Cell-laden microengineered gelatin methacrylate hydrogels
Open accessAbstract
The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials are not cell-responsive and have limited biodegradability. Here, we demonstrate gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. Cells readily bound to, proliferated, elongated, and migrated both when seeded on micropatterned GelMA substrates as well as when encapsulated in microfabricated GelMA hydrogels. The hydration and mechanical properties of GelMA were demonstrated to be tunable for various applications through modification of the methacrylation degree and gel concentration. The pattern fidelity and resolution of GelMA were high and it could be patterned to create perfusable microfluidic channels. Furthermore, GelMA micropatterns could be used to create cellular micropatterns for in vitro cell studies or 3D microtissue fabrication. These data suggest that GelMA hydrogels could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, or for other applications that require cell-responsive microengineered hydrogels.
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“During the synthesis of GelMA, gelatin undergoes modification through the introduction of methacrylic acid (MA), substituting some amino groups in gelatin with methacrylate groups [58].”
- Authors
- Nichol, J. W., Koshy, S. T., Bae, H., Hwang, C. M., Yamanlar, S., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- Corresponding author
- Khademhosseini, Ali
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2010-07
- Volume
- 31
- Issue
- 21
- Pages
- 5536-5544
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2878615
- OpenAlex
- W1976842528
- Cited by
- 2,377
- References
- 58
- Citations by year
- 2026: 129 · 2025: 173 · 2024: 192 · 2023: 206 · 2022: 207 · 2021: 241 · 2020: 220 · 2019: 198 · 2018: 169 · 2017: 152 · 2016: 166 · 2015: 99 · 2014: 67 · 2013: 79 · 2012: 42
- FWCI
- 23.30
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL092836, R01HL092836)
- National Institute of Biomedical Imaging and Bioengineering (R21EB009196, R01EB008392)
- National Institute of Dental and Craniofacial Research (DE019024, RL1DE019024)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- gelatin methacryloyl, cell-laden hydrogels, photopatterning, micropatterning, microfluidics, hydrogel hydration, hydrogel mechanical properties, 3D cell encapsulation, cell migration, biodegradable hydrogels
- MeSH terms
- Biocompatible Materials, Cell Adhesion, Cell Movement, Cells, Cultured, Gelatin, Humans, Materials Testing, Methacrylates, Surface Properties, Molecular Structure, Hydrogels, Tissue Engineering, Microfluidics, Cell Proliferation, Tissue Scaffolds
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