Synergistic effect of Glomus mosseae and nitrogen fixing Bacillus subtilis strain Daz26 on artemisinin content in Artemisia annua L.
Abstract
Artemisia annua L. (Asteraceae) is an important medicinal plant whose secondary metabolite artemisinin is used for the treatment of cerebral malaria. A study was undertaken to determine the effect of arbuscular mycorrhizal (AM) fungi, Glomus mosseae, Glomus aggregatum, Glomus fasciculatum, Glomus intraradices and two free living nitrogen fixing bacteria (NFB) (identified as Bacillus subtilis and Stenotrophomonas spp. through 16S rRNA gene sequence analysis), inoculated alone or in combinations on the biomass, nutrient uptake, and content of artemisinin in A. annua under glass house conditions. Various parameters like plant height, total plant biomass, NPK content, leaf yield, mycorrhizal colonization, bacterial population in rhizosphere, phosphatase activity and artemisinin content were determined and found to vary with different treatments. Among all the treatments, plants inoculated with G. mosseae+B. subtilis performed better than any other treatment or uninoculated control plants. The results of the experiment clearly indicated the compatibility and synergy between G. mosseae and B. subtilis and suggested the use of this microbial consortium in A. annua for enhancing growth, biomass yield, and the content and yield of artemisinin.
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Combinations of G. mosseae + Bacillus subtilis and G. fasciculatum + Bacillus subtilis resulted in a significant increase in artemisinin content, 26.2 and 24.6%, respectively, in A. annua.
“Awasthi et al. (2011) used four species of AM fungi Glomus, namely G . mosseae , G. aggregatum , G. intraradices and G. fasciculatum, nitrogen fixing bacteria namely Stenotrophomonas spp. and Bacillus subtilis , and showed that combinations of G. mosseae + Bacillus subtilis and G. fasciculatum + Bacillus subtilis resulted in a significant increase in artemisinin content, 26.2 and 24.6%, respective”
From Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches (2021) · cites this paper as Awasthi et al. 2011
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- Authors
- Awasthi, A., Bharti, N., Nair, P., Singh, R., Shukla, A. K., Gupta, M. M., Darokar, M. P., & Kalra, A.
- Affiliations
- Central Institute of Medicinal and Aromatic Plants (IN)
- Corresponding author
- Kalra, Alok
- ORCID
- Venue
- Applied Soil Ecology
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 49
- Pages
- 125-130
- ISSN
- 0929-1393 (ISSN-L)
- OpenAlex
- W1994346490
- Cited by
- 127
- References
- 31
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 9 · 2023: 15 · 2022: 4 · 2021: 15 · 2020: 10 · 2019: 5 · 2018: 10 · 2017: 5 · 2016: 14 · 2015: 6 · 2014: 8 · 2013: 9 · 2012: 6
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Applied Soil Ecology (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Central Institute of Medicinal and Aromatic Plants
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycorrhizal Fungi and Plant Interactions
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
- Keywords
- Artemisia annua, artemisinin, Glomus mosseae, Bacillus subtilis, arbuscular mycorrhizal fungi, nitrogen-fixing bacteria, microbial consortia, synergistic effect, secondary metabolite production, plant growth promotion, nutrient uptake, mycorrhizal colonization, phosphatase activity, medicinal plants
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