Mediation of in vivo glucose sensor inflammatory response via nitric oxide release
Abstract
In vivo glucose sensor nitric oxide (NO) release is a means of mediating the inflammatory response that may cause sensor/tissue interactions and degraded sensor performance. The NO release (NOr) sensors were prepared by doping the outer polymeric membrane coating of previously reported needle‐type electrochemical sensors with suitable lipophilic diazeniumdiolate species. The Clarke error grid correlation of sensor glycemia estimates versus blood glucose measured in Sprague‐Dawley rats yielded 99.7% of the points for NOr sensors and 96.3% of points for the control within zones A and B (clinically acceptable) on Day 1, with a similar correlation for Day 3. Histological examination of the implant site demonstrated that the inflammatory response was significantly decreased for 100% of the NOr sensors at 24 h. The NOr sensors also showed a reduced run‐in time of minutes versus hours for control sensors. NO evolution does increase protein nitration in tissue surrounding the sensor, which may be linked to the suppression of inflammation. This study further emphasizes the importance of NO as an electroactive species that can potentially interfere with glucose (peroxide) detection. The NOr sensor offers a viable option for in vivo glucose sensor development. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
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Most biofouling proteins on the sensor membrane were fragments smaller than 15 kDa.
“the majority of the biofouling proteins on the sensor membrane were fragments <15 kDa.102–103”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [103]
The anti-inflammatory effect correlated with nitric oxide release duration, indicating that benefits may only persist while the interface is releasing nitric oxide, in contrast to Hetrick et al. where long-term reduction in inflammation was observed after cessation of release.
“The anti-inflammation correlated with the NO-release duration indicating that the observed benefits may only persist as long as the interface is releasing NO, in contrast to observations by Hetrick et al. where long-term reduction in inflammation were observed after cessation of NO release.103,238”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [103]
The short-term benefits reported by Gifford et al. might be further improved by a larger and more sustained nitric oxide release profile.
“These results indicate that the short-term benefits reported by Gifford et al. may be further enhanced with a greater and more sustained NO release profile.”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [103]
Gifford et al. found that the analytical performance of percutaneous nitric oxide-releasing sensors in rats improved by 2.4% and 2.1% (Clarke Error Grid Analysis) relative to controls on days 1 and 3, respectively.
“the analytical performance of percutaneous NO-releasing sensors in rats was improved by 2.4 and 2.1% (Clarke Error Grid Analysis) for the NO-releasing sensors relative to controls on days 1 and 3, respectively.”
From Biocompatible Materials for Continuous Glucose Monitoring Devices (2013) · cites this paper as [103]
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- Authors
- Gifford, R., Batchelor, M. M., Lee, Y., Gokulrangan, G., Meyerhoff, M. E., & Wilson, G. S.
- Affiliations
- University of Kansas (US)
- University of Michigan (US)
- Corresponding author
- Wilson, George S.
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2005-09
- Volume
- 75A
- Issue
- 4
- Pages
- 755-766
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- OpenAlex
- W2002266135
- Cited by
- 96
- References
- 46
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 6 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 5 · 2016: 6 · 2015: 5 · 2014: 4 · 2013: 11 · 2012: 6
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01dk055297-03)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK55297)
- National Institute of Biomedical Imaging and Bioengineering (EB 00783, R01EB000783)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Keywords
- nitric oxide release, inflammation, diazeniumdiolates, electrochemical glucose sensor, sensor degradation, protein nitration, Clarke error grid, glycemia
- MeSH terms
- Animals, Blood Glucose, Calibration, Electrochemistry, Inflammation, Nitric Oxide, Tyrosine, Biosensing Techniques, Rats, Sprague-Dawley, Rats
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