Cited by 123Bone Metabolism and DiseasesBone health and osteoporosis researchBone and Dental Protein Studies

Death of osteocytes turns off the inhibition of osteoclasts and triggers local bone resorption

Gu, Guoliang, Mulari, Mika, Peng, Zhiqi, Hentunen, Teuvo A., Väänänen, H. Kalervo·2005·Biochemical and Biophysical Research Communications

Abstract

Osteocytes have been suggested to play a role in the regulation of bone resorption, although their effect on bone turnover has remained controversial. In order to study this open question, we developed an organ culture system based on isolated rat calvaria, where the osteocyte viability and its effect on osteoclastic bone resorption can be monitored. Our results suggest that osteocytes are constitutively negative regulators of osteoclastic activity. Osteoclasts, which were cultured on calvarial slices with living osteocytes inside, failed to form actin rings which are the hallmarks of resorbing cells. A similar inhibitory effect was also achieved by the conditioned medium obtained from calvarial organ culture, suggesting that living osteocytes produce yet unrecognized osteoclast inhibitors. On the contrary, when osteocyte apoptosis was induced, this inhibitory effect disappeared and strong osteoclastic bone resorption activity was observed. Thus, local apoptosis of osteocytes may play a major role in triggering local bone remodeling.

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Crossref · 10.1016/j.bbrc.2005.06.211

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Authors
Gu, G., Mulari, M., Peng, Z., Hentunen, T. A., & Väänänen, H. K.
Affiliations
University of Turku (FI)
Corresponding author
Väänänen, H. Kalervo
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2005-10
Volume
335
Issue
4
Pages
1095-1101
ISSN
0006-291X (ISSN-L)
OpenAlex
W2087877947
Cited by
123
References
34
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 7 · 2013: 10 · 2012: 12
FWCI
3.01
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
osteocytes, osteoclasts, bone resorption, osteocyte apoptosis, osteoclast inhibition, bone remodeling, actin rings, osteoclast inhibitors, conditioned medium, bone turnover
MeSH terms
Animals, Bone Resorption, Cell Communication, Cells, Cultured, Osteoclasts, Osteocytes, Skull, Women, Rats, Sprague-Dawley, Apoptosis, Coculture Techniques, Rats

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