
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