Long-term nitric oxide release and elevated temperature stability with S-nitroso-N-acetylpenicillamine (SNAP)-doped Elast-eon E2As polymer
Open accessAbstract
Nitric oxide (NO) is known to be a potent inhibitor of platelet activation and adhesion. Healthy endothelial cells that line the inner walls of all blood vessels exhibit a NO flux of 0.5-4 × 10(-10) mol cm(-2) min(-1) that helps prevent thrombosis. Materials with a NO flux that is equivalent to this level are expected to exhibit similar anti-thrombotic properties. In this study, five biomedical grade polymers doped with S-nitroso-N-acetylpenicillamine (SNAP) were investigated for their potential to control the release of NO from the SNAP within the polymers, and further control the release of SNAP itself. SNAP in the Elast-eon E2As polymer creates an inexpensive, homogeneous coating that can locally deliver NO (via thermal and photochemical reactions) as well slowly release SNAP. Furthermore, SNAP is surprisingly stable in the E2As polymer, retaining 82% of the initial SNAP after 2 months storage at 37 °C. The E2As polymer containing SNAP was coated on the walls of extracorporeal circulation (ECC) circuits and exposed to 4 h blood flow in a rabbit model of extracorporeal circulation to examine the effects on platelet count, platelet function, clot area, and fibrinogen adsorption. After 4 h, platelet count was preserved at 100 ± 7% of baseline for the SNAP/E2As coated loops, compared to 60 ± 6% for E2As control circuits (n = 4). The SNAP/E2As coating also reduced the thrombus area when compared to the control (2.3 ± 0.6 and 3.4 ± 1.1 pixels/cm(2), respectively). The results suggest that the new SNAP/E2As coating has potential to improve the thromboresistance of intravascular catheters, grafts, and other blood-contacting medical devices, and exhibits excellent storage stability compared to previously reported NO release polymeric materials.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Because of the large blood-contacting surface area in extracorporeal circulation, loss of platelet count and platelet functionality is significant.
“Due to the large surfaces area that is in contact with blood in extracorporeal circulation (ECC), the loss of platelet count and platelet functionality are significant,161 and many researchers have developed NO releasing43,154,155,161,236,238,278,279 or NO generating254 polymeric ECC tubing to prevent platelet activation and consumption during this process (see Table 1).”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [161]
SNAP-doped E2As polymer films exhibit unprecedented shelf-life stability, with 82% of the initial SNAP remaining after 2 months storage at 37 °C.
“Brisbois et al.161 were the first to discover that SNAP-doped E2As polymer films exhibit unprecedented shelf-life stability, with 82 % of the initial SNAP remaining after 2 months storage at 37 °C.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [161]
10 wt% SNAP/E2As films can release NO for up to 20 d at levels above the physiological NO flux range.
“The 10 wt% SNAP/E2As films can release NO for up to 20 d at levels above the physiological NO flux range.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [161]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Estimation of nitric oxide production and reactionrates in tissue by use of a mathematical model
Vaughn, M. W., Kuo, L., & Liao, J. C. (1998) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1998.274.6.h2163 · Cited by 338 · see citing works
Rassaf, T., Kleinbongard, P., Preik, M., Dejam, A., Gharini, P., Lauer, T., Erckenbrecht, J., Duschin, A., Schulz, R., Heusch, G., et al. (2002) · Circulation Research · doi:10.1161/01.res.0000035038.41739.cb · Cited by 178 · see citing works
Frost, M. C., & Meyerhoff, M. E. (2004) · Journal of the American Chemical Society · doi:10.1021/ja039466i · Cited by 125 · see citing works
Self-citationMajor, T. C., Brant, D. O., Burney, C. P., Amoako, K. A., Annich, G. M., Meyerhoff, M. E., Handa, H., & Bartlett, R. H. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.03.036 · Cited by 118 · see citing works
Self-citationMediation of <i>in vivo</i> glucose sensor inflammatory response via nitric oxide release
Gifford, R., Batchelor, M. M., Lee, Y., Gokulrangan, G., Meyerhoff, M. E., & Wilson, G. S. (2005) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30359 · Cited by 96 · see citing works
Self-citationYang, J., Welby, J. L., & Meyerhoff, M. E. (2008) · Langmuir · doi:10.1021/la801466e · Cited by 55 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Wo, Y., Brisbois, E. J., Bartlett, R. H., & Meyerhoff, M. E. (2016) · Biomaterials Science · doi:10.1039/c6bm00271d · Cited by 251 · see citing works
Self-citationWo, Y., Li, Z., Brisbois, E. J., Colletta, A., Wu, J., Major, T. C., Xi, C., Bartlett, R. H., Matzger, A. J., & Meyerhoff, M. E. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b07501 · Cited by 128 · see citing works
Self-citationBrisbois, E. J., Davis, R. P., Jones, A. M., Major, T. C., Bartlett, R. H., Meyerhoff, M. E., & Handa, H. (2015) · Journal of Materials Chemistry B · doi:10.1039/c4tb01839g · Cited by 101 · see citing works
Self-citationColletta, A., Wu, J., Wo, Y., Kappler, M., Chen, H., Xi, C., & Meyerhoff, M. E. (2015) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.5b00032 · Cited by 89 · see citing works
Self-citationHanda, H., Major, T. C., Brisbois, E. J., Amoako, K. A., Meyerhoff, M. E., & Bartlett, R. H. (2014) · J. Mater. Chem. B · doi:10.1039/c3tb21771j · Cited by 64 · see citing works
Self-citationMajor, T. C., Handa, H., Annich, G. M., & Bartlett, R. H. (2014) · Journal of Biomaterials Applications · doi:10.1177/0885328214538866 · Cited by 43 · see citing works
Self-citationWolf, A. K., Qin, Y., Major, T. C., & Meyerhoff, M. E. (2015) · Chinese Chemical Letters · doi:10.1016/j.cclet.2015.03.002 · Cited by 21 · see citing works
Self-citation
- Authors
- Brisbois, E. J., Handa, H., Major, T. C., Bartlett, R. H., & Meyerhoff, M. E.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Meyerhoff, Mark E.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 34
- Issue
- 28
- Pages
- 6957-6966
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3729938
- OpenAlex
- W2075453630
- Cited by
- 165
- References
- 6
- Citations by year
- 2026: 8 · 2025: 12 · 2024: 11 · 2023: 18 · 2022: 13 · 2021: 16 · 2020: 11 · 2019: 20 · 2018: 12 · 2017: 13 · 2016: 11 · 2015: 13 · 2014: 5 · 2013: 1
- FWCI
- 3.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (EB-004527, K25HL111213)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- nitric oxide release, S-nitroso-N-acetylpenicillamine, SNAP, extracorporeal circuit, thromboresistance, platelet activation, platelet count, thrombus formation, fibrinogen adsorption, blood-contacting devices, storage stability
- MeSH terms
- Animals, Nitric Oxide, Platelet Aggregation, Polymers, Rabbits, Temperature, S-Nitroso-N-Acetylpenicillamine
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
