The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells
Open accessAbstract
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration. However, little is known about the suitability of ACPCs for tissue engineering, especially in combination with biomaterials. This study aimed to investigate the potential of ACPCs in hydrogels for cartilage regeneration and biofabrication, and to evaluate their ability for zone-specific matrix production. Gelatin methacryloyl (gelMA)-based hydrogels were used to culture ACPCs, bone marrow mesenchymal stromal cells (MSCs) and chondrocytes, and as bioinks for printing. Our data shows ACPCs outperformed chondrocytes in terms of neo-cartilage production and unlike MSCs, ACPCs had the lowest gene expression levels of hypertrophy marker collagen type X, and the highest expression of PRG4, a key factor in joint lubrication. Co-cultures of the cell types in multi-compartment hydrogels allowed generating constructs with a layered distribution of collagens and glycosaminoglycans. By combining ACPC- and MSC-laden bioinks, a bioprinted model of articular cartilage was generated, consisting of defined superficial and deep regions, each with distinct cellular and extracellular matrix composition. Taken together, these results provide important information for the use of ACPC-laden hydrogels in regenerative medicine, and pave the way to the biofabrication of 3 D constructs with multiple cell types for cartilage regeneration or in vitro tissue models.
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A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
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3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs
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“Unmodified gelMA does not exhibit a marked yield stress, and is therefore difficult to print with high shape fidelity [9,60,61].”
The surface of articular cartilage contains a progenitor cell population
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Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair
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Bioinks for 3D bioprinting: an overview
Gungor-Ozkerim, P. S., Inci, I., Zhang, Y. S., Khademhosseini, A., & Dokmeci, M. R. (2018) · Biomaterials Science · doi:10.1039/c7bm00765e · Cited by 1,428
Self-citationPrintability and shape fidelity of bioinks in 3D bioprinting
Schwab, A., Levato, R., D’Este, M., Piluso, S., Eglin, D., & Malda, J. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00084 · Cited by 1,359
Self-citationProgress in 3D bioprinting technology for tissue/organ regenerative engineering
Matai, I., Kaur, G., Seyedsalehi, A., McClinton, A., & Laurencin, C. T. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119536 · Cited by 1,156
Recent trends in bioinks for 3D printing
Gopinathan, J., & Noh, I. (2018) · Biomaterials Research · doi:10.1186/s40824-018-0122-1 · Cited by 846
Biofabrication strategies for 3D in vitro models and regenerative medicine
Moroni, L., Burdick, J. A., Highley, C., Lee, S. J., Morimoto, Y., Takeuchi, S., & Yoo, J. J. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0006-y · Cited by 753
Metadata
- Authors
- Levato, R., Webb, W. R., Otto, I. A., Mensinga, A., Zhang, Y., van Rijen, M., van Weeren, R., Khan, I. M., & Malda, J.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 61
- Pages
- 41-53
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2744149796
- Cited by
- 326
- References
- 62
- FWCI
- 14.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Levato, R., Webb, W. R., Otto, I. A., Mensinga, A., Zhang, Y., van Rijen, M., van Weeren, R., Khan, I. M., & Malda, J. (2017). The bio in the ink: cartilage regeneration with bioprintable hydrogels and articular cartilage-derived progenitor cells. Acta Biomaterialia. https://doi.org/10.1016/j.actbio.2017.08.005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
