Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies
Abstract
In the United States, more than 2600 kidneys are discarded annually, from the total number of kidneys procured for transplant. We hypothesized that this organ pool may be used as a platform for renal bioengineering and regeneration research. We previously showed that decellularization of porcine kidneys yields renal extracellular matrix (ECM) scaffolds that maintain their basic components, support cell growth and welfare in vitro and in vivo, and show an intact vasculature that, when such scaffolds are implanted in vivo, is able to sustain physiological blood pressure. The purpose of the current study was to test if the same strategy can be applied to discarded human kidneys in order to obtain human renal ECM scaffolds. The results show that the sodium dodecylsulfate-based decellularization protocol completely cleared the cellular compartment in these kidneys, while the innate ECM framework retained its architecture and biochemical properties. Samples of human renal ECM scaffolds stimulated angiogenesis in a chick chorioallantoic membrane assay. Importantly, the innate vascular network in the human renal ECM scaffolds retained its compliance. Collectively, these results indicate that discarded human kidneys are a suitable source of renal scaffolds and their use for tissue engineering applications may be more clinically applicable than kidneys derived from animals.
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Decellularization approaches have been demonstrated for human kidney, murine kidney, murine heart, human and porcine lung, and porcine testicular tissues.
“Decellularization approaches have been demonstrated for human kidney67, murine kidney67,68, murine heart69,70, human and porcine lung71, and porcine testicular72 tissues, with each type posing unique challenges.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [67]
Some organoids have been grown in decellularized ECM from human or animal donors.
“In order to accurately recapitulate the composition, structure, and vascularization of native ECM in organ development, some organoids have been grown in decellularized ECM from human or animal donors.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [67]
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- Authors
- Orlando, G., Booth, C., Wang, Z., Totonelli, G., Ross, C. L., Moran, E., Salvatori, M., Maghsoudlou, P., Turmaine, M., Delario, G., Al-Shraideh, Y., Farooq, U., Farney, A. C., Rogers, J., Iskandar, S. S., Burns, A., Marini, F. C., De Coppi, P., Stratta, R. J., & Soker, S.
- Affiliations
- Atrium Health Wake Forest Baptist (US)
- Wake Forest University Health Sciences (US)
- Great Ormond Street Hospital (GB)
- University College London (GB)
- Corresponding author
- Orlando, Giuseppe
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2013-08
- Volume
- 34
- Issue
- 24
- Pages
- 5915-5925
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2070781752
- Cited by
- 194
- References
- 41
- Citations by year
- 2026: 7 · 2025: 3 · 2024: 5 · 2023: 11 · 2022: 5 · 2021: 21 · 2020: 12 · 2019: 14 · 2018: 21 · 2017: 25 · 2016: 21 · 2015: 29 · 2014: 16 · 2013: 4
- FWCI
- 22.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Explore Bristol Research (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- renal extracellular matrix, decellularization, kidney regeneration, renal tissue engineering, sodium dodecyl sulfate, angiogenesis, vascular system, kidney transplantation, porcine kidney
- MeSH terms
- Animals, Antigens, Chickens, Extracellular Matrix, Humans, Immunohistochemistry, Kidney, Pressure, Regeneration, Biomarkers, Kidney Transplantation, Neovascularization, Physiologic, Regenerative Medicine, Tissue Scaffolds
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